Increased serum exosomal miR-134 expression in the acute ischemic stroke patients
Abstract Background The exosomal miRNAs have been emerged as biomarkers and therapeutic targets for various diseases, however, the function of exosomal miRNAs in stroke remains largely unknown. Methods The blood samples from acute ischemic stroke (AIS) patients and normal controls were collected. Th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Neurology |
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| Online Access: | http://link.springer.com/article/10.1186/s12883-018-1196-z |
| _version_ | 1811312075106942976 |
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| author | Jingxia Zhou Lin Chen Bocan Chen Shaozhu Huang Chaosheng Zeng Hairong Wu Cong Chen Faqing Long |
| author_facet | Jingxia Zhou Lin Chen Bocan Chen Shaozhu Huang Chaosheng Zeng Hairong Wu Cong Chen Faqing Long |
| author_sort | Jingxia Zhou |
| collection | DOAJ |
| description | Abstract Background The exosomal miRNAs have been emerged as biomarkers and therapeutic targets for various diseases, however, the function of exosomal miRNAs in stroke remains largely unknown. Methods The blood samples from acute ischemic stroke (AIS) patients and normal controls were collected. The exosomes were isolated from the blood samples, which were confirmed by electron microscopy and western blot with the specific exosomes biomarker CD9, CD63 and Tsg101. Results RT-qPCR analysis showed that exosomal miR-134 was significantly increased in AIS patients within 24 h after stroke onset compared with that of control group. Highly expressed exosomal miR-134 was correlated with the National Institutes of Health Stroke Scale (NIHSS) scores, infarct volume and positively associated with the worse prognosis of the stroke patients. Additionally, the exosomal miR-134 was strong positively correlated with the expression of serum interleukin 6 (IL-6) and plasma high-sensitivity C relative protein (hs-CRP). The receiver operating characteristic (ROC) curve suggested that miR-134 might be a potential factor to discriminate AIS patients from non-stroke controls. Conclusions The exosomal miR-134 as a possible novel biomarker for the diagnosis and prognosis of stroke. |
| first_indexed | 2024-04-13T10:29:56Z |
| format | Article |
| id | doaj.art-dfa169a0d651455eb3f90d3dd2199658 |
| institution | Directory Open Access Journal |
| issn | 1471-2377 |
| language | English |
| last_indexed | 2024-04-13T10:29:56Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neurology |
| spelling | doaj.art-dfa169a0d651455eb3f90d3dd21996582022-12-22T02:50:13ZengBMCBMC Neurology1471-23772018-12-011811910.1186/s12883-018-1196-zIncreased serum exosomal miR-134 expression in the acute ischemic stroke patientsJingxia Zhou0Lin Chen1Bocan Chen2Shaozhu Huang3Chaosheng Zeng4Hairong Wu5Cong Chen6Faqing Long7Department of Neurology, The Second Affiliated Hospital of Hainan Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Hainan Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Hainan Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Hainan Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Hainan Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Hainan Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Hainan Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Hainan Medical UniversityAbstract Background The exosomal miRNAs have been emerged as biomarkers and therapeutic targets for various diseases, however, the function of exosomal miRNAs in stroke remains largely unknown. Methods The blood samples from acute ischemic stroke (AIS) patients and normal controls were collected. The exosomes were isolated from the blood samples, which were confirmed by electron microscopy and western blot with the specific exosomes biomarker CD9, CD63 and Tsg101. Results RT-qPCR analysis showed that exosomal miR-134 was significantly increased in AIS patients within 24 h after stroke onset compared with that of control group. Highly expressed exosomal miR-134 was correlated with the National Institutes of Health Stroke Scale (NIHSS) scores, infarct volume and positively associated with the worse prognosis of the stroke patients. Additionally, the exosomal miR-134 was strong positively correlated with the expression of serum interleukin 6 (IL-6) and plasma high-sensitivity C relative protein (hs-CRP). The receiver operating characteristic (ROC) curve suggested that miR-134 might be a potential factor to discriminate AIS patients from non-stroke controls. Conclusions The exosomal miR-134 as a possible novel biomarker for the diagnosis and prognosis of stroke.http://link.springer.com/article/10.1186/s12883-018-1196-zAcute ischemic strokeExosomemiR-134National Institutes of Health stroke scale scoresInfarct volume |
| spellingShingle | Jingxia Zhou Lin Chen Bocan Chen Shaozhu Huang Chaosheng Zeng Hairong Wu Cong Chen Faqing Long Increased serum exosomal miR-134 expression in the acute ischemic stroke patients BMC Neurology Acute ischemic stroke Exosome miR-134 National Institutes of Health stroke scale scores Infarct volume |
| title | Increased serum exosomal miR-134 expression in the acute ischemic stroke patients |
| title_full | Increased serum exosomal miR-134 expression in the acute ischemic stroke patients |
| title_fullStr | Increased serum exosomal miR-134 expression in the acute ischemic stroke patients |
| title_full_unstemmed | Increased serum exosomal miR-134 expression in the acute ischemic stroke patients |
| title_short | Increased serum exosomal miR-134 expression in the acute ischemic stroke patients |
| title_sort | increased serum exosomal mir 134 expression in the acute ischemic stroke patients |
| topic | Acute ischemic stroke Exosome miR-134 National Institutes of Health stroke scale scores Infarct volume |
| url | http://link.springer.com/article/10.1186/s12883-018-1196-z |
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