Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteins
Abstract Fluorescence photoswitch is becoming increasingly desirable for many applications, but its controllable fabrication still remains challenging yet. In this paper, a new strategy is proposed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | Aggregate |
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| Online Access: | https://doi.org/10.1002/agt2.245 |
| _version_ | 1797902607394537472 |
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| author | Wencheng Zhong Xiaojian Yan Shaohua Qu Li Shang |
| author_facet | Wencheng Zhong Xiaojian Yan Shaohua Qu Li Shang |
| author_sort | Wencheng Zhong |
| collection | DOAJ |
| description | Abstract Fluorescence photoswitch is becoming increasingly desirable for many applications, but its controllable fabrication still remains challenging yet. In this paper, a new strategy is proposed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome carriers and biotemplates of fluorophore. As an example, we demonstrated the successful fabrication of a fluorescence photoswitch by incorporating both the photochromic diarylethene dye (CMTE) and red‐emitting fluorescent gold nanoclusters (AuNCs) into the specific domains of bovine serum albumin (BSA) in a highly controllable manner. Detailed spectral and photophysical characterisation showed that CMTE well‐retains the photochromic properties within the CMTE–BSA–AuNC construct, although its photoconversion rate is slightly retarded. Different from previously reported photoswitches, the fluorescence of the present system is mainly modulated via the inner filter effect (IFE) mechanism, which exhibits high switching efficiency with an on–off ratio up to 90%, reversible fluorescence response and good antifatigue performance. This work provides a new, generable albumin protein‐assisted strategy of fabricating advanced fluorescence photoswitch, which can find wide applications in biological, optical and information fields. |
| first_indexed | 2024-04-10T09:20:19Z |
| format | Article |
| id | doaj.art-df97cfeff009490198870f0aee6af78f |
| institution | Directory Open Access Journal |
| issn | 2692-4560 |
| language | English |
| last_indexed | 2024-04-10T09:20:19Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aggregate |
| spelling | doaj.art-df97cfeff009490198870f0aee6af78f2023-02-20T15:03:57ZengWileyAggregate2692-45602023-02-0141n/an/a10.1002/agt2.245Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteinsWencheng Zhong0Xiaojian Yan1Shaohua Qu2Li Shang3State Key Laboratory of Solidification Processing, School of Materials Science and Engineering Northwestern Polytechnical University and Shaanxi Joint Laboratory of Graphene (NPU) Xi'an ChinaState Key Laboratory of Solidification Processing, School of Materials Science and Engineering Northwestern Polytechnical University and Shaanxi Joint Laboratory of Graphene (NPU) Xi'an ChinaState Key Laboratory of Solidification Processing, School of Materials Science and Engineering Northwestern Polytechnical University and Shaanxi Joint Laboratory of Graphene (NPU) Xi'an ChinaState Key Laboratory of Solidification Processing, School of Materials Science and Engineering Northwestern Polytechnical University and Shaanxi Joint Laboratory of Graphene (NPU) Xi'an ChinaAbstract Fluorescence photoswitch is becoming increasingly desirable for many applications, but its controllable fabrication still remains challenging yet. In this paper, a new strategy is proposed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome carriers and biotemplates of fluorophore. As an example, we demonstrated the successful fabrication of a fluorescence photoswitch by incorporating both the photochromic diarylethene dye (CMTE) and red‐emitting fluorescent gold nanoclusters (AuNCs) into the specific domains of bovine serum albumin (BSA) in a highly controllable manner. Detailed spectral and photophysical characterisation showed that CMTE well‐retains the photochromic properties within the CMTE–BSA–AuNC construct, although its photoconversion rate is slightly retarded. Different from previously reported photoswitches, the fluorescence of the present system is mainly modulated via the inner filter effect (IFE) mechanism, which exhibits high switching efficiency with an on–off ratio up to 90%, reversible fluorescence response and good antifatigue performance. This work provides a new, generable albumin protein‐assisted strategy of fabricating advanced fluorescence photoswitch, which can find wide applications in biological, optical and information fields.https://doi.org/10.1002/agt2.245albumin proteinfluorescence switchgold nanoclustersinner filter effectphotochrome |
| spellingShingle | Wencheng Zhong Xiaojian Yan Shaohua Qu Li Shang Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteins Aggregate albumin protein fluorescence switch gold nanoclusters inner filter effect photochrome |
| title | Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteins |
| title_full | Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteins |
| title_fullStr | Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteins |
| title_full_unstemmed | Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteins |
| title_short | Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteins |
| title_sort | site specific fabrication of gold nanocluster based fluorescence photoswitch enabled by the dual roles of albumin proteins |
| topic | albumin protein fluorescence switch gold nanoclusters inner filter effect photochrome |
| url | https://doi.org/10.1002/agt2.245 |
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