Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation
AbstractFabrication of haemostatic materials with excellent antimicrobial, biocompatible and biodegradable properties remains as a major challenge in the field of medicine. Haemostatic agents play vital role in protecting patients and military individuals during emergency situations. Natural polymer...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2023.2293784 |
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| author | Jayavardhini Bhoopathy Weslen Vedakumari Sathyaraj Beryl Vedha Yesudhason Selvarajan Rajendran Sankari Dharmalingam Jayashri Seetharaman Ranjitha Muthu Ramachandran Murugesan Subramanian Raghunandhakumar Suresh Kumar Anandasadagopan |
| author_facet | Jayavardhini Bhoopathy Weslen Vedakumari Sathyaraj Beryl Vedha Yesudhason Selvarajan Rajendran Sankari Dharmalingam Jayashri Seetharaman Ranjitha Muthu Ramachandran Murugesan Subramanian Raghunandhakumar Suresh Kumar Anandasadagopan |
| author_sort | Jayavardhini Bhoopathy |
| collection | DOAJ |
| description | AbstractFabrication of haemostatic materials with excellent antimicrobial, biocompatible and biodegradable properties remains as a major challenge in the field of medicine. Haemostatic agents play vital role in protecting patients and military individuals during emergency situations. Natural polymers serve as promising materials for fabricating haemostatic compounds due to their efficacy in promoting hemostasis and wound healing. In the present work, sodium alginate/aloe vera/sericin (SA/AV/S) scaffold has been fabricated using a simple cost-effective casting method. The prepared SA/AV/S scaffolds were characterised for their physicochemical properties such as scanning electron microscope, UV–visible spectroscopy and Fourier transform infra-red spectroscopy. SA/AV/S scaffold showed good mechanical strength, swelling behaviour and antibacterial activity. In vitro experiments using erythrocytes proved the hemocompatible and biocompatible features of SA/AV/S scaffold. In vitro blood clotting assay performed using human blood demonstrated the haemostatic and blood absorption properties of SA/AV/S scaffold. Scratch wound assay was performed to study the wound healing efficacy of prepared scaffolds. Chick embryo chorioallantoic membrane assay carried out using fertilised embryos proved the angiogenic property of SA/AV/S scaffold. Thus, SA/AV/S scaffold could serve as a potential haemostatic healthcare product due to its outstanding haemostatic, antimicrobial, hemocompatible, biocompatible and angiogenic properties. |
| first_indexed | 2024-03-08T22:01:33Z |
| format | Article |
| id | doaj.art-dfc37467077942b9a13c8447152113c8 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-08T22:01:33Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-dfc37467077942b9a13c8447152113c82023-12-19T13:11:01ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2024-12-01521354510.1080/21691401.2023.2293784Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluationJayavardhini Bhoopathy0Weslen Vedakumari Sathyaraj1Beryl Vedha Yesudhason2Selvarajan Rajendran3Sankari Dharmalingam4Jayashri Seetharaman5Ranjitha Muthu6Ramachandran Murugesan7Subramanian Raghunandhakumar8Suresh Kumar Anandasadagopan9Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, IndiaFaculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, IndiaRegeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai 600119, IndiaCentre for Nano Science and Technology, Alagappa College of Technology Campus, Anna University, Chennai 600025, Tamil Nadu, IndiaDepartment of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, IndiaDepartment of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, IndiaDepartment of Research, Karpaga Vinayaga Institute of Medical Science and Research Centre, GST Road, Chinna Kolambakkam, Palayanoor (PO), Tamil Nadu 603308, Tamil NaduDepartment of Research, Karpaga Vinayaga Institute of Medical Science and Research Centre, GST Road, Chinna Kolambakkam, Palayanoor (PO), Tamil Nadu 603308, Tamil NaduDepartment of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, Tamil Nadu, IndiaBiochemistry and Biotechnology Laboratory, CSIR-Central Leather Research Institute Adyar 600020, Chennai, IndiaAbstractFabrication of haemostatic materials with excellent antimicrobial, biocompatible and biodegradable properties remains as a major challenge in the field of medicine. Haemostatic agents play vital role in protecting patients and military individuals during emergency situations. Natural polymers serve as promising materials for fabricating haemostatic compounds due to their efficacy in promoting hemostasis and wound healing. In the present work, sodium alginate/aloe vera/sericin (SA/AV/S) scaffold has been fabricated using a simple cost-effective casting method. The prepared SA/AV/S scaffolds were characterised for their physicochemical properties such as scanning electron microscope, UV–visible spectroscopy and Fourier transform infra-red spectroscopy. SA/AV/S scaffold showed good mechanical strength, swelling behaviour and antibacterial activity. In vitro experiments using erythrocytes proved the hemocompatible and biocompatible features of SA/AV/S scaffold. In vitro blood clotting assay performed using human blood demonstrated the haemostatic and blood absorption properties of SA/AV/S scaffold. Scratch wound assay was performed to study the wound healing efficacy of prepared scaffolds. Chick embryo chorioallantoic membrane assay carried out using fertilised embryos proved the angiogenic property of SA/AV/S scaffold. Thus, SA/AV/S scaffold could serve as a potential haemostatic healthcare product due to its outstanding haemostatic, antimicrobial, hemocompatible, biocompatible and angiogenic properties.https://www.tandfonline.com/doi/10.1080/21691401.2023.2293784Sericinscaffoldhemostasisbiocompatibleangiogenesis |
| spellingShingle | Jayavardhini Bhoopathy Weslen Vedakumari Sathyaraj Beryl Vedha Yesudhason Selvarajan Rajendran Sankari Dharmalingam Jayashri Seetharaman Ranjitha Muthu Ramachandran Murugesan Subramanian Raghunandhakumar Suresh Kumar Anandasadagopan Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation Artificial Cells, Nanomedicine, and Biotechnology Sericin scaffold hemostasis biocompatible angiogenesis |
| title | Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation |
| title_full | Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation |
| title_fullStr | Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation |
| title_full_unstemmed | Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation |
| title_short | Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation |
| title_sort | haemostatic potency of sodium alginate aloe vera sericin composite scaffolds preparation characterisation and evaluation |
| topic | Sericin scaffold hemostasis biocompatible angiogenesis |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2023.2293784 |
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