Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins
Posttranslational modifications (PTMs) dramatically expand the functional diversity of the proteome. The precise addition and removal of PTMs appears to modulate protein structure and function and control key regulatory processes in living systems. Deciphering how particular PTMs affect protein acti...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/14/4389 |
| _version_ | 1827618827518607360 |
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| author | Omer Harel Muhammad Jbara |
| author_facet | Omer Harel Muhammad Jbara |
| author_sort | Omer Harel |
| collection | DOAJ |
| description | Posttranslational modifications (PTMs) dramatically expand the functional diversity of the proteome. The precise addition and removal of PTMs appears to modulate protein structure and function and control key regulatory processes in living systems. Deciphering how particular PTMs affect protein activity is a current frontier in biology and medicine. The large number of PTMs which can appear in several distinct positions, states, and combinations makes preparing such complex analogs using conventional biological and chemical tools challenging. Strategies to access homogeneous and precisely modified proteins with desired PTMs at selected sites and in feasible quantities are critical to interpreting their molecular code. Here, we summarize recent advances in posttranslational chemical mutagenesis and late-stage functionalization chemistry to transfer novel PTM mimicry into recombinant proteins with emphasis on novel transformations. |
| first_indexed | 2024-03-09T10:14:50Z |
| format | Article |
| id | doaj.art-dfb48e25bd324eb28d668ec29567dbc6 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:14:50Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dfb48e25bd324eb28d668ec29567dbc62023-12-01T22:29:29ZengMDPI AGMolecules1420-30492022-07-012714438910.3390/molecules27144389Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant ProteinsOmer Harel0Muhammad Jbara1School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, IsraelSchool of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, IsraelPosttranslational modifications (PTMs) dramatically expand the functional diversity of the proteome. The precise addition and removal of PTMs appears to modulate protein structure and function and control key regulatory processes in living systems. Deciphering how particular PTMs affect protein activity is a current frontier in biology and medicine. The large number of PTMs which can appear in several distinct positions, states, and combinations makes preparing such complex analogs using conventional biological and chemical tools challenging. Strategies to access homogeneous and precisely modified proteins with desired PTMs at selected sites and in feasible quantities are critical to interpreting their molecular code. Here, we summarize recent advances in posttranslational chemical mutagenesis and late-stage functionalization chemistry to transfer novel PTM mimicry into recombinant proteins with emphasis on novel transformations.https://www.mdpi.com/1420-3049/27/14/4389posttranslational modificationsproteinsalkylationdehydroalaninecysteinebioconjugation |
| spellingShingle | Omer Harel Muhammad Jbara Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins Molecules posttranslational modifications proteins alkylation dehydroalanine cysteine bioconjugation |
| title | Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins |
| title_full | Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins |
| title_fullStr | Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins |
| title_full_unstemmed | Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins |
| title_short | Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins |
| title_sort | posttranslational chemical mutagenesis methods to insert posttranslational modifications into recombinant proteins |
| topic | posttranslational modifications proteins alkylation dehydroalanine cysteine bioconjugation |
| url | https://www.mdpi.com/1420-3049/27/14/4389 |
| work_keys_str_mv | AT omerharel posttranslationalchemicalmutagenesismethodstoinsertposttranslationalmodificationsintorecombinantproteins AT muhammadjbara posttranslationalchemicalmutagenesismethodstoinsertposttranslationalmodificationsintorecombinantproteins |