MicroRNAs of the miR-17~92 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression
Macrophages are critically involved in not only immune and inflammatory responses but also maintenance of metabolic fitness of organisms. Combined genetic deficiency of three clusters in the miR-17~92 family drastically shifted macrophage phenotypes toward the inflammatory spectrum characterized by...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-11-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/55676 |
| _version_ | 1811236155479293952 |
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| author | Xiang Zhang Jianguo Liu Li Wu Xiaoyu Hu |
| author_facet | Xiang Zhang Jianguo Liu Li Wu Xiaoyu Hu |
| author_sort | Xiang Zhang |
| collection | DOAJ |
| description | Macrophages are critically involved in not only immune and inflammatory responses but also maintenance of metabolic fitness of organisms. Combined genetic deficiency of three clusters in the miR-17~92 family drastically shifted macrophage phenotypes toward the inflammatory spectrum characterized by heightened production of pro-inflammatory mediator TNF and diminished expression of anti-inflammatory cytokine IL-10. Consequently, macrophages residing in the adipose tissues from myeloid-specific miRNA triple knockout mice spontaneously developed inflammatory phenotypes and displayed alterations of overall physiological conditions as evidenced by obesity and compromised glucose tolerance. Mechanistically, miR-17~92 family miRNAs sustained IL-10 production by promoting transcription of the Fos gene, which is secondary to downregulation of Fos by transcription factor YY1, a direct target of miR-17~92 family miRNAs. Together, these results identified miR-17~92 family miRNAs as crucial regulators of the balance between pro- and anti-inflammatory cytokines and exemplified how macrophage-intrinsic regulatory circuit exerted impactful influence on general physiology. |
| first_indexed | 2024-04-12T12:04:01Z |
| format | Article |
| id | doaj.art-df85e221907249b8bfd1a8a48407d34a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:04:01Z |
| publishDate | 2020-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-df85e221907249b8bfd1a8a48407d34a2022-12-22T03:33:46ZengeLife Sciences Publications LtdeLife2050-084X2020-11-01910.7554/eLife.55676MicroRNAs of the miR-17~92 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expressionXiang Zhang0https://orcid.org/0000-0002-4729-0581Jianguo Liu1Li Wu2Xiaoyu Hu3https://orcid.org/0000-0002-4289-6998Institute for Immunology and School of Medicine, Tsinghua University, Beijing, China; Tsinghua-Peking Centre for Life Sciences, Beijing, China; Beijing Key Laboratory for Immunological Research on Chronic Diseases, Beijing, ChinaDivision of Infectious Diseases, Allergy and Immunology, Department of Internal Medicine, Saint Louis University School of Medicine, Saint Louis University, St. Louis, United StatesInstitute for Immunology and School of Medicine, Tsinghua University, Beijing, China; Tsinghua-Peking Centre for Life Sciences, Beijing, China; Beijing Key Laboratory for Immunological Research on Chronic Diseases, Beijing, ChinaInstitute for Immunology and School of Medicine, Tsinghua University, Beijing, China; Tsinghua-Peking Centre for Life Sciences, Beijing, China; Beijing Key Laboratory for Immunological Research on Chronic Diseases, Beijing, ChinaMacrophages are critically involved in not only immune and inflammatory responses but also maintenance of metabolic fitness of organisms. Combined genetic deficiency of three clusters in the miR-17~92 family drastically shifted macrophage phenotypes toward the inflammatory spectrum characterized by heightened production of pro-inflammatory mediator TNF and diminished expression of anti-inflammatory cytokine IL-10. Consequently, macrophages residing in the adipose tissues from myeloid-specific miRNA triple knockout mice spontaneously developed inflammatory phenotypes and displayed alterations of overall physiological conditions as evidenced by obesity and compromised glucose tolerance. Mechanistically, miR-17~92 family miRNAs sustained IL-10 production by promoting transcription of the Fos gene, which is secondary to downregulation of Fos by transcription factor YY1, a direct target of miR-17~92 family miRNAs. Together, these results identified miR-17~92 family miRNAs as crucial regulators of the balance between pro- and anti-inflammatory cytokines and exemplified how macrophage-intrinsic regulatory circuit exerted impactful influence on general physiology.https://elifesciences.org/articles/55676macrophagesadipose tissueinterleukin-10inflammation |
| spellingShingle | Xiang Zhang Jianguo Liu Li Wu Xiaoyu Hu MicroRNAs of the miR-17~92 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression eLife macrophages adipose tissue interleukin-10 inflammation |
| title | MicroRNAs of the miR-17~92 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression |
| title_full | MicroRNAs of the miR-17~92 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression |
| title_fullStr | MicroRNAs of the miR-17~92 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression |
| title_full_unstemmed | MicroRNAs of the miR-17~92 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression |
| title_short | MicroRNAs of the miR-17~92 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression |
| title_sort | micrornas of the mir 17 92 family maintain adipose tissue macrophage homeostasis by sustaining il 10 expression |
| topic | macrophages adipose tissue interleukin-10 inflammation |
| url | https://elifesciences.org/articles/55676 |
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