Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C Mice
Background and objectives: Astragalus spp., have been used as a pain reliever in traditional medicine; therefore, in this study, we decided to evaluate the antinociceptive effects of Astragalus ecbatanus chloroform extract (AECE) in acute and chronic pain in male mice. Methods: The extract was obtai...
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| Format: | Article |
| Language: | English |
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Iranian Society of Pharmacognosy
2023-04-01
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| Series: | Research Journal of Pharmacognosy |
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| Online Access: | http://www.rjpharmacognosy.ir/article_168646_99d7644d339f567ba7351efa52347df9.pdf |
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| author | Javad Ghasemian Yadegari Ezatollah Fazeli Moghadam Hazhir Golmohammadi Setareh Dastyarhaghighi Mehrdad Ghoulami Hossein Mahmoudvand* |
| author_facet | Javad Ghasemian Yadegari Ezatollah Fazeli Moghadam Hazhir Golmohammadi Setareh Dastyarhaghighi Mehrdad Ghoulami Hossein Mahmoudvand* |
| author_sort | Javad Ghasemian Yadegari |
| collection | DOAJ |
| description | Background and objectives: Astragalus spp., have been used as a pain reliever in traditional medicine; therefore, in this study, we decided to evaluate the antinociceptive effects of Astragalus ecbatanus chloroform extract (AECE) in acute and chronic pain in male mice. Methods: The extract was obtained from aerial parts of A. ecbatanus with maceration method. The antinociceptive effect of AECE was determined by tail-flick, hot-plate, formalin, and rotarod tests followed by the oral intake of mice with AECE at the doses of 200, 400, and 800 mg/kg for 14 days in male Balb/C mice. Results: The results showed AECE at the concentrations of 400 and 800 mg/kg revealed a mean latency time of 6.4 and 7.2 s, respectively; representing a remarkable (p<0.05) antinociceptive activity compared with the control group. AECE, especially at the doses of 400 and 800 mg/kg, significantly increased the time until the occurrence of painful behaviors (licking or jumping) compared to the control group (p<0.001). The results showed AECE, especially in concentrations of 400 and 800 mg/kg, markedly (p<0.05) reduced the pain behaviors in the first phase (acute) and the second (chronic) phase of the formalin test compared to the control group. Conclusion: According to the reducing pain effect of this plant in both pain tests and in both stages of the formalin test, it can be concluded that Astragalus ecbatanus reduces both acute pain and chronic pain, and can relieve pain both peripherally and centrally. |
| first_indexed | 2024-04-09T19:33:55Z |
| format | Article |
| id | doaj.art-dfa4e259bc584befa18b3082d777e887 |
| institution | Directory Open Access Journal |
| issn | 2345-4458 2345-5977 |
| language | English |
| last_indexed | 2024-04-09T19:33:55Z |
| publishDate | 2023-04-01 |
| publisher | Iranian Society of Pharmacognosy |
| record_format | Article |
| series | Research Journal of Pharmacognosy |
| spelling | doaj.art-dfa4e259bc584befa18b3082d777e8872023-04-04T20:51:41ZengIranian Society of PharmacognosyResearch Journal of Pharmacognosy2345-44582345-59772023-04-01102394510.22127/rjp.2023.369364.2007168646Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C MiceJavad Ghasemian Yadegari0Ezatollah Fazeli Moghadam1Hazhir Golmohammadi2Setareh Dastyarhaghighi3Mehrdad Ghoulami4Hossein Mahmoudvand*5Department of Pharmacognosy, Lorestan University of Medical Sciences, Khorramabad, Iran.Department of Nutrition, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran.Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Background and objectives: Astragalus spp., have been used as a pain reliever in traditional medicine; therefore, in this study, we decided to evaluate the antinociceptive effects of Astragalus ecbatanus chloroform extract (AECE) in acute and chronic pain in male mice. Methods: The extract was obtained from aerial parts of A. ecbatanus with maceration method. The antinociceptive effect of AECE was determined by tail-flick, hot-plate, formalin, and rotarod tests followed by the oral intake of mice with AECE at the doses of 200, 400, and 800 mg/kg for 14 days in male Balb/C mice. Results: The results showed AECE at the concentrations of 400 and 800 mg/kg revealed a mean latency time of 6.4 and 7.2 s, respectively; representing a remarkable (p<0.05) antinociceptive activity compared with the control group. AECE, especially at the doses of 400 and 800 mg/kg, significantly increased the time until the occurrence of painful behaviors (licking or jumping) compared to the control group (p<0.001). The results showed AECE, especially in concentrations of 400 and 800 mg/kg, markedly (p<0.05) reduced the pain behaviors in the first phase (acute) and the second (chronic) phase of the formalin test compared to the control group. Conclusion: According to the reducing pain effect of this plant in both pain tests and in both stages of the formalin test, it can be concluded that Astragalus ecbatanus reduces both acute pain and chronic pain, and can relieve pain both peripherally and centrally.http://www.rjpharmacognosy.ir/article_168646_99d7644d339f567ba7351efa52347df9.pdfastragalus ecbatanusextractherbmicepain |
| spellingShingle | Javad Ghasemian Yadegari Ezatollah Fazeli Moghadam Hazhir Golmohammadi Setareh Dastyarhaghighi Mehrdad Ghoulami Hossein Mahmoudvand* Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C Mice Research Journal of Pharmacognosy astragalus ecbatanus extract herb mice pain |
| title | Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C Mice |
| title_full | Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C Mice |
| title_fullStr | Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C Mice |
| title_full_unstemmed | Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C Mice |
| title_short | Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C Mice |
| title_sort | effect of oral administration of astragalus ecbatanus chloroform extract on acute and chronic pain in balb c mice |
| topic | astragalus ecbatanus extract herb mice pain |
| url | http://www.rjpharmacognosy.ir/article_168646_99d7644d339f567ba7351efa52347df9.pdf |
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