A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids
The valuable marine carotenoid, astaxanthin, is used in supplements, medicines and cosmetics. In this study, crustacyanin, an astaxanthin-binding protein, was used to solubilize and concentrate astaxanthin. The recombinant crustacyanin of European lobster spontaneously formed an inclusion body when...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/8/462 |
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| author | Kiyotaka Y. Hara Shuwa Yagi Yoko Hirono-Hara Hiroshi Kikukawa |
| author_facet | Kiyotaka Y. Hara Shuwa Yagi Yoko Hirono-Hara Hiroshi Kikukawa |
| author_sort | Kiyotaka Y. Hara |
| collection | DOAJ |
| description | The valuable marine carotenoid, astaxanthin, is used in supplements, medicines and cosmetics. In this study, crustacyanin, an astaxanthin-binding protein, was used to solubilize and concentrate astaxanthin. The recombinant crustacyanin of European lobster spontaneously formed an inclusion body when it was over-expressed in <i>Escherichia coli</i>. In this study, fusing the NusA-tag to the crustacyanin subunits made it possible to express in a soluble fraction and solubilize astaxanthin in aqueous solution. By cutting off the NusA-tag, the crustacyanin subunits generated the pure insoluble form, and captured and concentrated astaxanthin. Overall, the attaching and releasing NusA-tag method has the potential to supply solubilized carotenoids in aqueous solution and concentrated carotenoids, respectively. |
| first_indexed | 2024-03-10T08:39:32Z |
| format | Article |
| id | doaj.art-dfc9f6fd9f5248b19065a9007cfaf611 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T08:39:32Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-dfc9f6fd9f5248b19065a9007cfaf6112023-11-22T08:26:03ZengMDPI AGMarine Drugs1660-33972021-08-0119846210.3390/md19080462A Method of Solubilizing and Concentrating Astaxanthin and Other CarotenoidsKiyotaka Y. Hara0Shuwa Yagi1Yoko Hirono-Hara2Hiroshi Kikukawa3Laboratory of Environmental Bioengineering, Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, JapanLaboratory of Environmental Bioengineering, Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, JapanDepartment of Environmental and Life Sciences, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, JapanLaboratory of Environmental Bioengineering, Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, JapanThe valuable marine carotenoid, astaxanthin, is used in supplements, medicines and cosmetics. In this study, crustacyanin, an astaxanthin-binding protein, was used to solubilize and concentrate astaxanthin. The recombinant crustacyanin of European lobster spontaneously formed an inclusion body when it was over-expressed in <i>Escherichia coli</i>. In this study, fusing the NusA-tag to the crustacyanin subunits made it possible to express in a soluble fraction and solubilize astaxanthin in aqueous solution. By cutting off the NusA-tag, the crustacyanin subunits generated the pure insoluble form, and captured and concentrated astaxanthin. Overall, the attaching and releasing NusA-tag method has the potential to supply solubilized carotenoids in aqueous solution and concentrated carotenoids, respectively.https://www.mdpi.com/1660-3397/19/8/462astaxanthinmarine carotenoidcrustacyaninEuropean lobsterNusA-tag<i>Escherichia coli</i> |
| spellingShingle | Kiyotaka Y. Hara Shuwa Yagi Yoko Hirono-Hara Hiroshi Kikukawa A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids Marine Drugs astaxanthin marine carotenoid crustacyanin European lobster NusA-tag <i>Escherichia coli</i> |
| title | A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids |
| title_full | A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids |
| title_fullStr | A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids |
| title_full_unstemmed | A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids |
| title_short | A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids |
| title_sort | method of solubilizing and concentrating astaxanthin and other carotenoids |
| topic | astaxanthin marine carotenoid crustacyanin European lobster NusA-tag <i>Escherichia coli</i> |
| url | https://www.mdpi.com/1660-3397/19/8/462 |
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