Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis
In this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/8/264 |
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| author | Chunxue Wang Pengfei Yi Jiang Li Haibing Dong Changming Chen Daming Zhang Haiqing Shen Bendong Fu |
| author_facet | Chunxue Wang Pengfei Yi Jiang Li Haibing Dong Changming Chen Daming Zhang Haiqing Shen Bendong Fu |
| author_sort | Chunxue Wang |
| collection | DOAJ |
| description | In this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8 (FSU-8) as the sensing core layer and polymethyl methacrylate (PMMA) as the sensing window cladding were synthesized. The effective drug concentration range (5–10 µg/mL) of ginkgolide A for inhibition of PMVEC apoptosis was analyzed and obtained by pharmacological studies. The structure of the device was optimized to be designed and fabricated by direct UV writing technology. The properties of the biosensor were simulated with various refractive indices of different drug concentrations. The actual sensitivity of the biosensor was measured as 1606.2 nm/RIU. The resolution and detection limit were characterized as 0.05 nm and 3 × 10<sup>−5</sup> RIU, respectively. The technique is suitable for safe and accurate detection of effective organic drug dosages of Chinese herbal ingredients. |
| first_indexed | 2024-03-10T08:58:52Z |
| format | Article |
| id | doaj.art-dfa6d25ebb7845f2a4b997ae973b50f0 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T08:58:52Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-dfa6d25ebb7845f2a4b997ae973b50f02023-11-22T06:57:33ZengMDPI AGBiosensors2079-63742021-08-0111826410.3390/bios11080264Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC ApoptosisChunxue Wang0Pengfei Yi1Jiang Li2Haibing Dong3Changming Chen4Daming Zhang5Haiqing Shen6Bendong Fu7College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, ChinaState Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaIn this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8 (FSU-8) as the sensing core layer and polymethyl methacrylate (PMMA) as the sensing window cladding were synthesized. The effective drug concentration range (5–10 µg/mL) of ginkgolide A for inhibition of PMVEC apoptosis was analyzed and obtained by pharmacological studies. The structure of the device was optimized to be designed and fabricated by direct UV writing technology. The properties of the biosensor were simulated with various refractive indices of different drug concentrations. The actual sensitivity of the biosensor was measured as 1606.2 nm/RIU. The resolution and detection limit were characterized as 0.05 nm and 3 × 10<sup>−5</sup> RIU, respectively. The technique is suitable for safe and accurate detection of effective organic drug dosages of Chinese herbal ingredients.https://www.mdpi.com/2079-6374/11/8/264waveguide grating-based biosensorpolymer optical waveguideeffective drug concentrations |
| spellingShingle | Chunxue Wang Pengfei Yi Jiang Li Haibing Dong Changming Chen Daming Zhang Haiqing Shen Bendong Fu Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis Biosensors waveguide grating-based biosensor polymer optical waveguide effective drug concentrations |
| title | Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis |
| title_full | Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis |
| title_fullStr | Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis |
| title_full_unstemmed | Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis |
| title_short | Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis |
| title_sort | polymer optical waveguide grating based biosensor to detect effective drug concentrations of ginkgolide a for inhibition of pmvec apoptosis |
| topic | waveguide grating-based biosensor polymer optical waveguide effective drug concentrations |
| url | https://www.mdpi.com/2079-6374/11/8/264 |
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