The association of interleukin-6 (−174 G/C) polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsiveness
Introduction: The plasma levels of the cytokine interleukin-6 (IL-6) have been reported to be associated with risk of chronic kidney disease (CKD) and erythropoietin (EPO) responsiveness. The G/C promoter polymorphism of IL-6 is associated with expression and levels of IL-6, so it may confer increas...
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| Format: | Article |
| Language: | English |
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Society of Diabetic Nephropathy Prevention
2022-04-01
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| Series: | Journal of Nephropathology |
| Subjects: | |
| Online Access: | https://nephropathol.com/PDF/jnp-11-e12914.pdf |
| _version_ | 1797828081427152896 |
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| author | Noha Alnair Abdelrhman Elshazali Widaa Ali |
| author_facet | Noha Alnair Abdelrhman Elshazali Widaa Ali |
| author_sort | Noha Alnair Abdelrhman |
| collection | DOAJ |
| description | Introduction: The plasma levels of the cytokine interleukin-6 (IL-6) have been reported to be associated with risk of chronic kidney disease (CKD) and erythropoietin (EPO) responsiveness. The G/C promoter polymorphism of IL-6 is associated with expression and levels of IL-6, so it may confer increased risk to CKD and modulate EPO responsiveness. Objectives: This study aimed to examine the association of IL-6 G/C polymorphism with risk of CKD and EPO hyporesponsiveness. Patients and Methods: A total of 40 haemodialysis patients on EPO therapy, and 30 age- and sexmatched apparently healthy volunteers as a control group were recruited for this study. Blood samples were collected from each participant and used for complete blood count (CBC) using automated haematology analyser and molecular analysis of IL-6 -174 G/C polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The results showed that IL-6 GG genotype is the most frequent in patients (82.5%) followed by GC genotype (17.5%), while all subjects of the control group were found to have GG genotype only. There was a statistically significant association between the polymorphism and end-stage CKD (P=0.02). Only 10% of the patients were found to achieve the target haemoglobin level. Although all of them had GG genotype, no association was found between the polymorphism and the achievement of target haemoglobin level (P=0.16). Conclusion: IL-6 G/C polymorphism is significantly associated with CKD, but not with EPO responsiveness in haemodialysis patients. |
| first_indexed | 2024-04-09T12:58:40Z |
| format | Article |
| id | doaj.art-dfc3dbea933543e1a67b00b138c8e3d0 |
| institution | Directory Open Access Journal |
| issn | 2251-8363 2251-8819 |
| language | English |
| last_indexed | 2024-04-09T12:58:40Z |
| publishDate | 2022-04-01 |
| publisher | Society of Diabetic Nephropathy Prevention |
| record_format | Article |
| series | Journal of Nephropathology |
| spelling | doaj.art-dfc3dbea933543e1a67b00b138c8e3d02023-05-13T11:04:09ZengSociety of Diabetic Nephropathy PreventionJournal of Nephropathology2251-83632251-88192022-04-01112e12914e1291410.34172/jnp.2022.12914jnp-12914The association of interleukin-6 (−174 G/C) polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsivenessNoha Alnair Abdelrhman0Elshazali Widaa Ali1Faculty of Medical Laboratory Sciences, El Neelain University, Khartoum, SudanCollege of Applied Medical Sciences, University of Bisha, Bisha, Saudi ArabiaIntroduction: The plasma levels of the cytokine interleukin-6 (IL-6) have been reported to be associated with risk of chronic kidney disease (CKD) and erythropoietin (EPO) responsiveness. The G/C promoter polymorphism of IL-6 is associated with expression and levels of IL-6, so it may confer increased risk to CKD and modulate EPO responsiveness. Objectives: This study aimed to examine the association of IL-6 G/C polymorphism with risk of CKD and EPO hyporesponsiveness. Patients and Methods: A total of 40 haemodialysis patients on EPO therapy, and 30 age- and sexmatched apparently healthy volunteers as a control group were recruited for this study. Blood samples were collected from each participant and used for complete blood count (CBC) using automated haematology analyser and molecular analysis of IL-6 -174 G/C polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The results showed that IL-6 GG genotype is the most frequent in patients (82.5%) followed by GC genotype (17.5%), while all subjects of the control group were found to have GG genotype only. There was a statistically significant association between the polymorphism and end-stage CKD (P=0.02). Only 10% of the patients were found to achieve the target haemoglobin level. Although all of them had GG genotype, no association was found between the polymorphism and the achievement of target haemoglobin level (P=0.16). Conclusion: IL-6 G/C polymorphism is significantly associated with CKD, but not with EPO responsiveness in haemodialysis patients.https://nephropathol.com/PDF/jnp-11-e12914.pdfchronic kidney diseaseinterleukin-6 -174 g/c polymorphismerythropoietin responsivenesstarget hemoglobin level |
| spellingShingle | Noha Alnair Abdelrhman Elshazali Widaa Ali The association of interleukin-6 (−174 G/C) polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsiveness Journal of Nephropathology chronic kidney disease interleukin-6 -174 g/c polymorphism erythropoietin responsiveness target hemoglobin level |
| title | The association of interleukin-6 (−174 G/C) polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsiveness |
| title_full | The association of interleukin-6 (−174 G/C) polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsiveness |
| title_fullStr | The association of interleukin-6 (−174 G/C) polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsiveness |
| title_full_unstemmed | The association of interleukin-6 (−174 G/C) polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsiveness |
| title_short | The association of interleukin-6 (−174 G/C) polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsiveness |
| title_sort | association of interleukin 6 174 g c polymorphism with risk of chronic kidney disease and erythropoietin hyporesponsiveness |
| topic | chronic kidney disease interleukin-6 -174 g/c polymorphism erythropoietin responsiveness target hemoglobin level |
| url | https://nephropathol.com/PDF/jnp-11-e12914.pdf |
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