The beneficial effect of nanomethionine supplementation on growth performance, gene expression profile, and histopathology of heat-stressed broiler chicken
ABSTRACT: This study investigated the effects of nanomethionine (nano-meth) on performance, antioxidants, and gene expression of HSP70, HSP90 and Heat Shock factor-1 (HSF-1) from the liver, and TLR4 from the jejunum, of broiler chickens reared under normal temperatures or under heat stress. Three hu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123007253 |
| _version_ | 1797366692697866240 |
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| author | Set A. El-Shobokshy Magda I. Abo-Samaha Asmaa F. Khafaga Khloud G. Fakiha Shymaa A. Khatab Eman Mohammed Abdelmaksoud Mosaad Abdel khalek Soltan Sarah I. Othman Hassan A. Rudayni Ahmed A. Allam Mohamed Emam |
| author_facet | Set A. El-Shobokshy Magda I. Abo-Samaha Asmaa F. Khafaga Khloud G. Fakiha Shymaa A. Khatab Eman Mohammed Abdelmaksoud Mosaad Abdel khalek Soltan Sarah I. Othman Hassan A. Rudayni Ahmed A. Allam Mohamed Emam |
| author_sort | Set A. El-Shobokshy |
| collection | DOAJ |
| description | ABSTRACT: This study investigated the effects of nanomethionine (nano-meth) on performance, antioxidants, and gene expression of HSP70, HSP90 and Heat Shock factor-1 (HSF-1) from the liver, and TLR4 from the jejunum, of broiler chickens reared under normal temperatures or under heat stress. Three hundred 1-day-old chicks were randomly assigned to 5 treatment groups. Group 1 served as control. Under normal temperature, birds in group 2 received nano-meth (10 mL/L of drinking water) from d1 until the experiment ended. Group 3 birds were heat-stressed (HS) and did not receive any supplementation. Group 4 received nano-meth in the same dose from d1 old until experiment ended, and the birds were exposed to HS. Group 5 birds were HS and received supplementation of nano-meth during the HS period only. Nano-meth improved (P < 0.0001) final body weight, weight gain, feed conversion ratio, and also decreased (P < 0.0001) the effect of HS on growth performance. Reduction (P < 0.0001) in malondialdehyde and changes in antioxidant enzymes GPX and CAT activity indicated the antioxidant effect of nano-meth. Nano-meth supplementation caused an increase in the expression of HSP70 , HSP90 and HSF1, and a downregulation of TLR4 gene expression. Additionally, nano-meth-supplemented groups showed marked improvement in the histological liver structure, intestinal morphology and villus height compared to control or HS groups. |
| first_indexed | 2024-03-08T17:08:03Z |
| format | Article |
| id | doaj.art-dfa8c4a9f1d649beab5a45d82014e56f |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-08T17:08:03Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-dfa8c4a9f1d649beab5a45d82014e56f2024-01-04T04:35:38ZengElsevierPoultry Science0032-57912024-01-011031103206The beneficial effect of nanomethionine supplementation on growth performance, gene expression profile, and histopathology of heat-stressed broiler chickenSet A. El-Shobokshy0Magda I. Abo-Samaha1Asmaa F. Khafaga2Khloud G. Fakiha3Shymaa A. Khatab4Eman Mohammed Abdelmaksoud5Mosaad Abdel khalek Soltan6Sarah I. Othman7Hassan A. Rudayni8Ahmed A. Allam9Mohamed Emam10Department of Nutrition and Veterinary Clinical Nutrition, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, EgyptPoultry Breeding and Production, Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, Egypt; Corresponding author:Department of Biology, College of Science, University of Jeddah, Jeddah, Saudi ArabiaGenetics and Genetic Engineering, Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, EgyptDepartment of Nutrition and Veterinary Clinical Nutrition, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi ArabiaDepartment of Biology, College of Science, Imam Muhammad bin Saud Islamic University, Riyadh 11623, Saudi ArabiaDepartment of Zoology, Faculty of Science, Beni-suef University, Beni-suef 65211, EgyptDepartment of Nutrition and Veterinary Clinical Nutrition, Damanhour University, Damanhour 22511, EgyptABSTRACT: This study investigated the effects of nanomethionine (nano-meth) on performance, antioxidants, and gene expression of HSP70, HSP90 and Heat Shock factor-1 (HSF-1) from the liver, and TLR4 from the jejunum, of broiler chickens reared under normal temperatures or under heat stress. Three hundred 1-day-old chicks were randomly assigned to 5 treatment groups. Group 1 served as control. Under normal temperature, birds in group 2 received nano-meth (10 mL/L of drinking water) from d1 until the experiment ended. Group 3 birds were heat-stressed (HS) and did not receive any supplementation. Group 4 received nano-meth in the same dose from d1 old until experiment ended, and the birds were exposed to HS. Group 5 birds were HS and received supplementation of nano-meth during the HS period only. Nano-meth improved (P < 0.0001) final body weight, weight gain, feed conversion ratio, and also decreased (P < 0.0001) the effect of HS on growth performance. Reduction (P < 0.0001) in malondialdehyde and changes in antioxidant enzymes GPX and CAT activity indicated the antioxidant effect of nano-meth. Nano-meth supplementation caused an increase in the expression of HSP70 , HSP90 and HSF1, and a downregulation of TLR4 gene expression. Additionally, nano-meth-supplemented groups showed marked improvement in the histological liver structure, intestinal morphology and villus height compared to control or HS groups.http://www.sciencedirect.com/science/article/pii/S0032579123007253broilernanomethioninegrowth performancegene expressionhistopathology |
| spellingShingle | Set A. El-Shobokshy Magda I. Abo-Samaha Asmaa F. Khafaga Khloud G. Fakiha Shymaa A. Khatab Eman Mohammed Abdelmaksoud Mosaad Abdel khalek Soltan Sarah I. Othman Hassan A. Rudayni Ahmed A. Allam Mohamed Emam The beneficial effect of nanomethionine supplementation on growth performance, gene expression profile, and histopathology of heat-stressed broiler chicken Poultry Science broiler nanomethionine growth performance gene expression histopathology |
| title | The beneficial effect of nanomethionine supplementation on growth performance, gene expression profile, and histopathology of heat-stressed broiler chicken |
| title_full | The beneficial effect of nanomethionine supplementation on growth performance, gene expression profile, and histopathology of heat-stressed broiler chicken |
| title_fullStr | The beneficial effect of nanomethionine supplementation on growth performance, gene expression profile, and histopathology of heat-stressed broiler chicken |
| title_full_unstemmed | The beneficial effect of nanomethionine supplementation on growth performance, gene expression profile, and histopathology of heat-stressed broiler chicken |
| title_short | The beneficial effect of nanomethionine supplementation on growth performance, gene expression profile, and histopathology of heat-stressed broiler chicken |
| title_sort | beneficial effect of nanomethionine supplementation on growth performance gene expression profile and histopathology of heat stressed broiler chicken |
| topic | broiler nanomethionine growth performance gene expression histopathology |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123007253 |
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