Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virus
The infectious full-length cDNA clone of zucchini yellow mosaic virus (ZYMV) isolate PA (pZYMV-PA), which was isolated from pumpkin, was constructed by utilizing viral transcription and processing signals to produce infectious in vivo transcripts. Simple rub-inoculation of plasmid DNAs of pZYMV-PA w...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2016-02-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4755678 |
| _version_ | 1811231830058205184 |
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| author | Minji Kang Jang-Kyun Seo Hoseong Choi Hong-Soo Choi Kook-Hyung Kim |
| author_facet | Minji Kang Jang-Kyun Seo Hoseong Choi Hong-Soo Choi Kook-Hyung Kim |
| author_sort | Minji Kang |
| collection | DOAJ |
| description | The infectious full-length cDNA clone of zucchini yellow mosaic virus (ZYMV) isolate PA (pZYMV-PA), which was isolated from pumpkin, was constructed by utilizing viral transcription and processing signals to produce infectious in vivo transcripts. Simple rub-inoculation of plasmid DNAs of pZYMV-PA was successful to cause infection of zucchini plants (Cucurbita pepo L.). We further engineered this infectious cDNA clone of ZYMV as a viral vector for systemic expression of heterologous proteins in cucurbits. We successfully expressed two reporter genes including gfp and bar in zucchini plants by simple rub-inoculation of plasmid DNAs of the ZYMV-based expression constructs. Our method of the ZYMV-based viral vector in association with the simple rub-inoculation provides an easy and rapid approach for introduction and evaluation of heterologous genes in cucurbits. |
| first_indexed | 2024-04-12T10:51:40Z |
| format | Article |
| id | doaj.art-df8a6478ece842ee98e457a39d66364b |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-04-12T10:51:40Z |
| publishDate | 2016-02-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-df8a6478ece842ee98e457a39d66364b2022-12-22T03:36:13ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542016-02-01321707610.5423/PPJ.NT.08.2015.0173PPJ.NT.08.2015.0173Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virusMinji Kang0Jang-Kyun Seo1Hoseong Choi2Hong-Soo Choi3Kook-Hyung Kim4Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaCrop Protection Division, National Academy of Agricultural Science, Wanju 565-852, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaCrop Protection Division, National Academy of Agricultural Science, Wanju 565-852, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaThe infectious full-length cDNA clone of zucchini yellow mosaic virus (ZYMV) isolate PA (pZYMV-PA), which was isolated from pumpkin, was constructed by utilizing viral transcription and processing signals to produce infectious in vivo transcripts. Simple rub-inoculation of plasmid DNAs of pZYMV-PA was successful to cause infection of zucchini plants (Cucurbita pepo L.). We further engineered this infectious cDNA clone of ZYMV as a viral vector for systemic expression of heterologous proteins in cucurbits. We successfully expressed two reporter genes including gfp and bar in zucchini plants by simple rub-inoculation of plasmid DNAs of the ZYMV-based expression constructs. Our method of the ZYMV-based viral vector in association with the simple rub-inoculation provides an easy and rapid approach for introduction and evaluation of heterologous genes in cucurbits.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4755678gene deliveryinfectious cloneZYMV |
| spellingShingle | Minji Kang Jang-Kyun Seo Hoseong Choi Hong-Soo Choi Kook-Hyung Kim Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virus The Plant Pathology Journal gene delivery infectious clone ZYMV |
| title | Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virus |
| title_full | Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virus |
| title_fullStr | Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virus |
| title_full_unstemmed | Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virus |
| title_short | Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virus |
| title_sort | establishment of a simple and rapid gene delivery system for cucurbits by using engineered of zucchini yellow mosaic virus |
| topic | gene delivery infectious clone ZYMV |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4755678 |
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