The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica

ENTERORapid 24 kit (PLIVA-Lachema, Czech Republic) was used for the identification of 321 strains isolated from the respiratory tract of different animal species in the CzechRepublic and Ethiopia. A total of 207 ( 64.5%) strains were identified at the species level within 4 to 8 hours of incubation....

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Main Authors: G. Tefera, J. Smola
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2002-04-01
Series:Veterinární Medicína
Subjects:
Online Access:https://vetmed.agriculturejournals.cz/artkey/vet-200204-0003_the-utility-of-the-enterorapid-24-kit-for-the-identification-of-p-multocida-and-m-haemolytica.php
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author G. Tefera
J. Smola
author_facet G. Tefera
J. Smola
author_sort G. Tefera
collection DOAJ
description ENTERORapid 24 kit (PLIVA-Lachema, Czech Republic) was used for the identification of 321 strains isolated from the respiratory tract of different animal species in the CzechRepublic and Ethiopia. A total of 207 ( 64.5%) strains were identified at the species level within 4 to 8 hours of incubation. In the same way, 39 (12.1%) strains were successfully classified at the genus level. The remaining strains (23.4%) were not identified nor classified to the family Pasteurellaceae. On the other hand, the accuracy of the ENTERORapid 24 kit for the identification of P. multocida and M. haemoyltica was observed using 9 reference strains and the identification results were compaired with the results of the RapiD 20E kit (bioMérieux, France), which required an overall examination time of 4 hours. According to our observation, the ENTERO Rapid 24 kit is the fastest system forthe identification of P. multocida and M. haemolytica strains within 4 to 8 hours with a correct identification rate at thespecies level, with and without additional tests. For these reasons, we propose its modification for rapid identificaton of P. multocida, M. haemolytica and related bacterial species from the family Pasteurellaceae isolated from different animal species.
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spelling doaj.art-dfa239a2bd3d409ea896d893849c89162023-02-23T03:48:59ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922002-04-014749910310.17221/5811-VETMEDvet-200204-0003The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolyticaG. Tefera0J. Smola1Institute of Microbiology and Immunology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicInstitute of Microbiology and Immunology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicENTERORapid 24 kit (PLIVA-Lachema, Czech Republic) was used for the identification of 321 strains isolated from the respiratory tract of different animal species in the CzechRepublic and Ethiopia. A total of 207 ( 64.5%) strains were identified at the species level within 4 to 8 hours of incubation. In the same way, 39 (12.1%) strains were successfully classified at the genus level. The remaining strains (23.4%) were not identified nor classified to the family Pasteurellaceae. On the other hand, the accuracy of the ENTERORapid 24 kit for the identification of P. multocida and M. haemoyltica was observed using 9 reference strains and the identification results were compaired with the results of the RapiD 20E kit (bioMérieux, France), which required an overall examination time of 4 hours. According to our observation, the ENTERO Rapid 24 kit is the fastest system forthe identification of P. multocida and M. haemolytica strains within 4 to 8 hours with a correct identification rate at thespecies level, with and without additional tests. For these reasons, we propose its modification for rapid identificaton of P. multocida, M. haemolytica and related bacterial species from the family Pasteurellaceae isolated from different animal species.https://vetmed.agriculturejournals.cz/artkey/vet-200204-0003_the-utility-of-the-enterorapid-24-kit-for-the-identification-of-p-multocida-and-m-haemolytica.phpp. multocidam. haemolyticapasteurellaceaerapid identificationcommercial kits
spellingShingle G. Tefera
J. Smola
The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica
Veterinární Medicína
p. multocida
m. haemolytica
pasteurellaceae
rapid identification
commercial kits
title The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica
title_full The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica
title_fullStr The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica
title_full_unstemmed The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica
title_short The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica
title_sort utility of the enterorapid 24 kit for the identification of p multocida and m haemolytica
topic p. multocida
m. haemolytica
pasteurellaceae
rapid identification
commercial kits
url https://vetmed.agriculturejournals.cz/artkey/vet-200204-0003_the-utility-of-the-enterorapid-24-kit-for-the-identification-of-p-multocida-and-m-haemolytica.php
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