Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in <i>Physalis angulata</i>
The <i>Physalis</i> genus has long been used as traditional medicine in the treatment of various diseases. Physalins, the characteristic class of compounds in this genus, are major bioactive constituents. To date, the biogenesis of physalins remains largely unknown, except for the recent...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/14077 |
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| author | Congkun Hua Zhengqin Xu Nan Tang Yehan Xu Yansheng Zhang Changfu Li |
| author_facet | Congkun Hua Zhengqin Xu Nan Tang Yehan Xu Yansheng Zhang Changfu Li |
| author_sort | Congkun Hua |
| collection | DOAJ |
| description | The <i>Physalis</i> genus has long been used as traditional medicine in the treatment of various diseases. Physalins, the characteristic class of compounds in this genus, are major bioactive constituents. To date, the biogenesis of physalins remains largely unknown, except for the recently established knowledge that 24-methyldesmosterol is a precursor of physalin. To identify the genes encoding P450s that are putatively involved in converting 24-methyldesmosterol to physalins, a total of 306 P450-encoding unigenes were retrieved from our recently constructed <i>P. angulata</i> transcriptome. Extensive phylogenetic analysis proposed 21 P450s that might participate in physalin biosynthesis. To validate the candidates, we developed a virus-induced gene silencing (VIGS) system for <i>P. angulata</i>, and four P450 candidates were selected for the VIGS experiments. The reduction in the transcripts of the four P450 candidates by VIGS all led to decreased levels of physalin-class compounds in the <i>P. angulata</i> leaves. Thus, this study provides a number of P450 candidates that are likely associated with the biosynthesis of physalin-class compounds, forming a strong basis to reveal the unknown physalin biosynthetic pathway in the future. |
| first_indexed | 2024-03-10T22:40:42Z |
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| id | doaj.art-dfab05cde22e41a8ac29471d6aebb3f2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:42Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dfab05cde22e41a8ac29471d6aebb3f22023-11-19T11:07:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181407710.3390/ijms241814077Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in <i>Physalis angulata</i>Congkun Hua0Zhengqin Xu1Nan Tang2Yehan Xu3Yansheng Zhang4Changfu Li5Shanghai Key Laboratory of Bio-Energy Crops, Research Center for Natural Products, Plant Science Center, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaShanghai Key Laboratory of Bio-Energy Crops, Research Center for Natural Products, Plant Science Center, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaShanghai Key Laboratory of Bio-Energy Crops, Research Center for Natural Products, Plant Science Center, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaShanghai Key Laboratory of Bio-Energy Crops, Research Center for Natural Products, Plant Science Center, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaShanghai Key Laboratory of Bio-Energy Crops, Research Center for Natural Products, Plant Science Center, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaShanghai Key Laboratory of Bio-Energy Crops, Research Center for Natural Products, Plant Science Center, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaThe <i>Physalis</i> genus has long been used as traditional medicine in the treatment of various diseases. Physalins, the characteristic class of compounds in this genus, are major bioactive constituents. To date, the biogenesis of physalins remains largely unknown, except for the recently established knowledge that 24-methyldesmosterol is a precursor of physalin. To identify the genes encoding P450s that are putatively involved in converting 24-methyldesmosterol to physalins, a total of 306 P450-encoding unigenes were retrieved from our recently constructed <i>P. angulata</i> transcriptome. Extensive phylogenetic analysis proposed 21 P450s that might participate in physalin biosynthesis. To validate the candidates, we developed a virus-induced gene silencing (VIGS) system for <i>P. angulata</i>, and four P450 candidates were selected for the VIGS experiments. The reduction in the transcripts of the four P450 candidates by VIGS all led to decreased levels of physalin-class compounds in the <i>P. angulata</i> leaves. Thus, this study provides a number of P450 candidates that are likely associated with the biosynthesis of physalin-class compounds, forming a strong basis to reveal the unknown physalin biosynthetic pathway in the future.https://www.mdpi.com/1422-0067/24/18/14077<i>Physalis angulata</i>physalinfull-length transcriptomeP450 |
| spellingShingle | Congkun Hua Zhengqin Xu Nan Tang Yehan Xu Yansheng Zhang Changfu Li Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in <i>Physalis angulata</i> International Journal of Molecular Sciences <i>Physalis angulata</i> physalin full-length transcriptome P450 |
| title | Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in <i>Physalis angulata</i> |
| title_full | Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in <i>Physalis angulata</i> |
| title_fullStr | Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in <i>Physalis angulata</i> |
| title_full_unstemmed | Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in <i>Physalis angulata</i> |
| title_short | Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in <i>Physalis angulata</i> |
| title_sort | identification of p450 candidates associated with the biosynthesis of physalin class compounds in i physalis angulata i |
| topic | <i>Physalis angulata</i> physalin full-length transcriptome P450 |
| url | https://www.mdpi.com/1422-0067/24/18/14077 |
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