Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel <i>Xylaria</i> Species Associated with Termite Nests and LC-MS Analysis
<i>Xylaria</i> species associated with termite nests or soil have been considered rare species in nature and the few which have been reported upon have been found to act as a rich source of bioactive metabolites. This study evaluated 10 ethyl acetate extracts of five new <i>Xylaria...
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MDPI AG
2021-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/10/1557 |
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| author | Niwana Wangsawat Lutfun Nahar Satyajit D. Sarker Cherdchai Phosri Andrew R. Evans Anthony J. S. Whalley Kiattawee Choowongkomon Nuttika Suwannasai |
| author_facet | Niwana Wangsawat Lutfun Nahar Satyajit D. Sarker Cherdchai Phosri Andrew R. Evans Anthony J. S. Whalley Kiattawee Choowongkomon Nuttika Suwannasai |
| author_sort | Niwana Wangsawat |
| collection | DOAJ |
| description | <i>Xylaria</i> species associated with termite nests or soil have been considered rare species in nature and the few which have been reported upon have been found to act as a rich source of bioactive metabolites. This study evaluated 10 ethyl acetate extracts of five new <i>Xylaria</i> species associated with termite nests or soil for their antioxidant activity, and cytotoxicity against different cancer and normal cell lines. DPPH and ABTS radical scavenging activities of the extracts demonstrated strong capacity with low IC<sub>50</sub> values. The highest observed activities belonged to <i>X. vinacea</i> SWUF18-2.3 having IC<sub>50</sub> values of 0.194 ± 0.031 mg/mL for DPPH assay and 0.020 ± 0.004 mg/mL for ABTS assay. Total phenolic content ranged from 0.826 ± 0.123 to 3.629 ± 0.381 g GAE/g crude extract which correlated with antioxidant activities. The high total phenolic content could contribute to the high antioxidant activities. Cytotoxicity was recorded against A549, HepG2, HeLa and PNT2 and resulted in broad spectrum to specific activity depending on the cell lines. The highest activities were observed with <i>X. subintraflava</i> SWUF16-11.1 which resulted in 11.15 ± 0.32 to 13.17 ± 2.37% cell viability at a concentration of 100 µg/mL. Moreover, LC-MS fingerprints indicated over 61 peaks from all isolates. There were 18 identified and 43 unidentified compounds compared to mass databases. The identified compounds were from various groups of diterpenoids, diterpenes, cytochalasin, flavones, flavonoids, polyphenols, steroids and derivatives, triterpenoids and tropones. These results indicate that <i>Xylaria</i> spp. has abundant secondary metabolites that could be further explored for their therapeutic properties. |
| first_indexed | 2024-03-10T06:45:47Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T06:45:47Z |
| publishDate | 2021-09-01 |
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| series | Antioxidants |
| spelling | doaj.art-dfd06799244545b9b1900b07d56ed2452023-11-22T17:15:49ZengMDPI AGAntioxidants2076-39212021-09-011010155710.3390/antiox10101557Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel <i>Xylaria</i> Species Associated with Termite Nests and LC-MS AnalysisNiwana Wangsawat0Lutfun Nahar1Satyajit D. Sarker2Cherdchai Phosri3Andrew R. Evans4Anthony J. S. Whalley5Kiattawee Choowongkomon6Nuttika Suwannasai7Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, ThailandLaboratory of Growth Regulators, Institute of Experimental Botany ASCR & Palacký University, Šlechtitelů 27, 78371 Olomouc, Czech RepublicCentre for Natural Products Discovery (CNPD), School of Pharmacy and Bimolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, UKDepartment of Biology, Faculty of Science, Nakhon Phanom University, Nakhon Phanom 48000, ThailandCentre for Natural Products Discovery (CNPD), School of Pharmacy and Bimolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, UKCentre for Natural Products Discovery (CNPD), School of Pharmacy and Bimolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, UKDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandDepartment of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand<i>Xylaria</i> species associated with termite nests or soil have been considered rare species in nature and the few which have been reported upon have been found to act as a rich source of bioactive metabolites. This study evaluated 10 ethyl acetate extracts of five new <i>Xylaria</i> species associated with termite nests or soil for their antioxidant activity, and cytotoxicity against different cancer and normal cell lines. DPPH and ABTS radical scavenging activities of the extracts demonstrated strong capacity with low IC<sub>50</sub> values. The highest observed activities belonged to <i>X. vinacea</i> SWUF18-2.3 having IC<sub>50</sub> values of 0.194 ± 0.031 mg/mL for DPPH assay and 0.020 ± 0.004 mg/mL for ABTS assay. Total phenolic content ranged from 0.826 ± 0.123 to 3.629 ± 0.381 g GAE/g crude extract which correlated with antioxidant activities. The high total phenolic content could contribute to the high antioxidant activities. Cytotoxicity was recorded against A549, HepG2, HeLa and PNT2 and resulted in broad spectrum to specific activity depending on the cell lines. The highest activities were observed with <i>X. subintraflava</i> SWUF16-11.1 which resulted in 11.15 ± 0.32 to 13.17 ± 2.37% cell viability at a concentration of 100 µg/mL. Moreover, LC-MS fingerprints indicated over 61 peaks from all isolates. There were 18 identified and 43 unidentified compounds compared to mass databases. The identified compounds were from various groups of diterpenoids, diterpenes, cytochalasin, flavones, flavonoids, polyphenols, steroids and derivatives, triterpenoids and tropones. These results indicate that <i>Xylaria</i> spp. has abundant secondary metabolites that could be further explored for their therapeutic properties.https://www.mdpi.com/2076-3921/10/10/1557antioxidantcytotoxicityLC-MS fingerprint<i>Xylaria</i>termite nest |
| spellingShingle | Niwana Wangsawat Lutfun Nahar Satyajit D. Sarker Cherdchai Phosri Andrew R. Evans Anthony J. S. Whalley Kiattawee Choowongkomon Nuttika Suwannasai Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel <i>Xylaria</i> Species Associated with Termite Nests and LC-MS Analysis Antioxidants antioxidant cytotoxicity LC-MS fingerprint <i>Xylaria</i> termite nest |
| title | Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel <i>Xylaria</i> Species Associated with Termite Nests and LC-MS Analysis |
| title_full | Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel <i>Xylaria</i> Species Associated with Termite Nests and LC-MS Analysis |
| title_fullStr | Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel <i>Xylaria</i> Species Associated with Termite Nests and LC-MS Analysis |
| title_full_unstemmed | Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel <i>Xylaria</i> Species Associated with Termite Nests and LC-MS Analysis |
| title_short | Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel <i>Xylaria</i> Species Associated with Termite Nests and LC-MS Analysis |
| title_sort | antioxidant activity and cytotoxicity against cancer cell lines of the extracts from novel i xylaria i species associated with termite nests and lc ms analysis |
| topic | antioxidant cytotoxicity LC-MS fingerprint <i>Xylaria</i> termite nest |
| url | https://www.mdpi.com/2076-3921/10/10/1557 |
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