A comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traits
Abstract Background Head and neck squamous cancer (HNSCC) presents variable phenotype and progression features. Clinically applicable, high-accuracy multifactorial prognostic models for HNSCC survival outcomes are warranted and an active area of research. This study aimed to construct a comprehensiv...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2023-10-01
|
| Series: | Discover Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s12672-023-00796-y |
| _version_ | 1797647295649415168 |
|---|---|
| author | Wen Zeng Fangfang Xie Yiyun Pan Zhengcong Chen Hailong Chen Xiaomei Liu Keqiang Tian Dechang Xu |
| author_facet | Wen Zeng Fangfang Xie Yiyun Pan Zhengcong Chen Hailong Chen Xiaomei Liu Keqiang Tian Dechang Xu |
| author_sort | Wen Zeng |
| collection | DOAJ |
| description | Abstract Background Head and neck squamous cancer (HNSCC) presents variable phenotype and progression features. Clinically applicable, high-accuracy multifactorial prognostic models for HNSCC survival outcomes are warranted and an active area of research. This study aimed to construct a comprehensive prognostic tool for HNSCC overall survival by integrating cancer driver genes with tumor clinical and phenotype information. Methods Key overall survival-related cancer driver genes were screened from among main effector and reciprocal gene pairs using TCGA data using univariate Cox proportional hazard regression analysis. Independent validation was performed using the GSE41613 dataset. The main effector genes among these were selected using LASSO regression and transcriptome score modeling was performed using multivariate Cox regression followed by validation analysis of the prognostic score. Next, multivariate Cox regression analysis was performed using the transcriptome score combined with age, grade, gender, and stage. An ‘Accurate Prediction Model of HNSCC Overall Survival Score’ (APMHO) was computed and validated. Enriched functional pathways, gene mutational landscape, immune cell infiltration, and immunotherapy sensitivity markers associated with high and low APMHO scores were analyzed. Results Screening 107 overall survival-related cancer genes and 402 interacting gene pairs, 6 genes: CRLF2, HSP90AA1, MAP2K1, PAFAH1B2, MYCL and SET genes, were identified and a transcriptional score was obtained. Age, stage and transcriptional score were found to be significant predictors in Cox regression analysis and used to construct a final APMHO model showing an AUC > 0.65 and validated. Transcriptional score, age, pathologic_N, pathologic_T, stage, and TCGA_subtype were significantly different in distribution between high and low APMHO groups. High APMHO samples showed significantly higher mutation rate, enriched tumor-related pathways including Hypoxia, unfold_protein_response, Glycolysis, and mTORC1 signaling, along with differences in immune cell infiltration and immune checkpoint, interferon-γ pathway and m6A regulator expression patterns. Conclusion The APMHO score combining transcriptional and clinical variables showed good prognostic ability for HNSCC overall survival outcomes and was associated with different patterns of phenotypical features, immune and mutational landscape, and immunotherapy sensitivity marker expression. Future studies should validate this score in independent clinical cohorts. |
| first_indexed | 2024-03-11T15:15:13Z |
| format | Article |
| id | doaj.art-df853eb951894195a1377d784208f06e |
| institution | Directory Open Access Journal |
| issn | 2730-6011 |
| language | English |
| last_indexed | 2024-03-11T15:15:13Z |
| publishDate | 2023-10-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj.art-df853eb951894195a1377d784208f06e2023-10-29T12:27:57ZengSpringerDiscover Oncology2730-60112023-10-0114112310.1007/s12672-023-00796-yA comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traitsWen Zeng0Fangfang Xie1Yiyun Pan2Zhengcong Chen3Hailong Chen4Xiaomei Liu5Keqiang Tian6Dechang Xu7Ganzhou Cancer Hospital, Gannan Medical College Affiliated Cancer HospitalGanzhou People’s HospitalGanzhou Cancer Hospital, Gannan Medical College Affiliated Cancer HospitalGanzhou Cancer Hospital, Gannan Medical College Affiliated Cancer HospitalGanzhou Cancer Hospital, Gannan Medical College Affiliated Cancer HospitalGanzhou Cancer Hospital, Gannan Medical College Affiliated Cancer HospitalGanzhou Cancer Hospital, Gannan Medical College Affiliated Cancer HospitalGanzhou Cancer Hospital, Gannan Medical College Affiliated Cancer HospitalAbstract Background Head and neck squamous cancer (HNSCC) presents variable phenotype and progression features. Clinically applicable, high-accuracy multifactorial prognostic models for HNSCC survival outcomes are warranted and an active area of research. This study aimed to construct a comprehensive prognostic tool for HNSCC overall survival by integrating cancer driver genes with tumor clinical and phenotype information. Methods Key overall survival-related cancer driver genes were screened from among main effector and reciprocal gene pairs using TCGA data using univariate Cox proportional hazard regression analysis. Independent validation was performed using the GSE41613 dataset. The main effector genes among these were selected using LASSO regression and transcriptome score modeling was performed using multivariate Cox regression followed by validation analysis of the prognostic score. Next, multivariate Cox regression analysis was performed using the transcriptome score combined with age, grade, gender, and stage. An ‘Accurate Prediction Model of HNSCC Overall Survival Score’ (APMHO) was computed and validated. Enriched functional pathways, gene mutational landscape, immune cell infiltration, and immunotherapy sensitivity markers associated with high and low APMHO scores were analyzed. Results Screening 107 overall survival-related cancer genes and 402 interacting gene pairs, 6 genes: CRLF2, HSP90AA1, MAP2K1, PAFAH1B2, MYCL and SET genes, were identified and a transcriptional score was obtained. Age, stage and transcriptional score were found to be significant predictors in Cox regression analysis and used to construct a final APMHO model showing an AUC > 0.65 and validated. Transcriptional score, age, pathologic_N, pathologic_T, stage, and TCGA_subtype were significantly different in distribution between high and low APMHO groups. High APMHO samples showed significantly higher mutation rate, enriched tumor-related pathways including Hypoxia, unfold_protein_response, Glycolysis, and mTORC1 signaling, along with differences in immune cell infiltration and immune checkpoint, interferon-γ pathway and m6A regulator expression patterns. Conclusion The APMHO score combining transcriptional and clinical variables showed good prognostic ability for HNSCC overall survival outcomes and was associated with different patterns of phenotypical features, immune and mutational landscape, and immunotherapy sensitivity marker expression. Future studies should validate this score in independent clinical cohorts.https://doi.org/10.1007/s12672-023-00796-yHead and neck cancerPrognosisTranscriptomicsCancer genes |
| spellingShingle | Wen Zeng Fangfang Xie Yiyun Pan Zhengcong Chen Hailong Chen Xiaomei Liu Keqiang Tian Dechang Xu A comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traits Discover Oncology Head and neck cancer Prognosis Transcriptomics Cancer genes |
| title | A comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traits |
| title_full | A comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traits |
| title_fullStr | A comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traits |
| title_full_unstemmed | A comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traits |
| title_short | A comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traits |
| title_sort | comprehensive prognostic score for head and neck squamous cancer driver genes and phenotype traits |
| topic | Head and neck cancer Prognosis Transcriptomics Cancer genes |
| url | https://doi.org/10.1007/s12672-023-00796-y |
| work_keys_str_mv | AT wenzeng acomprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT fangfangxie acomprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT yiyunpan acomprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT zhengcongchen acomprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT hailongchen acomprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT xiaomeiliu acomprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT keqiangtian acomprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT dechangxu acomprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT wenzeng comprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT fangfangxie comprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT yiyunpan comprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT zhengcongchen comprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT hailongchen comprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT xiaomeiliu comprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT keqiangtian comprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits AT dechangxu comprehensiveprognosticscoreforheadandnecksquamouscancerdrivergenesandphenotypetraits |