Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique
Intestinal injury owing to inflammation, severe trauma, and burn is a leading cause of morbidity and mortality. Currently, animal models employed to study the intestinal response to injury and inflammation depend on outdated methods of analysis. Given that these classic intestinal assays are lethal...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publications
2010-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2010.00001 |
| _version_ | 1797282391354507264 |
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| author | Todd W. Costantini Brian P. Eliceiri Carrie Y. Peterson William H. Loomis James G. Putnam Andrew Baird Paul Wolf Vishal Bansal Raul Coimbra |
| author_facet | Todd W. Costantini Brian P. Eliceiri Carrie Y. Peterson William H. Loomis James G. Putnam Andrew Baird Paul Wolf Vishal Bansal Raul Coimbra |
| author_sort | Todd W. Costantini |
| collection | DOAJ |
| description | Intestinal injury owing to inflammation, severe trauma, and burn is a leading cause of morbidity and mortality. Currently, animal models employed to study the intestinal response to injury and inflammation depend on outdated methods of analysis. Given that these classic intestinal assays are lethal to the experimental animal, there is no ability to study the gut response to injury in the same animal over time. We postulated that by developing an in vivo assay to image intestinal injury using fluorescent dye, it could complement other expensive, time-consuming, and semiquantitative classic means of detecting intestinal injury. We describe a novel in vivo, noninvasive method to image intestinal injury using a charge-coupled device (CCD) camera that allows for serial visual and quantitative analysis of intestinal injury. Our results correlate with traditional, time–consuming, semiquantitative assays of intestinal injury, now allowing the noninvasive, nonlethal assessment of injury over time. |
| first_indexed | 2024-03-07T17:12:16Z |
| format | Article |
| id | doaj.art-df9b93311e6b4e83bca0ca9e78e08e63 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T17:12:16Z |
| publishDate | 2010-01-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-df9b93311e6b4e83bca0ca9e78e08e632024-03-03T00:58:35ZengSAGE PublicationsMolecular Imaging1536-01212010-01-01910.2310/7290.2010.0000110.2310_7290.2010.00001Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging TechniqueTodd W. CostantiniBrian P. EliceiriCarrie Y. PetersonWilliam H. LoomisJames G. PutnamAndrew BairdPaul WolfVishal BansalRaul CoimbraIntestinal injury owing to inflammation, severe trauma, and burn is a leading cause of morbidity and mortality. Currently, animal models employed to study the intestinal response to injury and inflammation depend on outdated methods of analysis. Given that these classic intestinal assays are lethal to the experimental animal, there is no ability to study the gut response to injury in the same animal over time. We postulated that by developing an in vivo assay to image intestinal injury using fluorescent dye, it could complement other expensive, time-consuming, and semiquantitative classic means of detecting intestinal injury. We describe a novel in vivo, noninvasive method to image intestinal injury using a charge-coupled device (CCD) camera that allows for serial visual and quantitative analysis of intestinal injury. Our results correlate with traditional, time–consuming, semiquantitative assays of intestinal injury, now allowing the noninvasive, nonlethal assessment of injury over time.https://doi.org/10.2310/7290.2010.00001 |
| spellingShingle | Todd W. Costantini Brian P. Eliceiri Carrie Y. Peterson William H. Loomis James G. Putnam Andrew Baird Paul Wolf Vishal Bansal Raul Coimbra Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique Molecular Imaging |
| title | Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique |
| title_full | Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique |
| title_fullStr | Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique |
| title_full_unstemmed | Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique |
| title_short | Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique |
| title_sort | quantitative assessment of intestinal injury using a novel in vivo near infrared imaging technique |
| url | https://doi.org/10.2310/7290.2010.00001 |
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