Evaluation of Age-Dependent Changes in the Coloration of Male Killifish Nothobranchius Guentheri Using New Photoprocessing Methods
Fish as model objects have found wide applications in biology and fundamental medicine and allow studies of behavioral and physiological responses to various environmental factors. Representatives of the genus <i>Nothobranchius</i> are one of the most convenient objects for such studies....
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| Format: | Article |
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MDPI AG
2022-01-01
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| author | Dmitry L. Nikiforov-Nikishin Nikita I. Kochetkov Ekaterina V. Mikodina Alexei L. Nikiforov-Nikishin Yuri G. Simakov Natalya A. Golovacheva Alexander V. Gorbunov Sergei N. Chebotarev Evgeniya Yu. Kirichenko Igor Yu. Zabiyaka Ivan S. Pastukhov Anzhelika B. Bren |
| author_facet | Dmitry L. Nikiforov-Nikishin Nikita I. Kochetkov Ekaterina V. Mikodina Alexei L. Nikiforov-Nikishin Yuri G. Simakov Natalya A. Golovacheva Alexander V. Gorbunov Sergei N. Chebotarev Evgeniya Yu. Kirichenko Igor Yu. Zabiyaka Ivan S. Pastukhov Anzhelika B. Bren |
| author_sort | Dmitry L. Nikiforov-Nikishin |
| collection | DOAJ |
| description | Fish as model objects have found wide applications in biology and fundamental medicine and allow studies of behavioral and physiological responses to various environmental factors. Representatives of the genus <i>Nothobranchius</i> are one of the most convenient objects for such studies. Male fish belonging to the family <i>Nothobranchiidae</i> are characterized by extremely diverse coloration, which constantly changes, depending on the age of the fish, environmental factors, and social hierarchical status. These fish species are characterized by a short life cycle, which allows changes in coloration, an indicator of the ontogenesis stage, to be estimated. Existing methods of fish color assessments do not allow the intensity of coloration of particular body zones to be clearly differentiated. In the present study, we suggest a method of two-factor assessment of specific fish body zones using modified methods of photofixation and image processing software. We describe the protocol of the method and the results of its application to different-aged groups of male <i>Nothobranchius guentheri</i>. The coloration of selected areas (i.e., red spot on the gill cover (RSGC), black border on the caudal fin (BBCF), and white border on the dorsal fin (WBDF)) differed significantly according to the size and age of the fish (<i>p</i> < 0.05). The data obtained suggest that <i>N. guentheri</i> can be a model for studying aging by the intensity of body coloration in males. |
| first_indexed | 2024-03-09T22:35:16Z |
| format | Article |
| id | doaj.art-dfaf4a9c31e74224a22c6088b3c496b1 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T22:35:16Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-dfaf4a9c31e74224a22c6088b3c496b12023-11-23T18:49:39ZengMDPI AGBiology2079-77372022-01-0111220510.3390/biology11020205Evaluation of Age-Dependent Changes in the Coloration of Male Killifish Nothobranchius Guentheri Using New Photoprocessing MethodsDmitry L. Nikiforov-Nikishin0Nikita I. Kochetkov1Ekaterina V. Mikodina2Alexei L. Nikiforov-Nikishin3Yuri G. Simakov4Natalya A. Golovacheva5Alexander V. Gorbunov6Sergei N. Chebotarev7Evgeniya Yu. Kirichenko8Igor Yu. Zabiyaka9Ivan S. Pastukhov10Anzhelika B. Bren11Department of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaDepartment of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaRussian Federal Research Institute of Fisheries and Oceanography (VNIRO), Verkhnyaya Krasnoselskaya St., 17, 107140 Moscow, RussiaDepartment of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaDepartment of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaDepartment of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaDepartment of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaManagement Department, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaFaculty “Bioengineering and Veterinary Medicine”, Don State Technical University, Russian Federation, 1 Gagarin Sq., 344000 Rostov-on-Don, RussiaDepartment of Physical and Applied Material Science, Don State Technical University, Russia Federation, 1 Gagarin Sq., 344000 Rostov-on-Don, RussiaDepartment of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaAcademy of Biology and Biotechnology, Southern Federal University, 344090 Rostov-on-Don, RussiaFish as model objects have found wide applications in biology and fundamental medicine and allow studies of behavioral and physiological responses to various environmental factors. Representatives of the genus <i>Nothobranchius</i> are one of the most convenient objects for such studies. Male fish belonging to the family <i>Nothobranchiidae</i> are characterized by extremely diverse coloration, which constantly changes, depending on the age of the fish, environmental factors, and social hierarchical status. These fish species are characterized by a short life cycle, which allows changes in coloration, an indicator of the ontogenesis stage, to be estimated. Existing methods of fish color assessments do not allow the intensity of coloration of particular body zones to be clearly differentiated. In the present study, we suggest a method of two-factor assessment of specific fish body zones using modified methods of photofixation and image processing software. We describe the protocol of the method and the results of its application to different-aged groups of male <i>Nothobranchius guentheri</i>. The coloration of selected areas (i.e., red spot on the gill cover (RSGC), black border on the caudal fin (BBCF), and white border on the dorsal fin (WBDF)) differed significantly according to the size and age of the fish (<i>p</i> < 0.05). The data obtained suggest that <i>N. guentheri</i> can be a model for studying aging by the intensity of body coloration in males.https://www.mdpi.com/2079-7737/11/2/205coloration<i>Nothobranchius guentheri</i>age-dependent changeslive fish photographyvisualization of morphological characteristics |
| spellingShingle | Dmitry L. Nikiforov-Nikishin Nikita I. Kochetkov Ekaterina V. Mikodina Alexei L. Nikiforov-Nikishin Yuri G. Simakov Natalya A. Golovacheva Alexander V. Gorbunov Sergei N. Chebotarev Evgeniya Yu. Kirichenko Igor Yu. Zabiyaka Ivan S. Pastukhov Anzhelika B. Bren Evaluation of Age-Dependent Changes in the Coloration of Male Killifish Nothobranchius Guentheri Using New Photoprocessing Methods Biology coloration <i>Nothobranchius guentheri</i> age-dependent changes live fish photography visualization of morphological characteristics |
| title | Evaluation of Age-Dependent Changes in the Coloration of Male Killifish Nothobranchius Guentheri Using New Photoprocessing Methods |
| title_full | Evaluation of Age-Dependent Changes in the Coloration of Male Killifish Nothobranchius Guentheri Using New Photoprocessing Methods |
| title_fullStr | Evaluation of Age-Dependent Changes in the Coloration of Male Killifish Nothobranchius Guentheri Using New Photoprocessing Methods |
| title_full_unstemmed | Evaluation of Age-Dependent Changes in the Coloration of Male Killifish Nothobranchius Guentheri Using New Photoprocessing Methods |
| title_short | Evaluation of Age-Dependent Changes in the Coloration of Male Killifish Nothobranchius Guentheri Using New Photoprocessing Methods |
| title_sort | evaluation of age dependent changes in the coloration of male killifish nothobranchius guentheri using new photoprocessing methods |
| topic | coloration <i>Nothobranchius guentheri</i> age-dependent changes live fish photography visualization of morphological characteristics |
| url | https://www.mdpi.com/2079-7737/11/2/205 |
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