Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants
Triploid is considered a reproductive barrier and also a bridge in the formation of polyploids. However, few reports are available in Cymbidium. In this study, diploid ‘Xiaofeng’, sexual triploid ‘Yuchan’ and ‘Huanghe’ of Cymbidium were used to evaluate hybridization compatibility of the triploids....
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| Format: | Article |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1029915/full |
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| author | Man-Man Li Qing-Lian Su Jun-Rui Zu Li Xie Qian Wei He-Rong Guo Jianjun Chen Rui-Zhen Zeng Zhi-Sheng Zhang |
| author_facet | Man-Man Li Qing-Lian Su Jun-Rui Zu Li Xie Qian Wei He-Rong Guo Jianjun Chen Rui-Zhen Zeng Zhi-Sheng Zhang |
| author_sort | Man-Man Li |
| collection | DOAJ |
| description | Triploid is considered a reproductive barrier and also a bridge in the formation of polyploids. However, few reports are available in Cymbidium. In this study, diploid ‘Xiaofeng’, sexual triploid ‘Yuchan’ and ‘Huanghe’ of Cymbidium were used to evaluate hybridization compatibility of the triploids. Results showed that the sexual triploids were fertile whether they were used as male or female parents. ‘Yuchan’ produced male gametes of 1x, 1x~2x, 2x, 2x~3x, and 3x at frequencies of 8.89%, 77.78%, 6.67%, 3.33%, and 3.33%, respectively; while ‘Huanghe’ produced 3.33% 1x, 80.00% 1x~2x, 8.89% 2x, 5.56% 2x~3x, and 2.22% 3x male gametes. The cross of ‘Xiaofeng’ with ‘Yuchan’ produced progenies with a wide range of ploidy levels, including one diploid, 34 2×~3× aneuploids, 12 triploids, and one tetraploid, indicating that male gametes produced by sexual triploid were fertile and could be transmitted and fused with egg cells. On the other hand, 10 progenies obtained from the cross of ‘Yuchan’ × ‘Xiaofeng’ were all aneuploids. The cross of ‘Yuchan’ with ‘Huanghe’ produced 40 progenies including three 2×~3× aneuploids, nine 3×~4× aneuploids, 21 tetraploids, six 4×~5× aneuploids, and one pentaploid, suggesting that 2x gametes, instead of the unreduced ones played a more important role in the formation of tetraploids. The survival rates of the hybrids were all above 80.00%, with the tetraploids at 96.67%. Cytological analysis revealed that during meiosis of sexual polyploids, two chromosome sets of the 2n gamete were inclined to enter into the same daughter cell, resulting in the production of 2x gametes. Our results indicate that the triploid cymbidiums are not reproductive barrier but serve as a bridge in the formation of polyploid plants. |
| first_indexed | 2024-04-11T00:35:20Z |
| format | Article |
| id | doaj.art-dfc6fbe7d5ae4290b79e6596f9fb6230 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T00:35:20Z |
| publishDate | 2022-10-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-dfc6fbe7d5ae4290b79e6596f9fb62302023-01-06T20:27:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10299151029915Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plantsMan-Man Li0Qing-Lian Su1Jun-Rui Zu2Li Xie3Qian Wei4He-Rong Guo5Jianjun Chen6Rui-Zhen Zeng7Zhi-Sheng Zhang8Guangdong Province Key Laboratory of Plant Molecular Breeding, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangzhou Flower Research Center, Guangzhou, ChinaGuangdong Province Key Laboratory of Plant Molecular Breeding, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Plant Molecular Breeding, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Plant Molecular Breeding, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Plant Molecular Breeding, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaMid-Florida Research and Education Center, Environmental Horticulture Department, Institute of Food and Agricultural Sciences, University of Florida, Apopka, FL, United StatesGuangdong Province Key Laboratory of Plant Molecular Breeding, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Plant Molecular Breeding, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaTriploid is considered a reproductive barrier and also a bridge in the formation of polyploids. However, few reports are available in Cymbidium. In this study, diploid ‘Xiaofeng’, sexual triploid ‘Yuchan’ and ‘Huanghe’ of Cymbidium were used to evaluate hybridization compatibility of the triploids. Results showed that the sexual triploids were fertile whether they were used as male or female parents. ‘Yuchan’ produced male gametes of 1x, 1x~2x, 2x, 2x~3x, and 3x at frequencies of 8.89%, 77.78%, 6.67%, 3.33%, and 3.33%, respectively; while ‘Huanghe’ produced 3.33% 1x, 80.00% 1x~2x, 8.89% 2x, 5.56% 2x~3x, and 2.22% 3x male gametes. The cross of ‘Xiaofeng’ with ‘Yuchan’ produced progenies with a wide range of ploidy levels, including one diploid, 34 2×~3× aneuploids, 12 triploids, and one tetraploid, indicating that male gametes produced by sexual triploid were fertile and could be transmitted and fused with egg cells. On the other hand, 10 progenies obtained from the cross of ‘Yuchan’ × ‘Xiaofeng’ were all aneuploids. The cross of ‘Yuchan’ with ‘Huanghe’ produced 40 progenies including three 2×~3× aneuploids, nine 3×~4× aneuploids, 21 tetraploids, six 4×~5× aneuploids, and one pentaploid, suggesting that 2x gametes, instead of the unreduced ones played a more important role in the formation of tetraploids. The survival rates of the hybrids were all above 80.00%, with the tetraploids at 96.67%. Cytological analysis revealed that during meiosis of sexual polyploids, two chromosome sets of the 2n gamete were inclined to enter into the same daughter cell, resulting in the production of 2x gametes. Our results indicate that the triploid cymbidiums are not reproductive barrier but serve as a bridge in the formation of polyploid plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.1029915/fullcross compatibilityCymbidiumpolyploidytriploid bridgeunreduced gamete |
| spellingShingle | Man-Man Li Qing-Lian Su Jun-Rui Zu Li Xie Qian Wei He-Rong Guo Jianjun Chen Rui-Zhen Zeng Zhi-Sheng Zhang Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants Frontiers in Plant Science cross compatibility Cymbidium polyploidy triploid bridge unreduced gamete |
| title | Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants |
| title_full | Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants |
| title_fullStr | Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants |
| title_full_unstemmed | Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants |
| title_short | Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants |
| title_sort | triploid cultivars of cymbidium act as a bridge in the formation of polyploid plants |
| topic | cross compatibility Cymbidium polyploidy triploid bridge unreduced gamete |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1029915/full |
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