Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey
Background/Purpose: The aim of the present study was to estimate the prevalence of Fasciola hepatica infection in sheep in the Black Sea region of Turkey. Methods: Samples from 213 sheep were collected randomly in Samsun, Tokat, and Sinop from September 2005 to January 2007 and tested by indirect en...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-06-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118215008038 |
| _version_ | 1811217604121985024 |
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| author | Mustafa Acici Ozlem Buyuktanir Cenk Soner Bolukbas Gokmen Zafer Pekmezci Ali Tumay Gurler Sinasi Umur |
| author_facet | Mustafa Acici Ozlem Buyuktanir Cenk Soner Bolukbas Gokmen Zafer Pekmezci Ali Tumay Gurler Sinasi Umur |
| author_sort | Mustafa Acici |
| collection | DOAJ |
| description | Background/Purpose: The aim of the present study was to estimate the prevalence of Fasciola hepatica infection in sheep in the Black Sea region of Turkey.
Methods: Samples from 213 sheep were collected randomly in Samsun, Tokat, and Sinop from September 2005 to January 2007 and tested by indirect enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using F. hepatica excretory-secretory (E/S) antigens.
Results: The distribution of ELISA-positive samples for F. hepatica infections out of a total of 213 sheep serum samples was 23/71 (32.4%), 15/59 (25.4%), and 29/83 (34.9%) in Samsun, Sinop, and Tokat, respectively. The immunodominant proteins were determined by Western blot analysis using molecular weight markers of 14 kDa, 20 kDa, 24 kDa, 27 kDa, 33 kDa, 45 kDa, and 66 kDa and extracted from sera of sheep that were positive for Fasciola spp. eggs and also hyperimmune sera from rabbits immunized with E/S antigens.
Conclusion: The ELISA-positive results were confirmed by Western blot analysis. As a result, seroprevalence of F. hepatica infection was found in 31.4% of sheep from the Karayaka breed in the Middle Black sea region of Turkey. |
| first_indexed | 2024-04-12T06:57:36Z |
| format | Article |
| id | doaj.art-df8c38ccd4404eb3a89bc99436a124e7 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-04-12T06:57:36Z |
| publishDate | 2017-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-df8c38ccd4404eb3a89bc99436a124e72022-12-22T03:43:06ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822017-06-0150337738110.1016/j.jmii.2015.06.005Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of TurkeyMustafa Acici0Ozlem Buyuktanir1Cenk Soner Bolukbas2Gokmen Zafer Pekmezci3Ali Tumay Gurler4Sinasi Umur5Department of Parasitology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, TurkeyDepartment of Microbiology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, TurkeyDepartment of Parasitology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, TurkeyDepartment of Preclinical Sciences, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, TurkeyDepartment of Parasitology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, TurkeyDepartment of Parasitology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, TurkeyBackground/Purpose: The aim of the present study was to estimate the prevalence of Fasciola hepatica infection in sheep in the Black Sea region of Turkey. Methods: Samples from 213 sheep were collected randomly in Samsun, Tokat, and Sinop from September 2005 to January 2007 and tested by indirect enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using F. hepatica excretory-secretory (E/S) antigens. Results: The distribution of ELISA-positive samples for F. hepatica infections out of a total of 213 sheep serum samples was 23/71 (32.4%), 15/59 (25.4%), and 29/83 (34.9%) in Samsun, Sinop, and Tokat, respectively. The immunodominant proteins were determined by Western blot analysis using molecular weight markers of 14 kDa, 20 kDa, 24 kDa, 27 kDa, 33 kDa, 45 kDa, and 66 kDa and extracted from sera of sheep that were positive for Fasciola spp. eggs and also hyperimmune sera from rabbits immunized with E/S antigens. Conclusion: The ELISA-positive results were confirmed by Western blot analysis. As a result, seroprevalence of F. hepatica infection was found in 31.4% of sheep from the Karayaka breed in the Middle Black sea region of Turkey.http://www.sciencedirect.com/science/article/pii/S1684118215008038ELISAfasciolosissheepTurkey |
| spellingShingle | Mustafa Acici Ozlem Buyuktanir Cenk Soner Bolukbas Gokmen Zafer Pekmezci Ali Tumay Gurler Sinasi Umur Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey Journal of Microbiology, Immunology and Infection ELISA fasciolosis sheep Turkey |
| title | Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey |
| title_full | Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey |
| title_fullStr | Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey |
| title_full_unstemmed | Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey |
| title_short | Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey |
| title_sort | serologic detection of antibodies against fasciola hepatica in sheep in the middle black sea region of turkey |
| topic | ELISA fasciolosis sheep Turkey |
| url | http://www.sciencedirect.com/science/article/pii/S1684118215008038 |
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