<i>De Novo</i> Transcriptome Assembly, Functional Annotation and SSR Marker Discovery of Qinling Takin (<i>Budorcas taxicolor bedfordi</i>)

The takin (<i>Budorcas taxicolor</i>) is an endemic ruminant species belonging to the bovine family. The International Union for Conservation of Nature (IUCN) has listed it as an endangered and vulnerable species. However, little is known about its molecular characterization since it lac...

Full description

Bibliographic Details
Main Authors: Ju Qiu, Rui Guo, Yidan Li, Yuyao Zhang, Kangsheng Jia, Yinghu Lei, Linsen Zan, Anning Li
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Animals
Subjects:
Online Access:https://www.mdpi.com/2076-2615/11/8/2366
Description
Summary:The takin (<i>Budorcas taxicolor</i>) is an endemic ruminant species belonging to the bovine family. The International Union for Conservation of Nature (IUCN) has listed it as an endangered and vulnerable species. However, little is known about its molecular characterization since it lacks a reference genome. This study used RNA sequencing followed by <i>de novo</i> assembly, annotation and simple sequence repeats (SSRs) prediction to assess the transcriptome of Qinling takin (<i>Budorcas taxicolor bedfordi</i>) muscles. In total, 21,648 unigenes with an N50 and mean length of 1388 bp and 817 bp, respectively, were successfully detected and annotated against the public databases (NR, GO, KEGG, and EggNOG). Furthermore, 6222 SSRs were identified using the MIcroSAtellite (MISA) identification tool software. Taken together, these findings will provide valuable information for genetic, genomic, and evolutionary studies on takin.
ISSN:2076-2615