Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process
Ecopiling is a method for biodegradation of hydrocarbons in soils. It derives from Biopiles, but phytoremediation is added to biostimulation with nitrogen fertilization and bioaugmentation with local bacteria. We have constructed seven Ecopiles with soil heavily polluted with hydrocarbons in Carlow...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158130/full |
| _version_ | 1797843274280468480 |
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| author | Ruben Martínez-Cuesta Robert Conlon Mutian Wang Esther Blanco-Romero David Durán Miguel Redondo-Nieto David Dowling Daniel Garrido-Sanz Marta Martin Kieran Germaine Rafael Rivilla |
| author_facet | Ruben Martínez-Cuesta Robert Conlon Mutian Wang Esther Blanco-Romero David Durán Miguel Redondo-Nieto David Dowling Daniel Garrido-Sanz Marta Martin Kieran Germaine Rafael Rivilla |
| author_sort | Ruben Martínez-Cuesta |
| collection | DOAJ |
| description | Ecopiling is a method for biodegradation of hydrocarbons in soils. It derives from Biopiles, but phytoremediation is added to biostimulation with nitrogen fertilization and bioaugmentation with local bacteria. We have constructed seven Ecopiles with soil heavily polluted with hydrocarbons in Carlow (Ireland). The aim of the study was to analyze changes in the microbial community during ecopiling. In the course of 18 months of remediation, total petroleum hydrocarbons values decreased in 99 and 88% on average for aliphatics and aromatics, respectively, indicating a successful biodegradation. Community analysis showed that bacterial alfa diversity (Shannon Index), increased with the degradation of hydrocarbons, starting at an average value of 7.59 and ending at an average value of 9.38. Beta-diversity analysis, was performed using Bray-Curtis distances and PCoA ordination, where the two first principal components (PCs) explain the 17 and 14% of the observed variance, respectively. The results show that samples tend to cluster by sampling time instead of by Ecopile. This pattern is supported by the hierarchical clustering analysis, where most samples from the same timepoint clustered together. We used DSeq2 to determine the differential abundance of bacterial populations in Ecopiles at the beginning and the end of the treatment. While TPHs degraders are more abundant at the start of the experiment, these populations are substituted by bacterial populations typical of clean soils by the end of the biodegradation process. Similar results are found for the fungal community, indicating that the microbial community follows a succession along the process. This succession starts with a TPH degraders or tolerant enriched community, and finish with a microbial community typical of clean soils. |
| first_indexed | 2024-04-09T17:02:30Z |
| format | Article |
| id | doaj.art-dfc22778e29b462285cb2aa0fa5035cc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T17:02:30Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dfc22778e29b462285cb2aa0fa5035cc2023-04-21T04:41:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11581301158130Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the processRuben Martínez-Cuesta0Robert Conlon1Mutian Wang2Esther Blanco-Romero3David Durán4Miguel Redondo-Nieto5David Dowling6Daniel Garrido-Sanz7Marta Martin8Kieran Germaine9Rafael Rivilla10Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainEnviroCore, Dargan Research Centre, South East Technological University, Carlow, IrelandEnviroCore, Dargan Research Centre, South East Technological University, Carlow, IrelandDepartamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainDepartamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainDepartamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainEnviroCore, Dargan Research Centre, South East Technological University, Carlow, IrelandDepartamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainDepartamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainEnviroCore, Dargan Research Centre, South East Technological University, Carlow, IrelandDepartamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainEcopiling is a method for biodegradation of hydrocarbons in soils. It derives from Biopiles, but phytoremediation is added to biostimulation with nitrogen fertilization and bioaugmentation with local bacteria. We have constructed seven Ecopiles with soil heavily polluted with hydrocarbons in Carlow (Ireland). The aim of the study was to analyze changes in the microbial community during ecopiling. In the course of 18 months of remediation, total petroleum hydrocarbons values decreased in 99 and 88% on average for aliphatics and aromatics, respectively, indicating a successful biodegradation. Community analysis showed that bacterial alfa diversity (Shannon Index), increased with the degradation of hydrocarbons, starting at an average value of 7.59 and ending at an average value of 9.38. Beta-diversity analysis, was performed using Bray-Curtis distances and PCoA ordination, where the two first principal components (PCs) explain the 17 and 14% of the observed variance, respectively. The results show that samples tend to cluster by sampling time instead of by Ecopile. This pattern is supported by the hierarchical clustering analysis, where most samples from the same timepoint clustered together. We used DSeq2 to determine the differential abundance of bacterial populations in Ecopiles at the beginning and the end of the treatment. While TPHs degraders are more abundant at the start of the experiment, these populations are substituted by bacterial populations typical of clean soils by the end of the biodegradation process. Similar results are found for the fungal community, indicating that the microbial community follows a succession along the process. This succession starts with a TPH degraders or tolerant enriched community, and finish with a microbial community typical of clean soils.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158130/fullEcopilehydrocarbonbioremediationmicrobial successionmicrobiota |
| spellingShingle | Ruben Martínez-Cuesta Robert Conlon Mutian Wang Esther Blanco-Romero David Durán Miguel Redondo-Nieto David Dowling Daniel Garrido-Sanz Marta Martin Kieran Germaine Rafael Rivilla Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process Frontiers in Microbiology Ecopile hydrocarbon bioremediation microbial succession microbiota |
| title | Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process |
| title_full | Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process |
| title_fullStr | Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process |
| title_full_unstemmed | Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process |
| title_short | Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process |
| title_sort | field scale biodegradation of total petroleum hydrocarbons and soil restoration by ecopiles microbiological analysis of the process |
| topic | Ecopile hydrocarbon bioremediation microbial succession microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158130/full |
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