Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep Milk
Milk is a typical and satisfactory medium for the growth of lactic acid bacteria (LAB). These microorganisms are of vital importance in the quality of the milk since they contribute to its preservation and give differential organoleptic properties to the final product. Furthermore, LABs can act as b...
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MDPI AG
2020-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/11/2180 |
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| author | Álvaro Rafael Quintana José Manuel Perea María Llanos Palop Ana Garzón Ramón Arias |
| author_facet | Álvaro Rafael Quintana José Manuel Perea María Llanos Palop Ana Garzón Ramón Arias |
| author_sort | Álvaro Rafael Quintana |
| collection | DOAJ |
| description | Milk is a typical and satisfactory medium for the growth of lactic acid bacteria (LAB). These microorganisms are of vital importance in the quality of the milk since they contribute to its preservation and give differential organoleptic properties to the final product. Furthermore, LABs can act as biocontrol agents in the dairy industry by inhibiting the growth of undesirable bacteria present in milk and by improving the quality of dairy products such as cheese. In this context, knowing the transfer routes used by LABs from the livestock environment to the milk is of great importance within the dairy industry. Therefore, the objectives of the present study were to expand the knowledge of the LAB population present in the milk of Manchego ewe by means of DNA sequencing techniques and to evaluate the possible transfers of LAB species based on the management of each dairy farm. Samples of bulk tank milk, air (from the milking parlour and from the livestock housing), animal feed and teat surface (taken from 10 sheep per farm) were collected in 12 traditional livestock farms in Castilla-La Mancha (Spain), where each farm presented differences regarding their farming practices. A mixed-effects model was used to evaluate the effects of livestock practices on the distribution of LAB species. Results showed that the vast majority of species identified in the milk had an isolate that was also found in other matrices, which could indicate a microbial transference via the livestock environment to the milk. In addition, the mixed model showed that the factors that positively influence the LAB count were the low-line milking system and the daily use of acid detergent in cleaning the milking machine. |
| first_indexed | 2024-03-10T14:40:23Z |
| format | Article |
| id | doaj.art-df89e98628bb42f9ad6de38e60dd3306 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T14:40:23Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-df89e98628bb42f9ad6de38e60dd33062023-11-20T21:52:30ZengMDPI AGAnimals2076-26152020-11-011011218010.3390/ani10112180Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep MilkÁlvaro Rafael Quintana0José Manuel Perea1María Llanos Palop2Ana Garzón3Ramón Arias4Regional Institute of Agrifood and Forestry Research and Development of Castilla-La Mancha (IRIAF), CERSYRA Valdepeñas, 13300 Ciudad Real, SpainDepartment of Animal Production, University of Córdoba, 14071 Córdoba, SpainDepartment of Analytical Chemistry and Food Technology, Faculty of Environmental Sciences and Biochemistry, University of Castilla-La Mancha, 45071 Toledo, SpainDepartment of Animal Production, University of Córdoba, 14071 Córdoba, SpainRegional Institute of Agrifood and Forestry Research and Development of Castilla-La Mancha (IRIAF), CERSYRA Valdepeñas, 13300 Ciudad Real, SpainMilk is a typical and satisfactory medium for the growth of lactic acid bacteria (LAB). These microorganisms are of vital importance in the quality of the milk since they contribute to its preservation and give differential organoleptic properties to the final product. Furthermore, LABs can act as biocontrol agents in the dairy industry by inhibiting the growth of undesirable bacteria present in milk and by improving the quality of dairy products such as cheese. In this context, knowing the transfer routes used by LABs from the livestock environment to the milk is of great importance within the dairy industry. Therefore, the objectives of the present study were to expand the knowledge of the LAB population present in the milk of Manchego ewe by means of DNA sequencing techniques and to evaluate the possible transfers of LAB species based on the management of each dairy farm. Samples of bulk tank milk, air (from the milking parlour and from the livestock housing), animal feed and teat surface (taken from 10 sheep per farm) were collected in 12 traditional livestock farms in Castilla-La Mancha (Spain), where each farm presented differences regarding their farming practices. A mixed-effects model was used to evaluate the effects of livestock practices on the distribution of LAB species. Results showed that the vast majority of species identified in the milk had an isolate that was also found in other matrices, which could indicate a microbial transference via the livestock environment to the milk. In addition, the mixed model showed that the factors that positively influence the LAB count were the low-line milking system and the daily use of acid detergent in cleaning the milking machine.https://www.mdpi.com/2076-2615/10/11/2180lactic acid bacteriaLABdairy farm environmentewe’s milkfarming practices |
| spellingShingle | Álvaro Rafael Quintana José Manuel Perea María Llanos Palop Ana Garzón Ramón Arias Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep Milk Animals lactic acid bacteria LAB dairy farm environment ewe’s milk farming practices |
| title | Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep Milk |
| title_full | Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep Milk |
| title_fullStr | Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep Milk |
| title_full_unstemmed | Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep Milk |
| title_short | Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep Milk |
| title_sort | influence of environmental and productive factors on the biodiversity of lactic acid bacteria population from sheep milk |
| topic | lactic acid bacteria LAB dairy farm environment ewe’s milk farming practices |
| url | https://www.mdpi.com/2076-2615/10/11/2180 |
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