Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production
Yeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research wa...
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Format: | Article |
Language: | English |
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Bogor Agricultural University
2013-06-01
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Series: | Hayati Journal of Biosciences |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916301048 |
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author | EFRIWATI ANTONIUS SUWANTO GAYUH RAHAYU LILIS NURAIDA |
author_facet | EFRIWATI ANTONIUS SUWANTO GAYUH RAHAYU LILIS NURAIDA |
author_sort | EFRIWATI |
collection | DOAJ |
description | Yeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research was aimed in studying the effect of different methods of tempeh production applied in tempeh home industry on the dynamics of yeast and LAB communities. Population dynamics was expressed as both changes of colony number and its phylotype. Samples were obtained from five stages and from two different methods of tempeh production. Observations were carried out employing colony counting on selective media followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The study indicated that the population of yeasts and LAB during tempeh production were dynamic and different between these methods. Tempeh production methods affected the presence of yeasts and LAB population as indicated by difference in colony number, the number and diversity of phylotype, as well as number of specific phylotypes grew on plates. |
first_indexed | 2024-12-13T02:47:14Z |
format | Article |
id | doaj.art-dfa90b9e398d4cde95701004f12aeaa9 |
institution | Directory Open Access Journal |
issn | 1978-3019 |
language | English |
last_indexed | 2024-12-13T02:47:14Z |
publishDate | 2013-06-01 |
publisher | Bogor Agricultural University |
record_format | Article |
series | Hayati Journal of Biosciences |
spelling | doaj.art-dfa90b9e398d4cde95701004f12aeaa92022-12-22T00:02:10ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192013-06-01202576410.4308/hjb.20.2.57Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh ProductionEFRIWATI0ANTONIUS SUWANTO1GAYUH RAHAYU2LILIS NURAIDA3Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaDepartment Food Science and Technology, Faculty of Agricultural Technology, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaYeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research was aimed in studying the effect of different methods of tempeh production applied in tempeh home industry on the dynamics of yeast and LAB communities. Population dynamics was expressed as both changes of colony number and its phylotype. Samples were obtained from five stages and from two different methods of tempeh production. Observations were carried out employing colony counting on selective media followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The study indicated that the population of yeasts and LAB during tempeh production were dynamic and different between these methods. Tempeh production methods affected the presence of yeasts and LAB population as indicated by difference in colony number, the number and diversity of phylotype, as well as number of specific phylotypes grew on plates.http://www.sciencedirect.com/science/article/pii/S1978301916301048tempehT-RFLPyeastlactic acid bacteria |
spellingShingle | EFRIWATI ANTONIUS SUWANTO GAYUH RAHAYU LILIS NURAIDA Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production Hayati Journal of Biosciences tempeh T-RFLP yeast lactic acid bacteria |
title | Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production |
title_full | Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production |
title_fullStr | Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production |
title_full_unstemmed | Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production |
title_short | Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production |
title_sort | population dynamics of yeasts and lactic acid bacteria lab during tempeh production |
topic | tempeh T-RFLP yeast lactic acid bacteria |
url | http://www.sciencedirect.com/science/article/pii/S1978301916301048 |
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