Bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in Punjab, India
The unprecedented application of pesticides in Punjab, India during green revolution has lead to an environmental crisis due to the accumulation of persistent organic and pesticide pollutants in the environment and biota of this region. The present study aimed at estimating the abundance of pesticid...
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Elsevier
2020-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020321174 |
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| author | Siddharth Gautam Naresh Kumar Sood Kuldip Gupta Chitra Joshi Kamalpreet Kaur Gill Rajdeep Kaur Indrasen Chauhan |
| author_facet | Siddharth Gautam Naresh Kumar Sood Kuldip Gupta Chitra Joshi Kamalpreet Kaur Gill Rajdeep Kaur Indrasen Chauhan |
| author_sort | Siddharth Gautam |
| collection | DOAJ |
| description | The unprecedented application of pesticides in Punjab, India during green revolution has lead to an environmental crisis due to the accumulation of persistent organic and pesticide pollutants in the environment and biota of this region. The present study aimed at estimating the abundance of pesticide contaminants in three biological matrices of 36 dogs suffering from malignant canine mammary tumor (mCMT) and 6 tumor free control dogs from Punjab, India. Presence of individual and total pesticides in canine biological samples, age and bodyweight of canine patients was assessed as a potential risk factor for mCMT using logistic regression analysis. Chi-square test was employed to determine tissue-specific accumulations of individual pesticides. Spearman's correlation coefficient was estimated to determine the association between the levels of total pesticides in different tissue matrices and with age and bodyweight of mCMT cases. Gas chromatography-ECD analysis of serum, mammary tissue and adjoining mammary adipose tissue revealed fourteen different pesticides including γ-HCH, α-HCH, dieldrin, aldrin, heptachlor, butachlor, p,p-DDT, o,p-DDT, p,p-DDD, p,p-DDE, L-cyhalothrin, permethrin, fipronil, and fenitrothion. Heptachlor, γ-HCH, aldrin and p,p-DDT were more frequently detected, whereas, p,p-DDE and o,p-DDT were the least common. Differential accumulation of pesticides in tissue matrices, particularly between serum and mammary tissue/adipose tissue was observed. We could not find any association between the total pesticide concentrations among serum, mammary tissue and mammary adipose tissue in mCMT cases. We found that the odds for individual pesticide for serum, mammary tissue and adipose tissue were associated with high uncertainties; however, the total pesticide concentration in mammary tissue was near non-significantly associated with higher risk of mCMT with low uncertainty. Statistically non-significant higher odds of CMT occurrence with increase in age was noticed No association between the concentration of total pesticides in different matrices and age and bodyweight of canine subjects was found. |
| first_indexed | 2024-12-20T05:41:51Z |
| format | Article |
| id | doaj.art-df9d1277b3564f9db027306fb689a089 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-20T05:41:51Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-df9d1277b3564f9db027306fb689a0892022-12-21T19:51:26ZengElsevierHeliyon2405-84402020-10-01610e05274Bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in Punjab, IndiaSiddharth Gautam0Naresh Kumar Sood1Kuldip Gupta2Chitra Joshi3Kamalpreet Kaur Gill4Rajdeep Kaur5Indrasen Chauhan6Division of Temperate Animal Husbandry, Indian Veterinary Research Institute, Mukteshwar 263138, Nainital, Uttarakhand, India; Corresponding author.Department of Teaching Veterinary Clinical Complex, GADVASU 141004, Ludhiana, Punjab, IndiaDepartment of Veterinary Pathology, GADVASU 141004, Ludhiana, Punjab, IndiaDepartment of Animal Husbandry, Almora 263601, Uttarakhand, IndiaDepartment of Veterinary Pharmacology & Toxicology, GADVASU 141004, Ludhiana, Punjab, IndiaDepartment of Veterinary Pharmacology & Toxicology, GADVASU 141004, Ludhiana, Punjab, IndiaDivision of Temperate Animal Husbandry, Indian Veterinary Research Institute, Mukteshwar 263138, Nainital, Uttarakhand, IndiaThe unprecedented application of pesticides in Punjab, India during green revolution has lead to an environmental crisis due to the accumulation of persistent organic and pesticide pollutants in the environment and biota of this region. The present study aimed at estimating the abundance of pesticide contaminants in three biological matrices of 36 dogs suffering from malignant canine mammary tumor (mCMT) and 6 tumor free control dogs from Punjab, India. Presence of individual and total pesticides in canine biological samples, age and bodyweight of canine patients was assessed as a potential risk factor for mCMT using logistic regression analysis. Chi-square test was employed to determine tissue-specific accumulations of individual pesticides. Spearman's correlation coefficient was estimated to determine the association between the levels of total pesticides in different tissue matrices and with age and bodyweight of mCMT cases. Gas chromatography-ECD analysis of serum, mammary tissue and adjoining mammary adipose tissue revealed fourteen different pesticides including γ-HCH, α-HCH, dieldrin, aldrin, heptachlor, butachlor, p,p-DDT, o,p-DDT, p,p-DDD, p,p-DDE, L-cyhalothrin, permethrin, fipronil, and fenitrothion. Heptachlor, γ-HCH, aldrin and p,p-DDT were more frequently detected, whereas, p,p-DDE and o,p-DDT were the least common. Differential accumulation of pesticides in tissue matrices, particularly between serum and mammary tissue/adipose tissue was observed. We could not find any association between the total pesticide concentrations among serum, mammary tissue and mammary adipose tissue in mCMT cases. We found that the odds for individual pesticide for serum, mammary tissue and adipose tissue were associated with high uncertainties; however, the total pesticide concentration in mammary tissue was near non-significantly associated with higher risk of mCMT with low uncertainty. Statistically non-significant higher odds of CMT occurrence with increase in age was noticed No association between the concentration of total pesticides in different matrices and age and bodyweight of canine subjects was found.http://www.sciencedirect.com/science/article/pii/S2405844020321174Cancer researchEnvironmental scienceToxicologyVeterinary medicineClinical researchClinical toxicology |
| spellingShingle | Siddharth Gautam Naresh Kumar Sood Kuldip Gupta Chitra Joshi Kamalpreet Kaur Gill Rajdeep Kaur Indrasen Chauhan Bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in Punjab, India Heliyon Cancer research Environmental science Toxicology Veterinary medicine Clinical research Clinical toxicology |
| title | Bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in Punjab, India |
| title_full | Bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in Punjab, India |
| title_fullStr | Bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in Punjab, India |
| title_full_unstemmed | Bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in Punjab, India |
| title_short | Bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in Punjab, India |
| title_sort | bioaccumulation of pesticide contaminants in tissue matrices of dogs suffering from malignant canine mammary tumors in punjab india |
| topic | Cancer research Environmental science Toxicology Veterinary medicine Clinical research Clinical toxicology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020321174 |
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