Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production

Yeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research wa...

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Main Authors: EFRIWATI, ANTONIUS SUWANTO, GAYUH RAHAYU, LILIS NURAIDA
Format: Article
Language:English
Published: Bogor Agricultural University 2013-06-01
Series:Hayati Journal of Biosciences
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1978301916301048
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author EFRIWATI
ANTONIUS SUWANTO
GAYUH RAHAYU
LILIS NURAIDA
author_facet EFRIWATI
ANTONIUS SUWANTO
GAYUH RAHAYU
LILIS NURAIDA
author_sort EFRIWATI
collection DOAJ
description Yeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research was aimed in studying the effect of different methods of tempeh production applied in tempeh home industry on the dynamics of yeast and LAB communities. Population dynamics was expressed as both changes of colony number and its phylotype. Samples were obtained from five stages and from two different methods of tempeh production. Observations were carried out employing colony counting on selective media followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The study indicated that the population of yeasts and LAB during tempeh production were dynamic and different between these methods. Tempeh production methods affected the presence of yeasts and LAB population as indicated by difference in colony number, the number and diversity of phylotype, as well as number of specific phylotypes grew on plates.
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spelling doaj.art-dfa90b9e398d4cde95701004f12aeaa92022-12-22T00:02:10ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192013-06-01202576410.4308/hjb.20.2.57Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh ProductionEFRIWATI0ANTONIUS SUWANTO1GAYUH RAHAYU2LILIS NURAIDA3Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaDepartment Food Science and Technology, Faculty of Agricultural Technology, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaYeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research was aimed in studying the effect of different methods of tempeh production applied in tempeh home industry on the dynamics of yeast and LAB communities. Population dynamics was expressed as both changes of colony number and its phylotype. Samples were obtained from five stages and from two different methods of tempeh production. Observations were carried out employing colony counting on selective media followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The study indicated that the population of yeasts and LAB during tempeh production were dynamic and different between these methods. Tempeh production methods affected the presence of yeasts and LAB population as indicated by difference in colony number, the number and diversity of phylotype, as well as number of specific phylotypes grew on plates.http://www.sciencedirect.com/science/article/pii/S1978301916301048tempehT-RFLPyeastlactic acid bacteria
spellingShingle EFRIWATI
ANTONIUS SUWANTO
GAYUH RAHAYU
LILIS NURAIDA
Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production
Hayati Journal of Biosciences
tempeh
T-RFLP
yeast
lactic acid bacteria
title Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production
title_full Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production
title_fullStr Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production
title_full_unstemmed Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production
title_short Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production
title_sort population dynamics of yeasts and lactic acid bacteria lab during tempeh production
topic tempeh
T-RFLP
yeast
lactic acid bacteria
url http://www.sciencedirect.com/science/article/pii/S1978301916301048
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AT antoniussuwanto populationdynamicsofyeastsandlacticacidbacterialabduringtempehproduction
AT gayuhrahayu populationdynamicsofyeastsandlacticacidbacterialabduringtempehproduction
AT lilisnuraida populationdynamicsofyeastsandlacticacidbacterialabduringtempehproduction