Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese Women
The metabolic syndrome (MetS) is a cluster of the most dangerous heart attack risk factors: diabetes or raised fasting plasma glucose, abdominal obesity, high cholesterol and high blood pressure. The goal of this study is to compare the state of the main features of obesity-associated white adipose...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/4/2394 |
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| author | Óscar Osorio-Conles Arturo Vega-Beyhart Ainitze Ibarzabal José María Balibrea Josep Vidal Ana de Hollanda |
| author_facet | Óscar Osorio-Conles Arturo Vega-Beyhart Ainitze Ibarzabal José María Balibrea Josep Vidal Ana de Hollanda |
| author_sort | Óscar Osorio-Conles |
| collection | DOAJ |
| description | The metabolic syndrome (MetS) is a cluster of the most dangerous heart attack risk factors: diabetes or raised fasting plasma glucose, abdominal obesity, high cholesterol and high blood pressure. The goal of this study is to compare the state of the main features of obesity-associated white adipose tissue (WAT) dysfunction in 66 women with severe obesity without (MetS−) or with MetS (MetS+). Fat cell area, adipocyte size distribution and histological fibrosis were analysed in visceral (VAT) and abdominal subcutaneous WAT (SAT) in 33 age- and BMI-matched pairs of MetS− and MetS+ subjects. The mRNA expression of 93 genes implicated in obesity-associated WAT dysfunction was analysed by RT-qPCR in both fat depots. MetS+ females showed higher adipocyte hypertrophy in both fat depots and increased fibrosis and expression of macrophage and hypoxia markers in SAT. Transcriptional data suggest increased fatty acid oxidation in SAT and impaired thermogenesis and extracellular matrix remodelling in VAT from MetS+ subjects. A sPLS-DA model, including SAT expression of <i>PPARA</i> and <i>LEPR</i> genes identified MetS with an AUC = 0.87. Despite equal age, BMI and body composition, MetS+ females display morphological and transcriptional differences in both WAT depots, especially in SAT. These factors may contribute to the transition to MetS. |
| first_indexed | 2024-03-09T21:43:33Z |
| format | Article |
| id | doaj.art-df9768a60a224ca0ae0155068aa74724 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:43:33Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-df9768a60a224ca0ae0155068aa747242023-11-23T20:24:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01234239410.3390/ijms23042394Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese WomenÓscar Osorio-Conles0Arturo Vega-Beyhart1Ainitze Ibarzabal2José María Balibrea3Josep Vidal4Ana de Hollanda5Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, SpainInstitut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, SpainGastrointestinal Surgery Department, Hospital Clínic de Barcelona, 08036 Barcelona, SpainGastrointestinal Surgery Department, Hospital Clínic de Barcelona, 08036 Barcelona, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, SpainInstitut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, SpainThe metabolic syndrome (MetS) is a cluster of the most dangerous heart attack risk factors: diabetes or raised fasting plasma glucose, abdominal obesity, high cholesterol and high blood pressure. The goal of this study is to compare the state of the main features of obesity-associated white adipose tissue (WAT) dysfunction in 66 women with severe obesity without (MetS−) or with MetS (MetS+). Fat cell area, adipocyte size distribution and histological fibrosis were analysed in visceral (VAT) and abdominal subcutaneous WAT (SAT) in 33 age- and BMI-matched pairs of MetS− and MetS+ subjects. The mRNA expression of 93 genes implicated in obesity-associated WAT dysfunction was analysed by RT-qPCR in both fat depots. MetS+ females showed higher adipocyte hypertrophy in both fat depots and increased fibrosis and expression of macrophage and hypoxia markers in SAT. Transcriptional data suggest increased fatty acid oxidation in SAT and impaired thermogenesis and extracellular matrix remodelling in VAT from MetS+ subjects. A sPLS-DA model, including SAT expression of <i>PPARA</i> and <i>LEPR</i> genes identified MetS with an AUC = 0.87. Despite equal age, BMI and body composition, MetS+ females display morphological and transcriptional differences in both WAT depots, especially in SAT. These factors may contribute to the transition to MetS.https://www.mdpi.com/1422-0067/23/4/2394metabolic syndromeobesitysubcutaneous adipose tissuevisceral adipose tissue |
| spellingShingle | Óscar Osorio-Conles Arturo Vega-Beyhart Ainitze Ibarzabal José María Balibrea Josep Vidal Ana de Hollanda Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese Women International Journal of Molecular Sciences metabolic syndrome obesity subcutaneous adipose tissue visceral adipose tissue |
| title | Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese Women |
| title_full | Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese Women |
| title_fullStr | Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese Women |
| title_full_unstemmed | Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese Women |
| title_short | Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese Women |
| title_sort | biological determinants of metabolic syndrome in visceral and subcutaneous adipose tissue from severely obese women |
| topic | metabolic syndrome obesity subcutaneous adipose tissue visceral adipose tissue |
| url | https://www.mdpi.com/1422-0067/23/4/2394 |
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