Beyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant <em>Staphylococcus aureus</em> Genetic Lineages Circulating in Spanish Hospitals
Tetracycline resistance (Tet<sup>R</sup>) has been evidenced as a good phenotypic marker for detection of livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) isolates of the clonal complex CC398. The aim of this study was to characterise a collec...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/3/307 |
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| author | Sara Ceballos Carmen Lozano Carmen Aspiroz Laura Ruiz-Ripa Paula Eguizábal Allelen Campaña-Burguet Emilia Cercenado Ana Isabel López-Calleja Javier Castillo Jose Manuel Azcona-Gutiérrez Luis Torres Jorge Calvo Carmen Martin María Navarro Myriam Zarazaga Carmen Torres the Study Group of Clinical LA-MRSA |
| author_facet | Sara Ceballos Carmen Lozano Carmen Aspiroz Laura Ruiz-Ripa Paula Eguizábal Allelen Campaña-Burguet Emilia Cercenado Ana Isabel López-Calleja Javier Castillo Jose Manuel Azcona-Gutiérrez Luis Torres Jorge Calvo Carmen Martin María Navarro Myriam Zarazaga Carmen Torres the Study Group of Clinical LA-MRSA |
| author_sort | Sara Ceballos |
| collection | DOAJ |
| description | Tetracycline resistance (Tet<sup>R</sup>) has been evidenced as a good phenotypic marker for detection of livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) isolates of the clonal complex CC398. The aim of this study was to characterise a collection of 95 Tet<sup>R</sup>-MRSA isolates, not belonging to the lineage CC398, that were obtained in a previous multicentre study, to detect other MRSA clonal complexes that could be associated with this phenotypic Tet<sup>R</sup> marker. The Tet<sup>R</sup>-MRSA isolates were recovered from 20 Spanish hospitals during 2016 and they were characterised to determine their antimicrobial resistance and virulence phenotypes/genotypes as well as the presence of the immune evasion cluster (IEC). A high proportion of isolates belonging to the CC1 lineage (46%) were observed, as well as to the CC5, CC8 and CC45 lineages (11% each one). Thirty-two different <i>spa</i>-types were identified, being predominantly CC1-t127 (40%) and CC45-t1081 (11%). The IEC system (with the gene <i>scn</i> as marker) was present in 73% of isolates and 16% produced the Panton Valentine leucocidin (PVL). A high proportion of MRSA-CC1 isolates were <i>scn</i>-negative (38.6%) and 52.9% of them were <i>blaZ</i>-negative. A multidrug resistance (MDR) phenotype was identified in 86% of MRSA isolates. The knowledge of other Tet<sup>R</sup>-MRSA genetic lineages, in addition to CC398, is highly relevant, since most of them were MDR and some of them presented important virulence factors. Strains potentially associated with livestock (as the subpopulation CC1-t127-<i>scn</i>-negative) or with humans (as the CC45 lineage or the subpopulation CC1-<i>scn</i>-positive) have been found in this study. The use of tetracycline-resistance for detection, not only of CC398 but also of other LA-MRSA lineages should be tracked in the future. |
| first_indexed | 2024-03-09T13:03:14Z |
| format | Article |
| id | doaj.art-dfce4f53e43f41729dd3cbaeb8bdf1eb |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T13:03:14Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-dfce4f53e43f41729dd3cbaeb8bdf1eb2023-11-30T21:52:14ZengMDPI AGPathogens2076-08172022-03-0111330710.3390/pathogens11030307Beyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant <em>Staphylococcus aureus</em> Genetic Lineages Circulating in Spanish HospitalsSara Ceballos0Carmen Lozano1Carmen Aspiroz2Laura Ruiz-Ripa3Paula Eguizábal4Allelen Campaña-Burguet5Emilia Cercenado6Ana Isabel López-Calleja7Javier Castillo8Jose Manuel Azcona-Gutiérrez9Luis Torres10Jorge Calvo11Carmen Martin12María Navarro13Myriam Zarazaga14Carmen Torres15the Study Group of Clinical LA-MRSAArea Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, SpainArea Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, SpainServicio de Microbiología, Hospital Royo Villanova, 50015 Zaragoza, SpainArea Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, SpainArea Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, SpainArea Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, SpainServicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, CIBERES (CIBER de Enfermedades Respiratorias), 28007 Madrid, SpainHospital Universitario Miguel Servet/IIS Aragón, 50009 Zaragoza, SpainHospital Clínico Universitario Lozano Blesa, 50009 Zaragoza, SpainHospital San Pedro, 26006 Logroño, SpainHospital San Jorge, 22004 Huesca, SpainHospital Universitario Marqués de Valdecilla, CIBERINFEC (CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III), 39008 Santander, SpainComplejo Hospitalario de Navarra, 31008 Pamplona, SpainHospital Universitari de Vic, 08500 Vic, SpainArea Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, SpainArea Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, SpainTetracycline resistance (Tet<sup>R</sup>) has been evidenced as a good phenotypic marker for detection of livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) isolates of the clonal complex CC398. The aim of this study was to characterise a collection of 95 Tet<sup>R</sup>-MRSA isolates, not belonging to the lineage CC398, that were obtained in a previous multicentre study, to detect other MRSA clonal complexes that could be associated with this phenotypic Tet<sup>R</sup> marker. The Tet<sup>R</sup>-MRSA isolates were recovered from 20 Spanish hospitals during 2016 and they were characterised to determine their antimicrobial resistance and virulence phenotypes/genotypes as well as the presence of the immune evasion cluster (IEC). A high proportion of isolates belonging to the CC1 lineage (46%) were observed, as well as to the CC5, CC8 and CC45 lineages (11% each one). Thirty-two different <i>spa</i>-types were identified, being predominantly CC1-t127 (40%) and CC45-t1081 (11%). The IEC system (with the gene <i>scn</i> as marker) was present in 73% of isolates and 16% produced the Panton Valentine leucocidin (PVL). A high proportion of MRSA-CC1 isolates were <i>scn</i>-negative (38.6%) and 52.9% of them were <i>blaZ</i>-negative. A multidrug resistance (MDR) phenotype was identified in 86% of MRSA isolates. The knowledge of other Tet<sup>R</sup>-MRSA genetic lineages, in addition to CC398, is highly relevant, since most of them were MDR and some of them presented important virulence factors. Strains potentially associated with livestock (as the subpopulation CC1-t127-<i>scn</i>-negative) or with humans (as the CC45 lineage or the subpopulation CC1-<i>scn</i>-positive) have been found in this study. The use of tetracycline-resistance for detection, not only of CC398 but also of other LA-MRSA lineages should be tracked in the future.https://www.mdpi.com/2076-0817/11/3/307MRSAtetracycline resistanceCC398CC1t127multicentre study |
| spellingShingle | Sara Ceballos Carmen Lozano Carmen Aspiroz Laura Ruiz-Ripa Paula Eguizábal Allelen Campaña-Burguet Emilia Cercenado Ana Isabel López-Calleja Javier Castillo Jose Manuel Azcona-Gutiérrez Luis Torres Jorge Calvo Carmen Martin María Navarro Myriam Zarazaga Carmen Torres the Study Group of Clinical LA-MRSA Beyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant <em>Staphylococcus aureus</em> Genetic Lineages Circulating in Spanish Hospitals Pathogens MRSA tetracycline resistance CC398 CC1 t127 multicentre study |
| title | Beyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant <em>Staphylococcus aureus</em> Genetic Lineages Circulating in Spanish Hospitals |
| title_full | Beyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant <em>Staphylococcus aureus</em> Genetic Lineages Circulating in Spanish Hospitals |
| title_fullStr | Beyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant <em>Staphylococcus aureus</em> Genetic Lineages Circulating in Spanish Hospitals |
| title_full_unstemmed | Beyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant <em>Staphylococcus aureus</em> Genetic Lineages Circulating in Spanish Hospitals |
| title_short | Beyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant <em>Staphylococcus aureus</em> Genetic Lineages Circulating in Spanish Hospitals |
| title_sort | beyond cc398 characterisation of other tetracycline and methicillin resistant em staphylococcus aureus em genetic lineages circulating in spanish hospitals |
| topic | MRSA tetracycline resistance CC398 CC1 t127 multicentre study |
| url | https://www.mdpi.com/2076-0817/11/3/307 |
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