Synthesis and Structural Characterization of CaO-P<sub>2</sub>O<sub>5</sub>-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer Cells
Copper is one of the most used therapeutic metallic elements in biomedicine, ranging from antibacterial approaches to developing new complexes in cancer therapy. In the present investigation, we developed a novel xCuO∙(100 − x) [CaF<sub>2</sub>∙3P<sub>2</sub>O<sub>5<...
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| Online Access: | https://www.mdpi.com/1996-1944/15/4/1526 |
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| author | Nicoleta Simona Vedeanu Cristian Lujerdean Marius Zăhan Daniel Severus Dezmirean Lucian Barbu-Tudoran Grigore Damian Răzvan Ștefan |
| author_facet | Nicoleta Simona Vedeanu Cristian Lujerdean Marius Zăhan Daniel Severus Dezmirean Lucian Barbu-Tudoran Grigore Damian Răzvan Ștefan |
| author_sort | Nicoleta Simona Vedeanu |
| collection | DOAJ |
| description | Copper is one of the most used therapeutic metallic elements in biomedicine, ranging from antibacterial approaches to developing new complexes in cancer therapy. In the present investigation, we developed a novel xCuO∙(100 − x) [CaF<sub>2</sub>∙3P<sub>2</sub>O<sub>5</sub>∙CaO] glass system with 0 ≤ x ≤ 16 mol% in order to determine the influence of doping on the composition structure of glasses. The samples were characterized by dissolution tests, pH measurements, Fourier-transform infrared spectroscopy (FT-IR), electron paramagnetic resonance (EPR), Scanning Electron Microscopy with energy dispersive spectroscopy (SEM-EDX) and afterward, their antitumor character was assessed. The glasses were mostly soluble in the aqueous medium, their dissolution rate being directly proportional to the increase in pH and the level of doping up to x = 8 mol%. FT-IR spectra of glass samples show the presence of all structural units characteristic to P<sub>2</sub>O<sub>5</sub> in different rates and directly depending on the depolymerization process. SEM-EDX results revealed the presence of an amorphous glass structure composed of P, O, Ca, and Cu elements. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay showed strong cytotoxicity for tumoral cells A375 even in low concentrations for Cu-treatment. In contrast, the copper-free matrix (without Cu) determined a proliferative effect of over 70% viability for all concentrations used. |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T21:31:54Z |
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| spelling | doaj.art-dfcb03433ee5460288d23d4d47042ed02023-11-23T20:54:41ZengMDPI AGMaterials1996-19442022-02-01154152610.3390/ma15041526Synthesis and Structural Characterization of CaO-P<sub>2</sub>O<sub>5</sub>-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer CellsNicoleta Simona Vedeanu0Cristian Lujerdean1Marius Zăhan2Daniel Severus Dezmirean3Lucian Barbu-Tudoran4Grigore Damian5Răzvan Ștefan6Department of Pharmaceutical Physics-Biophysics, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, Pasteur 6, 400349 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Animal Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Animal Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Animal Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, RomaniaElectron Microscopy Center “Prof. C. Craciun”, Faculty of Biology & Geology, “Babes-Bolyai” University, 5-7 Clinicilor St., 400006 Cluj-Napoca, RomaniaFaculty of Physics, Babes-Bolyai University, 400084 Cluj-Napoca, RomaniaFaculty of Animal Science and Biotechnology, University of Animal Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, RomaniaCopper is one of the most used therapeutic metallic elements in biomedicine, ranging from antibacterial approaches to developing new complexes in cancer therapy. In the present investigation, we developed a novel xCuO∙(100 − x) [CaF<sub>2</sub>∙3P<sub>2</sub>O<sub>5</sub>∙CaO] glass system with 0 ≤ x ≤ 16 mol% in order to determine the influence of doping on the composition structure of glasses. The samples were characterized by dissolution tests, pH measurements, Fourier-transform infrared spectroscopy (FT-IR), electron paramagnetic resonance (EPR), Scanning Electron Microscopy with energy dispersive spectroscopy (SEM-EDX) and afterward, their antitumor character was assessed. The glasses were mostly soluble in the aqueous medium, their dissolution rate being directly proportional to the increase in pH and the level of doping up to x = 8 mol%. FT-IR spectra of glass samples show the presence of all structural units characteristic to P<sub>2</sub>O<sub>5</sub> in different rates and directly depending on the depolymerization process. SEM-EDX results revealed the presence of an amorphous glass structure composed of P, O, Ca, and Cu elements. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay showed strong cytotoxicity for tumoral cells A375 even in low concentrations for Cu-treatment. In contrast, the copper-free matrix (without Cu) determined a proliferative effect of over 70% viability for all concentrations used.https://www.mdpi.com/1996-1944/15/4/1526phosphate glassdissolution testscopper ionsFourier transform infrared (FTIR) spectraantitumor activityMTT assay |
| spellingShingle | Nicoleta Simona Vedeanu Cristian Lujerdean Marius Zăhan Daniel Severus Dezmirean Lucian Barbu-Tudoran Grigore Damian Răzvan Ștefan Synthesis and Structural Characterization of CaO-P<sub>2</sub>O<sub>5</sub>-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer Cells Materials phosphate glass dissolution tests copper ions Fourier transform infrared (FTIR) spectra antitumor activity MTT assay |
| title | Synthesis and Structural Characterization of CaO-P<sub>2</sub>O<sub>5</sub>-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer Cells |
| title_full | Synthesis and Structural Characterization of CaO-P<sub>2</sub>O<sub>5</sub>-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer Cells |
| title_fullStr | Synthesis and Structural Characterization of CaO-P<sub>2</sub>O<sub>5</sub>-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer Cells |
| title_full_unstemmed | Synthesis and Structural Characterization of CaO-P<sub>2</sub>O<sub>5</sub>-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer Cells |
| title_short | Synthesis and Structural Characterization of CaO-P<sub>2</sub>O<sub>5</sub>-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer Cells |
| title_sort | synthesis and structural characterization of cao p sub 2 sub o sub 5 sub caf cuo glasses with antitumoral effect on skin cancer cells |
| topic | phosphate glass dissolution tests copper ions Fourier transform infrared (FTIR) spectra antitumor activity MTT assay |
| url | https://www.mdpi.com/1996-1944/15/4/1526 |
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