Effects of dietary β-glucan on growth rate, antioxidant status, immune response, and resistance against Aeromonas hydrophila in genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus)
This study was conducted to assess the influences of dietary β-glucan on growth rate, antioxidant status, immunity response, and resistance against Aeromonas hydrophila of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Four isonitrogenous and isoenergetic diets were formulated to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423000194 |
| _version_ | 1827981849750667264 |
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| author | Xiaomei Dou Han Huang Ying Li Junming Deng Beiping Tan |
| author_facet | Xiaomei Dou Han Huang Ying Li Junming Deng Beiping Tan |
| author_sort | Xiaomei Dou |
| collection | DOAJ |
| description | This study was conducted to assess the influences of dietary β-glucan on growth rate, antioxidant status, immunity response, and resistance against Aeromonas hydrophila of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Four isonitrogenous and isoenergetic diets were formulated to contain 0%, 0.2%, 0.4%, and 0.8% β-glucan, respectively. Fish with similar size (average body weight, 3.43 ± 0.02 g) were randomly distributed into 12 tanks with 40 juveniles per tank and hand-fed to apparent satiation twice daily for 10 weeks. The fish were then challenged by A. hydrophila and their cumulative survival rate was recorded for the next week. No obvious influences on growth rate and feed utilization of GIFT were induced by dietary β-glucan addition (P > 0.05). However, dietary 0.4% β-glucan inclusion increased the serum high-density lipoprotein cholesterol level but decreased the triglyceride, total cholesterol and low-density lipoprotein cholesterol levels as well as alanine aminotransferase and aspartate aminotransferase activities (P < 0.05). Similarly, the increased intestinal superoxide dismutase (SOD), peroxidase and total antioxidant capacity (TAC) activities as well as the decreased intestinal malondialdehyde (MDA) and reactive oxygen species contents were also observed in GIFT fed diet with 0.4% β-glucan (P < 0.05). Dietary addition of 0.8% β-glucan increased the plasma SOD, glutathione peroxidase (GPx), catalase, and TAC activities, but reduced the plasma MDA content (P < 0.05). Dietary β-glucan inclusion regardless of levels improved the hepatic SOD, glutathione reductase, catalase, peroxidase, TAC, and acid phosphatase activities, but depressed the hepatic nitric oxide and MDA contents (P < 0.05). Plasma lysozyme activity was improved by the inclusion of 0.4% and 0.8% β-glucan (P < 0.05); plasma immunoglobulin M, complement 3 and complement 4 contents were improved by dietary β-glucan inclusion (P < 0.05); respiratory burst activity of macrophages was improved by the addition of 0.2% β-glucan (P < 0.05). Additionally, dietary β-glucan supplementation reduced the mortality rate and increased the relative percentage survival of GIFT challenged by A. hydrophila (P < 0.05). These results indicated that suitable β-glucan inclusion (0.4–0.8%) could promote the antioxidant status, immune response and disease resistance of GIFT, and β-glucan can be used as a feed additive for GIFT. |
| first_indexed | 2024-04-09T22:15:35Z |
| format | Article |
| id | doaj.art-dfa9a694c1dd4d5cac00d93c1a531806 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-09T22:15:35Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-dfa9a694c1dd4d5cac00d93c1a5318062023-03-23T04:36:17ZengElsevierAquaculture Reports2352-51342023-04-0129101480Effects of dietary β-glucan on growth rate, antioxidant status, immune response, and resistance against Aeromonas hydrophila in genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus)Xiaomei Dou0Han Huang1Ying Li2Junming Deng3Beiping Tan4College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China; Correspondence to: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Correspondence to: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.This study was conducted to assess the influences of dietary β-glucan on growth rate, antioxidant status, immunity response, and resistance against Aeromonas hydrophila of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Four isonitrogenous and isoenergetic diets were formulated to contain 0%, 0.2%, 0.4%, and 0.8% β-glucan, respectively. Fish with similar size (average body weight, 3.43 ± 0.02 g) were randomly distributed into 12 tanks with 40 juveniles per tank and hand-fed to apparent satiation twice daily for 10 weeks. The fish were then challenged by A. hydrophila and their cumulative survival rate was recorded for the next week. No obvious influences on growth rate and feed utilization of GIFT were induced by dietary β-glucan addition (P > 0.05). However, dietary 0.4% β-glucan inclusion increased the serum high-density lipoprotein cholesterol level but decreased the triglyceride, total cholesterol and low-density lipoprotein cholesterol levels as well as alanine aminotransferase and aspartate aminotransferase activities (P < 0.05). Similarly, the increased intestinal superoxide dismutase (SOD), peroxidase and total antioxidant capacity (TAC) activities as well as the decreased intestinal malondialdehyde (MDA) and reactive oxygen species contents were also observed in GIFT fed diet with 0.4% β-glucan (P < 0.05). Dietary addition of 0.8% β-glucan increased the plasma SOD, glutathione peroxidase (GPx), catalase, and TAC activities, but reduced the plasma MDA content (P < 0.05). Dietary β-glucan inclusion regardless of levels improved the hepatic SOD, glutathione reductase, catalase, peroxidase, TAC, and acid phosphatase activities, but depressed the hepatic nitric oxide and MDA contents (P < 0.05). Plasma lysozyme activity was improved by the inclusion of 0.4% and 0.8% β-glucan (P < 0.05); plasma immunoglobulin M, complement 3 and complement 4 contents were improved by dietary β-glucan inclusion (P < 0.05); respiratory burst activity of macrophages was improved by the addition of 0.2% β-glucan (P < 0.05). Additionally, dietary β-glucan supplementation reduced the mortality rate and increased the relative percentage survival of GIFT challenged by A. hydrophila (P < 0.05). These results indicated that suitable β-glucan inclusion (0.4–0.8%) could promote the antioxidant status, immune response and disease resistance of GIFT, and β-glucan can be used as a feed additive for GIFT.http://www.sciencedirect.com/science/article/pii/S2352513423000194Feed additiveHematological parametersLipoproteinRelative percentage survival |
| spellingShingle | Xiaomei Dou Han Huang Ying Li Junming Deng Beiping Tan Effects of dietary β-glucan on growth rate, antioxidant status, immune response, and resistance against Aeromonas hydrophila in genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus) Aquaculture Reports Feed additive Hematological parameters Lipoprotein Relative percentage survival |
| title | Effects of dietary β-glucan on growth rate, antioxidant status, immune response, and resistance against Aeromonas hydrophila in genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus) |
| title_full | Effects of dietary β-glucan on growth rate, antioxidant status, immune response, and resistance against Aeromonas hydrophila in genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus) |
| title_fullStr | Effects of dietary β-glucan on growth rate, antioxidant status, immune response, and resistance against Aeromonas hydrophila in genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus) |
| title_full_unstemmed | Effects of dietary β-glucan on growth rate, antioxidant status, immune response, and resistance against Aeromonas hydrophila in genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus) |
| title_short | Effects of dietary β-glucan on growth rate, antioxidant status, immune response, and resistance against Aeromonas hydrophila in genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus) |
| title_sort | effects of dietary β glucan on growth rate antioxidant status immune response and resistance against aeromonas hydrophila in genetic improvement of farmed tilapia gift oreochromis niloticus |
| topic | Feed additive Hematological parameters Lipoprotein Relative percentage survival |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423000194 |
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