Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells
Bone marrow chimaeric mice are a valuable tool in research, but require myeloablative conditioning. Here the authors demonstrate efficient FACS-free enrichment of haematopoietic stem and progenitor cells for transplantation into unconditioned recipient mice, as well as for genetic engineering using...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-23763-z |
| _version_ | 1818858435012198400 |
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| author | Kiyosumi Ochi Maiko Morita Adam C. Wilkinson Atsushi Iwama Satoshi Yamazaki |
| author_facet | Kiyosumi Ochi Maiko Morita Adam C. Wilkinson Atsushi Iwama Satoshi Yamazaki |
| author_sort | Kiyosumi Ochi |
| collection | DOAJ |
| description | Bone marrow chimaeric mice are a valuable tool in research, but require myeloablative conditioning. Here the authors demonstrate efficient FACS-free enrichment of haematopoietic stem and progenitor cells for transplantation into unconditioned recipient mice, as well as for genetic engineering using polyvinyl alcohol based media. |
| first_indexed | 2024-12-19T08:56:14Z |
| format | Article |
| id | doaj.art-df8da65f907e470fb2d843f89f224190 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T08:56:14Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-df8da65f907e470fb2d843f89f2241902022-12-21T20:28:37ZengNature PortfolioNature Communications2041-17232021-06-0112111010.1038/s41467-021-23763-zNon-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cellsKiyosumi Ochi0Maiko Morita1Adam C. Wilkinson2Atsushi Iwama3Satoshi Yamazaki4Division of Stem Cell Biology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of TokyoDivision of Stem Cell Biology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of TokyoMRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Radcliffe Department of Medicine, University of OxfordDivision of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of TokyoDivision of Stem Cell Biology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of TokyoBone marrow chimaeric mice are a valuable tool in research, but require myeloablative conditioning. Here the authors demonstrate efficient FACS-free enrichment of haematopoietic stem and progenitor cells for transplantation into unconditioned recipient mice, as well as for genetic engineering using polyvinyl alcohol based media.https://doi.org/10.1038/s41467-021-23763-z |
| spellingShingle | Kiyosumi Ochi Maiko Morita Adam C. Wilkinson Atsushi Iwama Satoshi Yamazaki Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells Nature Communications |
| title | Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells |
| title_full | Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells |
| title_fullStr | Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells |
| title_full_unstemmed | Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells |
| title_short | Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells |
| title_sort | non conditioned bone marrow chimeric mouse generation using culture based enrichment of hematopoietic stem and progenitor cells |
| url | https://doi.org/10.1038/s41467-021-23763-z |
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