Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors
Lignocellulosic hydrolysate (LCH) inhibitors are a large class of bioactive molecules that arise from pretreatment, hydrolysis, and fermentation of plant biomass. These diverse compounds reduce lignocellulosic biofuel yields by inhibiting cellular processes and diverting energy into cellular respons...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-03-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00090/full |
| _version_ | 1819262438029131776 |
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| author | Jeff Scott Piotrowski Yaoping eZhang Trey eSato Irene eOng David eKeating Donna eBates Robert eLandick |
| author_facet | Jeff Scott Piotrowski Yaoping eZhang Trey eSato Irene eOng David eKeating Donna eBates Robert eLandick |
| author_sort | Jeff Scott Piotrowski |
| collection | DOAJ |
| description | Lignocellulosic hydrolysate (LCH) inhibitors are a large class of bioactive molecules that arise from pretreatment, hydrolysis, and fermentation of plant biomass. These diverse compounds reduce lignocellulosic biofuel yields by inhibiting cellular processes and diverting energy into cellular responses. LCH inhibitors present one of the most significant challenges to efficient biofuel production by microbes. Development of new strains that lessen the effects of LCH inhibitors is an economically favorable strategy relative to expensive detoxification methods that also can reduce sugar content in deconstructed biomass. Systems biology analyses and metabolic modeling combined with directed evolution and synthetic biology are successful strategies for biocatalyst development, and methods that leverage state-of-the-art tools are needed to overcome inhibitors more completely. This perspective considers the energetic costs of LCH inhibitors and technologies that can be used to overcome their drain on conversion efficiency. We suggest academic and commercial research groups could benefit by sharing data on LCH inhibitors and implementing translational biofuel research. |
| first_indexed | 2024-12-23T19:57:41Z |
| format | Article |
| id | doaj.art-dfc53d08782347509c7d5b49ed62950e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T19:57:41Z |
| publishDate | 2014-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dfc53d08782347509c7d5b49ed62950e2022-12-21T17:33:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-03-01510.3389/fmicb.2014.0009075595Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitorsJeff Scott Piotrowski0Yaoping eZhang1Trey eSato2Irene eOng3David eKeating4Donna eBates5Robert eLandick6Great Lakes Bioenergy Research Center, Unversity or Wisconsin-MadisonGreat Lakes Bioenergy Research Center, Unversity or Wisconsin-MadisonGreat Lakes Bioenergy Research Center, Unversity or Wisconsin-MadisonGreat Lakes Bioenergy Research Center, Unversity or Wisconsin-MadisonGreat Lakes Bioenergy Research Center, Unversity or Wisconsin-MadisonGreat Lakes Bioenergy Research Center, Unversity or Wisconsin-MadisonGreat Lakes Bioenergy Research Center, Unversity or Wisconsin-MadisonLignocellulosic hydrolysate (LCH) inhibitors are a large class of bioactive molecules that arise from pretreatment, hydrolysis, and fermentation of plant biomass. These diverse compounds reduce lignocellulosic biofuel yields by inhibiting cellular processes and diverting energy into cellular responses. LCH inhibitors present one of the most significant challenges to efficient biofuel production by microbes. Development of new strains that lessen the effects of LCH inhibitors is an economically favorable strategy relative to expensive detoxification methods that also can reduce sugar content in deconstructed biomass. Systems biology analyses and metabolic modeling combined with directed evolution and synthetic biology are successful strategies for biocatalyst development, and methods that leverage state-of-the-art tools are needed to overcome inhibitors more completely. This perspective considers the energetic costs of LCH inhibitors and technologies that can be used to overcome their drain on conversion efficiency. We suggest academic and commercial research groups could benefit by sharing data on LCH inhibitors and implementing translational biofuel research.http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00090/fullSystems Biologymetabolic modelingChemical genomicscellulosic biofuelsLignocellulosic hydrolysate inhibitorsethanologens |
| spellingShingle | Jeff Scott Piotrowski Yaoping eZhang Trey eSato Irene eOng David eKeating Donna eBates Robert eLandick Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors Frontiers in Microbiology Systems Biology metabolic modeling Chemical genomics cellulosic biofuels Lignocellulosic hydrolysate inhibitors ethanologens |
| title | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
| title_full | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
| title_fullStr | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
| title_full_unstemmed | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
| title_short | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
| title_sort | death by a thousand cuts the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
| topic | Systems Biology metabolic modeling Chemical genomics cellulosic biofuels Lignocellulosic hydrolysate inhibitors ethanologens |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00090/full |
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