Impact of Superparamagnetic Iron Oxide Nanoparticles on THP-1 Monocytes and Monocyte-Derived Macrophages
Superparamagnetic iron oxide nanoparticles (SPIONs) are currently under examination for magnetic particle imaging, which represents a radiation free technology for three-dimensional imaging with high sensitivity, resolution and imaging speed. SPIONs are rapidly taken up by monocytes and other phagoc...
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| Format: | Article |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.811116/full |
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| author | Christina Polasky Tim Studt Ann-Kathrin Steuer Ann-Kathrin Steuer Kristin Loyal Kerstin Lüdtke-Buzug Karl-Ludwig Bruchhage Ralph Pries |
| author_facet | Christina Polasky Tim Studt Ann-Kathrin Steuer Ann-Kathrin Steuer Kristin Loyal Kerstin Lüdtke-Buzug Karl-Ludwig Bruchhage Ralph Pries |
| author_sort | Christina Polasky |
| collection | DOAJ |
| description | Superparamagnetic iron oxide nanoparticles (SPIONs) are currently under examination for magnetic particle imaging, which represents a radiation free technology for three-dimensional imaging with high sensitivity, resolution and imaging speed. SPIONs are rapidly taken up by monocytes and other phagocytes which carry them to the site of inflammation. Therefore, the SPION biocompatibility is an essential parameter for a widespread MPI usage. Many improvements are expected from SPION development and its applications for cell visualization, but the impact of MPI optimized dextran coated SPIONs on the cellular characteristics of monocytic cells has been poorly studied up to now. THP-1 monocytes, monocyte-derived macrophages (MDM) as well as peripheral blood monocytes were incubated with MPI-optimized dextran-coated SPIONs of a size between 83.5 and 86 nm. SPION uptake was measured by FITC fluorescence of labeled SPIONs and Prussian blue staining. The activation of monocytes and MDMs was evaluated by CD14, CD11b and CD86 in flow cytometry. The secretion of IL-1β, and IL-10 was analyzed in supernatants. SPIONs were rapidly taken up by monocytes and monocyte-derived macrophages while no decrease in cell viability was observed. Expression patterns of CD11b, CD14, and CD86 were not affected in THP-1 monocytes and MDMs. Monocyte differentiation in macrophages was hindered during SPION uptake. THP-1 monocytes as well as monocyte-derived macrophages showed significantly increased IL-1β and decreased IL-10 secretion by tendency after SPION treatment. Dextran-coated SPIONs showed a low cytotoxicity on monocytes but exert undesirable inflammatory side effects that have to be considered for imaging applications. |
| first_indexed | 2024-12-19T21:23:53Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-19T21:23:53Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-dfc8ed5b09e04dddbd7781c173ab81842022-12-21T20:05:11ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-02-01910.3389/fmolb.2022.811116811116Impact of Superparamagnetic Iron Oxide Nanoparticles on THP-1 Monocytes and Monocyte-Derived MacrophagesChristina Polasky0Tim Studt1Ann-Kathrin Steuer2Ann-Kathrin Steuer3Kristin Loyal4Kerstin Lüdtke-Buzug5Karl-Ludwig Bruchhage6Ralph Pries7Department of Otorhinolaryngology, University Hospital of Schleswig-Holstein, Luebeck, GermanyDepartment of Otorhinolaryngology, University Hospital of Schleswig-Holstein, Luebeck, GermanyInstitute of Medical Engineering, University of Luebeck, Luebeck, GermanyFraunhofer Research Institution for Individualized and Cell-Based Medical Engineering, Luebeck, GermanyDepartment of Otorhinolaryngology, University Hospital of Schleswig-Holstein, Luebeck, GermanyInstitute of Medical Engineering, University of Luebeck, Luebeck, GermanyDepartment of Otorhinolaryngology, University Hospital of Schleswig-Holstein, Luebeck, GermanyDepartment of Otorhinolaryngology, University Hospital of Schleswig-Holstein, Luebeck, GermanySuperparamagnetic iron oxide nanoparticles (SPIONs) are currently under examination for magnetic particle imaging, which represents a radiation free technology for three-dimensional imaging with high sensitivity, resolution and imaging speed. SPIONs are rapidly taken up by monocytes and other phagocytes which carry them to the site of inflammation. Therefore, the SPION biocompatibility is an essential parameter for a widespread MPI usage. Many improvements are expected from SPION development and its applications for cell visualization, but the impact of MPI optimized dextran coated SPIONs on the cellular characteristics of monocytic cells has been poorly studied up to now. THP-1 monocytes, monocyte-derived macrophages (MDM) as well as peripheral blood monocytes were incubated with MPI-optimized dextran-coated SPIONs of a size between 83.5 and 86 nm. SPION uptake was measured by FITC fluorescence of labeled SPIONs and Prussian blue staining. The activation of monocytes and MDMs was evaluated by CD14, CD11b and CD86 in flow cytometry. The secretion of IL-1β, and IL-10 was analyzed in supernatants. SPIONs were rapidly taken up by monocytes and monocyte-derived macrophages while no decrease in cell viability was observed. Expression patterns of CD11b, CD14, and CD86 were not affected in THP-1 monocytes and MDMs. Monocyte differentiation in macrophages was hindered during SPION uptake. THP-1 monocytes as well as monocyte-derived macrophages showed significantly increased IL-1β and decreased IL-10 secretion by tendency after SPION treatment. Dextran-coated SPIONs showed a low cytotoxicity on monocytes but exert undesirable inflammatory side effects that have to be considered for imaging applications.https://www.frontiersin.org/articles/10.3389/fmolb.2022.811116/fullMPImonocytesiron oxide nanoparticlesbiocompatibilitydifferentiation |
| spellingShingle | Christina Polasky Tim Studt Ann-Kathrin Steuer Ann-Kathrin Steuer Kristin Loyal Kerstin Lüdtke-Buzug Karl-Ludwig Bruchhage Ralph Pries Impact of Superparamagnetic Iron Oxide Nanoparticles on THP-1 Monocytes and Monocyte-Derived Macrophages Frontiers in Molecular Biosciences MPI monocytes iron oxide nanoparticles biocompatibility differentiation |
| title | Impact of Superparamagnetic Iron Oxide Nanoparticles on THP-1 Monocytes and Monocyte-Derived Macrophages |
| title_full | Impact of Superparamagnetic Iron Oxide Nanoparticles on THP-1 Monocytes and Monocyte-Derived Macrophages |
| title_fullStr | Impact of Superparamagnetic Iron Oxide Nanoparticles on THP-1 Monocytes and Monocyte-Derived Macrophages |
| title_full_unstemmed | Impact of Superparamagnetic Iron Oxide Nanoparticles on THP-1 Monocytes and Monocyte-Derived Macrophages |
| title_short | Impact of Superparamagnetic Iron Oxide Nanoparticles on THP-1 Monocytes and Monocyte-Derived Macrophages |
| title_sort | impact of superparamagnetic iron oxide nanoparticles on thp 1 monocytes and monocyte derived macrophages |
| topic | MPI monocytes iron oxide nanoparticles biocompatibility differentiation |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.811116/full |
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