Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran
Steinernema feltiae isolate IRAZ13 was recovered from one soil sample collected from the Arasbaran forests, Iran. The new isolate can be distinguished from other isolates by morphologic and morphometric data, and DNA sequences. The first generation male is characterized by spicule length 79 ± 5 (73–...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Journal of Asia-Pacific Biodiversity |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2287884X15000333 |
| _version_ | 1818657380168105984 |
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| author | Mostafa Nikdel Gholamreza Niknam |
| author_facet | Mostafa Nikdel Gholamreza Niknam |
| author_sort | Mostafa Nikdel |
| collection | DOAJ |
| description | Steinernema feltiae isolate IRAZ13 was recovered from one soil sample collected from the Arasbaran forests, Iran. The new isolate can be distinguished from other isolates by morphologic and morphometric data, and DNA sequences. The first generation male is characterized by spicule length 79 ± 5 (73–87) μm, SW ratio [(spicule length/anal body diameter)*100] 160 ± 13 (137–177), GS ratio GS [(gubernaculum/spicule length)*100] 79 ± 8 (62–87), genital papillae arrangement and the second generation male by a mucron 10 ± 1 (9–11) μm long. For the infective juvenile, the body is 883 ± 82 (754–975) μm long, lateral fields with eight equally developed ridges, head smooth, slightly offset, large gonad primordium cells, a long hyaline tail portion (equal to half of tail length), and pore-like phasmid at 41% of the tail length to the semicircle anus are the differentiation factors. Additionally, molecular studies obtained from the 28S-D2/D3 region and ITS1 rDNA sequence analyses and phylogenetic reconstruction further support this nematode as a different isolate. |
| first_indexed | 2024-12-17T03:40:33Z |
| format | Article |
| id | doaj.art-dfc0be89746a413ab7e0cfc47903d189 |
| institution | Directory Open Access Journal |
| issn | 2287-884X |
| language | English |
| last_indexed | 2024-12-17T03:40:33Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Asia-Pacific Biodiversity |
| spelling | doaj.art-dfc0be89746a413ab7e0cfc47903d1892022-12-21T22:05:01ZengElsevierJournal of Asia-Pacific Biodiversity2287-884X2015-06-018214415110.1016/j.japb.2015.04.008Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, IranMostafa Nikdel0Gholamreza Niknam1Forests and Rangelands Research Department, East Azarbaijan Agricultural and Natural Resources Research Center, AREEO, Tabriz, IranDepartment of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, IranSteinernema feltiae isolate IRAZ13 was recovered from one soil sample collected from the Arasbaran forests, Iran. The new isolate can be distinguished from other isolates by morphologic and morphometric data, and DNA sequences. The first generation male is characterized by spicule length 79 ± 5 (73–87) μm, SW ratio [(spicule length/anal body diameter)*100] 160 ± 13 (137–177), GS ratio GS [(gubernaculum/spicule length)*100] 79 ± 8 (62–87), genital papillae arrangement and the second generation male by a mucron 10 ± 1 (9–11) μm long. For the infective juvenile, the body is 883 ± 82 (754–975) μm long, lateral fields with eight equally developed ridges, head smooth, slightly offset, large gonad primordium cells, a long hyaline tail portion (equal to half of tail length), and pore-like phasmid at 41% of the tail length to the semicircle anus are the differentiation factors. Additionally, molecular studies obtained from the 28S-D2/D3 region and ITS1 rDNA sequence analyses and phylogenetic reconstruction further support this nematode as a different isolate.http://www.sciencedirect.com/science/article/pii/S2287884X15000333Arasbaran forestsentomopathogenic nematodemorphologymorphometricsSteinernema feltiae |
| spellingShingle | Mostafa Nikdel Gholamreza Niknam Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran Journal of Asia-Pacific Biodiversity Arasbaran forests entomopathogenic nematode morphology morphometrics Steinernema feltiae |
| title | Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran |
| title_full | Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran |
| title_fullStr | Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran |
| title_full_unstemmed | Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran |
| title_short | Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran |
| title_sort | morphological and molecular characterization of a new isolate of entomopathogenic nematode steinernema feltiae filipjev rhabditida steinernematidae from the arasbaran forests iran |
| topic | Arasbaran forests entomopathogenic nematode morphology morphometrics Steinernema feltiae |
| url | http://www.sciencedirect.com/science/article/pii/S2287884X15000333 |
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