The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica
ENTERORapid 24 kit (PLIVA-Lachema, Czech Republic) was used for the identification of 321 strains isolated from the respiratory tract of different animal species in the CzechRepublic and Ethiopia. A total of 207 ( 64.5%) strains were identified at the species level within 4 to 8 hours of incubation....
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Format: | Article |
Language: | English |
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Czech Academy of Agricultural Sciences
2002-04-01
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Series: | Veterinární Medicína |
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Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200204-0003_the-utility-of-the-enterorapid-24-kit-for-the-identification-of-p-multocida-and-m-haemolytica.php |
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author | G. Tefera J. Smola |
author_facet | G. Tefera J. Smola |
author_sort | G. Tefera |
collection | DOAJ |
description | ENTERORapid 24 kit (PLIVA-Lachema, Czech Republic) was used for the identification of 321 strains isolated from the respiratory tract of different animal species in the CzechRepublic and Ethiopia. A total of 207 ( 64.5%) strains were identified at the species level within 4 to 8 hours of incubation. In the same way, 39 (12.1%) strains were successfully classified at the genus level. The remaining strains (23.4%) were not identified nor classified to the family Pasteurellaceae. On the other hand, the accuracy of the ENTERORapid 24 kit for the identification of P. multocida and M. haemoyltica was observed using 9 reference strains and the identification results were compaired with the results of the RapiD 20E kit (bioMérieux, France), which required an overall examination time of 4 hours. According to our observation, the ENTERO Rapid 24 kit is the fastest system forthe identification of P. multocida and M. haemolytica strains within 4 to 8 hours with a correct identification rate at thespecies level, with and without additional tests. For these reasons, we propose its modification for rapid identificaton of P. multocida, M. haemolytica and related bacterial species from the family Pasteurellaceae isolated from different animal species. |
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issn | 0375-8427 1805-9392 |
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series | Veterinární Medicína |
spelling | doaj.art-dfa239a2bd3d409ea896d893849c89162023-02-23T03:48:59ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922002-04-014749910310.17221/5811-VETMEDvet-200204-0003The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolyticaG. Tefera0J. Smola1Institute of Microbiology and Immunology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicInstitute of Microbiology and Immunology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicENTERORapid 24 kit (PLIVA-Lachema, Czech Republic) was used for the identification of 321 strains isolated from the respiratory tract of different animal species in the CzechRepublic and Ethiopia. A total of 207 ( 64.5%) strains were identified at the species level within 4 to 8 hours of incubation. In the same way, 39 (12.1%) strains were successfully classified at the genus level. The remaining strains (23.4%) were not identified nor classified to the family Pasteurellaceae. On the other hand, the accuracy of the ENTERORapid 24 kit for the identification of P. multocida and M. haemoyltica was observed using 9 reference strains and the identification results were compaired with the results of the RapiD 20E kit (bioMérieux, France), which required an overall examination time of 4 hours. According to our observation, the ENTERO Rapid 24 kit is the fastest system forthe identification of P. multocida and M. haemolytica strains within 4 to 8 hours with a correct identification rate at thespecies level, with and without additional tests. For these reasons, we propose its modification for rapid identificaton of P. multocida, M. haemolytica and related bacterial species from the family Pasteurellaceae isolated from different animal species.https://vetmed.agriculturejournals.cz/artkey/vet-200204-0003_the-utility-of-the-enterorapid-24-kit-for-the-identification-of-p-multocida-and-m-haemolytica.phpp. multocidam. haemolyticapasteurellaceaerapid identificationcommercial kits |
spellingShingle | G. Tefera J. Smola The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica Veterinární Medicína p. multocida m. haemolytica pasteurellaceae rapid identification commercial kits |
title | The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica |
title_full | The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica |
title_fullStr | The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica |
title_full_unstemmed | The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica |
title_short | The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica |
title_sort | utility of the enterorapid 24 kit for the identification of p multocida and m haemolytica |
topic | p. multocida m. haemolytica pasteurellaceae rapid identification commercial kits |
url | https://vetmed.agriculturejournals.cz/artkey/vet-200204-0003_the-utility-of-the-enterorapid-24-kit-for-the-identification-of-p-multocida-and-m-haemolytica.php |
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