In vivo interactome profiling by enzyme‐catalyzed proximity labeling
Abstract Enzyme-catalyzed proximity labeling (PL) combined with mass spectrometry (MS) has emerged as a revolutionary approach to reveal the protein-protein interaction networks, dissect complex biological processes, and characterize the subcellular proteome in a more physiological setting than befo...
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | Cell & Bioscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13578-021-00542-3 |
| _version_ | 1818969011561431040 |
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| author | Yangfan Xu Xianqun Fan Yang Hu |
| author_facet | Yangfan Xu Xianqun Fan Yang Hu |
| author_sort | Yangfan Xu |
| collection | DOAJ |
| description | Abstract Enzyme-catalyzed proximity labeling (PL) combined with mass spectrometry (MS) has emerged as a revolutionary approach to reveal the protein-protein interaction networks, dissect complex biological processes, and characterize the subcellular proteome in a more physiological setting than before. The enzymatic tags are being upgraded to improve temporal and spatial resolution and obtain faster catalytic dynamics and higher catalytic efficiency. In vivo application of PL integrated with other state of the art techniques has recently been adapted in live animals and plants, allowing questions to be addressed that were previously inaccessible. It is timely to summarize the current state of PL-dependent interactome studies and their potential applications. We will focus on in vivo uses of newer versions of PL and highlight critical considerations for successful in vivo PL experiments that will provide novel insights into the protein interactome in the context of human diseases. |
| first_indexed | 2024-12-20T14:13:48Z |
| format | Article |
| id | doaj.art-dfd25dbda19249f4863a844b2edded5a |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-12-20T14:13:48Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj.art-dfd25dbda19249f4863a844b2edded5a2022-12-21T19:38:05ZengBMCCell & Bioscience2045-37012021-01-011111910.1186/s13578-021-00542-3In vivo interactome profiling by enzyme‐catalyzed proximity labelingYangfan Xu0Xianqun Fan1Yang Hu2Department of Ophthalmology, Stanford University School of MedicineDepartment of Ophthalmology, Ninth People’s Hospital, Shanghai JiaoTong University School of MedicineDepartment of Ophthalmology, Stanford University School of MedicineAbstract Enzyme-catalyzed proximity labeling (PL) combined with mass spectrometry (MS) has emerged as a revolutionary approach to reveal the protein-protein interaction networks, dissect complex biological processes, and characterize the subcellular proteome in a more physiological setting than before. The enzymatic tags are being upgraded to improve temporal and spatial resolution and obtain faster catalytic dynamics and higher catalytic efficiency. In vivo application of PL integrated with other state of the art techniques has recently been adapted in live animals and plants, allowing questions to be addressed that were previously inaccessible. It is timely to summarize the current state of PL-dependent interactome studies and their potential applications. We will focus on in vivo uses of newer versions of PL and highlight critical considerations for successful in vivo PL experiments that will provide novel insights into the protein interactome in the context of human diseases.https://doi.org/10.1186/s13578-021-00542-3Proximity labelingProtein interactomeAPEXBioIDTurboID |
| spellingShingle | Yangfan Xu Xianqun Fan Yang Hu In vivo interactome profiling by enzyme‐catalyzed proximity labeling Cell & Bioscience Proximity labeling Protein interactome APEX BioID TurboID |
| title | In vivo interactome profiling by enzyme‐catalyzed proximity labeling |
| title_full | In vivo interactome profiling by enzyme‐catalyzed proximity labeling |
| title_fullStr | In vivo interactome profiling by enzyme‐catalyzed proximity labeling |
| title_full_unstemmed | In vivo interactome profiling by enzyme‐catalyzed proximity labeling |
| title_short | In vivo interactome profiling by enzyme‐catalyzed proximity labeling |
| title_sort | in vivo interactome profiling by enzyme catalyzed proximity labeling |
| topic | Proximity labeling Protein interactome APEX BioID TurboID |
| url | https://doi.org/10.1186/s13578-021-00542-3 |
| work_keys_str_mv | AT yangfanxu invivointeractomeprofilingbyenzymecatalyzedproximitylabeling AT xianqunfan invivointeractomeprofilingbyenzymecatalyzedproximitylabeling AT yanghu invivointeractomeprofilingbyenzymecatalyzedproximitylabeling |