CRISPR/Cas13a combined with reverse transcription and RPA for NoV GII.4 monitoring in water environments

CRISPR/Cas13a combined with reverse transcription and RPA for NoV GII.4 monitoring in water environments

Water bodies contaminated with the norovirus (NoV) are important vectors for its transmission. Therefore, enhanced monitoring of NoV in aqueous environments plays an active role in preventing diseases. Here, we reverse transcribed viral RNA into cDNA, and then used the constructed RPA-CRISPR/Cas13a-...

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Bibliographic Details
Main Authors: Yiqiang Sun, Weiwei Zhang, Houyun Zhang, Feng Zhao, Laijin Su
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Environment International
Subjects:
Water environments
NoV GII.4
RPA-CRISPR/Cas13a
Quantitative assay
Public health
Online Access:http://www.sciencedirect.com/science/article/pii/S0160412024007815

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http://www.sciencedirect.com/science/article/pii/S0160412024007815

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