Eupafolin induces apoptosis and autophagy of breast cancer cells through PI3K/AKT, MAPKs and NF-κB signaling pathways
Abstract Eupafolin is a flavonoid that can be extracted from common sage. Previous studies have reported that Eupafolin has antioxidant, anti-inflammatory and anti-tumor properties. However, no studies have investigated the role of Eupafolin in breast cancer. Herein, we investigated the effect of Eu...
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| Format: | Article |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-00945-9 |
| _version_ | 1818900925973004288 |
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| author | Jiahui Wei Yu Ding Xinmiao Liu Qing Liu Yiran Lu Song He Bao Yuan Jiabao Zhang |
| author_facet | Jiahui Wei Yu Ding Xinmiao Liu Qing Liu Yiran Lu Song He Bao Yuan Jiabao Zhang |
| author_sort | Jiahui Wei |
| collection | DOAJ |
| description | Abstract Eupafolin is a flavonoid that can be extracted from common sage. Previous studies have reported that Eupafolin has antioxidant, anti-inflammatory and anti-tumor properties. However, no studies have investigated the role of Eupafolin in breast cancer. Herein, we investigated the effect of Eupafolin on two human breast cancer cell lines, as well as its potential mechanism of action. Next, the data showed that proliferation, migration and invasion ability of breast cancer cells that were treated with Eupafolin was significantly reduced, while the apoptosis rate was significantly increased. In addition, Eupafolin treatment caused breast cancer cell proliferation to be blocked in the S phase. Moreover, Eupafolin significantly induced autophagy in breast cancer cells, with an increase in the expression of LC3B-II. PI3K/AKT, MAPKs and NF-κB pathways were significantly inhibited by Eupafolin treatment. Additionally, 3-MA (a blocker of autophagosome formation) significantly reduced Eupafolin-induced activation of LC3B-II in breast cancer cells. Furthermore, Eupafolin displayed good in vitro anti-angiogenic activity. Additionally, anti-breast cancer activity of Eupafolin was found to be partially mediated by Cav-1. Moreover, Eupafolin treatment significantly weakened carcinogenesis of MCF-7 cells in nude mice. Therefore, this data provides novel directions on the use of Eupafolin for treatment of breast cancer. |
| first_indexed | 2024-12-19T20:11:37Z |
| format | Article |
| id | doaj.art-dfaf2b306dcb41e5a130748a18410f1b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T20:11:37Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-dfaf2b306dcb41e5a130748a18410f1b2022-12-21T20:07:18ZengNature PortfolioScientific Reports2045-23222021-11-0111111010.1038/s41598-021-00945-9Eupafolin induces apoptosis and autophagy of breast cancer cells through PI3K/AKT, MAPKs and NF-κB signaling pathwaysJiahui Wei0Yu Ding1Xinmiao Liu2Qing Liu3Yiran Lu4Song He5Bao Yuan6Jiabao Zhang7Department of Laboratory Animals, College of Animal Sciences, Jilin UniversityDepartment of Laboratory Animals, College of Animal Sciences, Jilin UniversityDepartment of Laboratory Animals, College of Animal Sciences, Jilin UniversityThe Second Clinical School of Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine-Zhuhai Hospital, Guangzhou University of Chinese MedicineDepartment of Laboratory Animals, College of Animal Sciences, Jilin UniversityDepartment of Laboratory Animals, College of Animal Sciences, Jilin UniversityDepartment of Laboratory Animals, College of Animal Sciences, Jilin UniversityDepartment of Laboratory Animals, College of Animal Sciences, Jilin UniversityAbstract Eupafolin is a flavonoid that can be extracted from common sage. Previous studies have reported that Eupafolin has antioxidant, anti-inflammatory and anti-tumor properties. However, no studies have investigated the role of Eupafolin in breast cancer. Herein, we investigated the effect of Eupafolin on two human breast cancer cell lines, as well as its potential mechanism of action. Next, the data showed that proliferation, migration and invasion ability of breast cancer cells that were treated with Eupafolin was significantly reduced, while the apoptosis rate was significantly increased. In addition, Eupafolin treatment caused breast cancer cell proliferation to be blocked in the S phase. Moreover, Eupafolin significantly induced autophagy in breast cancer cells, with an increase in the expression of LC3B-II. PI3K/AKT, MAPKs and NF-κB pathways were significantly inhibited by Eupafolin treatment. Additionally, 3-MA (a blocker of autophagosome formation) significantly reduced Eupafolin-induced activation of LC3B-II in breast cancer cells. Furthermore, Eupafolin displayed good in vitro anti-angiogenic activity. Additionally, anti-breast cancer activity of Eupafolin was found to be partially mediated by Cav-1. Moreover, Eupafolin treatment significantly weakened carcinogenesis of MCF-7 cells in nude mice. Therefore, this data provides novel directions on the use of Eupafolin for treatment of breast cancer.https://doi.org/10.1038/s41598-021-00945-9 |
| spellingShingle | Jiahui Wei Yu Ding Xinmiao Liu Qing Liu Yiran Lu Song He Bao Yuan Jiabao Zhang Eupafolin induces apoptosis and autophagy of breast cancer cells through PI3K/AKT, MAPKs and NF-κB signaling pathways Scientific Reports |
| title | Eupafolin induces apoptosis and autophagy of breast cancer cells through PI3K/AKT, MAPKs and NF-κB signaling pathways |
| title_full | Eupafolin induces apoptosis and autophagy of breast cancer cells through PI3K/AKT, MAPKs and NF-κB signaling pathways |
| title_fullStr | Eupafolin induces apoptosis and autophagy of breast cancer cells through PI3K/AKT, MAPKs and NF-κB signaling pathways |
| title_full_unstemmed | Eupafolin induces apoptosis and autophagy of breast cancer cells through PI3K/AKT, MAPKs and NF-κB signaling pathways |
| title_short | Eupafolin induces apoptosis and autophagy of breast cancer cells through PI3K/AKT, MAPKs and NF-κB signaling pathways |
| title_sort | eupafolin induces apoptosis and autophagy of breast cancer cells through pi3k akt mapks and nf κb signaling pathways |
| url | https://doi.org/10.1038/s41598-021-00945-9 |
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