Using metabarcoding and droplet digital PCR to investigate drivers of historical shifts in cyanobacteria from six contrasting lakes
Abstract The frequency and intensity of cyanobacterial blooms is increasing worldwide. Multiple factors are implicated, most of which are anthropogenic. New Zealand provides a useful location to study the impacts of human settlement on lake ecosystems. The first humans (Polynesians) arrived about 75...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14216-8 |
| _version_ | 1828190059210211328 |
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| author | Maïlys Picard Xavier Pochon Javier Atalah John K. Pearman Andrew Rees Jamie D. Howarth Christopher M. Moy Marcus J. Vandergoes Ian Hawes Samiullah Khan Susanna A. Wood |
| author_facet | Maïlys Picard Xavier Pochon Javier Atalah John K. Pearman Andrew Rees Jamie D. Howarth Christopher M. Moy Marcus J. Vandergoes Ian Hawes Samiullah Khan Susanna A. Wood |
| author_sort | Maïlys Picard |
| collection | DOAJ |
| description | Abstract The frequency and intensity of cyanobacterial blooms is increasing worldwide. Multiple factors are implicated, most of which are anthropogenic. New Zealand provides a useful location to study the impacts of human settlement on lake ecosystems. The first humans (Polynesians) arrived about 750 years ago. Following their settlement, there were marked landscape modifications which intensified after European settlement about 150 years ago. The aims of this study were to reconstruct cyanobacterial communities in six lakes over the last 1000 years and explore key drivers of change. Cyanobacterial environmental DNA was extracted from sediment cores and analysed using metabarcoding and droplet digital PCR. Cyanobacteria, including potentially toxic or bloom forming species, were already present in these lakes prior to human arrival, however their overall abundance was low. Total cyanobacteria abundance and richness increased in all lakes after European settlement but was very pronounced in four lakes, where bloom-forming taxa became dominant. These shifts occurred concomitant with land-use change. The catchment of one deteriorated lake is only moderately modified, thus the introduction of non-native fish is posited as the key factor driving this change. The paleolimnological approach used in this study has enabled new insights into timing and potential causes of changes in cyanobacterial communities. |
| first_indexed | 2024-04-12T08:14:58Z |
| format | Article |
| id | doaj.art-dfc0bedddce64692888cf3fd0f0e726d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T08:14:58Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-dfc0bedddce64692888cf3fd0f0e726d2022-12-22T03:40:49ZengNature PortfolioScientific Reports2045-23222022-07-0112111710.1038/s41598-022-14216-8Using metabarcoding and droplet digital PCR to investigate drivers of historical shifts in cyanobacteria from six contrasting lakesMaïlys Picard0Xavier Pochon1Javier Atalah2John K. Pearman3Andrew Rees4Jamie D. Howarth5Christopher M. Moy6Marcus J. Vandergoes7Ian Hawes8Samiullah Khan9Susanna A. Wood10Coastal and Freshwater Group, Cawthron InstituteCoastal and Freshwater Group, Cawthron InstituteCoastal and Freshwater Group, Cawthron InstituteCoastal and Freshwater Group, Cawthron InstituteSchool of Geography, Environment and Earth Sciences, Victoria University of WellingtonSchool of Geography, Environment and Earth Sciences, Victoria University of WellingtonDepartment of Zoology, University of OtagoGNS ScienceDepartment of Biological Sciences, School of Biological Sciences, University of WaikatoDepartment of Zoology, University of OtagoCoastal and Freshwater Group, Cawthron InstituteAbstract The frequency and intensity of cyanobacterial blooms is increasing worldwide. Multiple factors are implicated, most of which are anthropogenic. New Zealand provides a useful location to study the impacts of human settlement on lake ecosystems. The first humans (Polynesians) arrived about 750 years ago. Following their settlement, there were marked landscape modifications which intensified after European settlement about 150 years ago. The aims of this study were to reconstruct cyanobacterial communities in six lakes over the last 1000 years and explore key drivers of change. Cyanobacterial environmental DNA was extracted from sediment cores and analysed using metabarcoding and droplet digital PCR. Cyanobacteria, including potentially toxic or bloom forming species, were already present in these lakes prior to human arrival, however their overall abundance was low. Total cyanobacteria abundance and richness increased in all lakes after European settlement but was very pronounced in four lakes, where bloom-forming taxa became dominant. These shifts occurred concomitant with land-use change. The catchment of one deteriorated lake is only moderately modified, thus the introduction of non-native fish is posited as the key factor driving this change. The paleolimnological approach used in this study has enabled new insights into timing and potential causes of changes in cyanobacterial communities.https://doi.org/10.1038/s41598-022-14216-8 |
| spellingShingle | Maïlys Picard Xavier Pochon Javier Atalah John K. Pearman Andrew Rees Jamie D. Howarth Christopher M. Moy Marcus J. Vandergoes Ian Hawes Samiullah Khan Susanna A. Wood Using metabarcoding and droplet digital PCR to investigate drivers of historical shifts in cyanobacteria from six contrasting lakes Scientific Reports |
| title | Using metabarcoding and droplet digital PCR to investigate drivers of historical shifts in cyanobacteria from six contrasting lakes |
| title_full | Using metabarcoding and droplet digital PCR to investigate drivers of historical shifts in cyanobacteria from six contrasting lakes |
| title_fullStr | Using metabarcoding and droplet digital PCR to investigate drivers of historical shifts in cyanobacteria from six contrasting lakes |
| title_full_unstemmed | Using metabarcoding and droplet digital PCR to investigate drivers of historical shifts in cyanobacteria from six contrasting lakes |
| title_short | Using metabarcoding and droplet digital PCR to investigate drivers of historical shifts in cyanobacteria from six contrasting lakes |
| title_sort | using metabarcoding and droplet digital pcr to investigate drivers of historical shifts in cyanobacteria from six contrasting lakes |
| url | https://doi.org/10.1038/s41598-022-14216-8 |
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