Dynamics, Diversity, and Virulence of Aeromonas spp. in Homestead Pond Water in Coastal Bangladesh
Aeromonads are aquatic bacteria associated with frequent outbreaks of diarrhea in coastal Bangladesh, but their potential risks from environmental sources have remained largely unexplored. This study, over 2 years, examined homestead pond waters in the region for monthly dynamics and diversity of Ae...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2021.692166/full |
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| author | Abdus Sadique Sucharit Basu Neogi Tanvir Bashar Marzia Sultana Fatema-Tuz Johura Saiful Islam Nur A. Hasan Nur A. Hasan Anwar Huq Rita R. Colwell Rita R. Colwell Rita R. Colwell Rita R. Colwell Munirul Alam |
| author_facet | Abdus Sadique Sucharit Basu Neogi Tanvir Bashar Marzia Sultana Fatema-Tuz Johura Saiful Islam Nur A. Hasan Nur A. Hasan Anwar Huq Rita R. Colwell Rita R. Colwell Rita R. Colwell Rita R. Colwell Munirul Alam |
| author_sort | Abdus Sadique |
| collection | DOAJ |
| description | Aeromonads are aquatic bacteria associated with frequent outbreaks of diarrhea in coastal Bangladesh, but their potential risks from environmental sources have remained largely unexplored. This study, over 2 years, examined homestead pond waters in the region for monthly dynamics and diversity of Aeromonas spp. The bacterial counts showed bi-modal annual growth peak, pre- and post-monsoon, strongly correlating (p < 0.0005) with temperature. Of 200 isolates characterized, Aeromonas veronii bv. sobria (27%) was predominant among co-existent Aeromonas schubertii (20%), Aeromonas hydrophila (17%), Aeromonas caviae (13%), and three more. PCR screening of virulence-related genes identified 15 genotypes (I to XV), however, enterotoxigenicity in animal model was observed for five genotypes, ca. 18% (nine of 50) strains, prevalent in A. veronii bv. sobria, A. hydrophila, and A. caviae. Pathogenic strains were distinguishable by possessing at least three of the major virulence genes: ascV, hlyA, ela, ast, and alt, together with accessory virulence factors. PFGE of XbaI-digested genomic DNA revealed high genetic diversity and distant lineage of potentially toxigenic clones. Therefore, along with increased global warming, Aeromonas spp. having multi-factorial virulence potential in coastal ponds that serve as drinking water sources pose a potential health risk, and underscores the need for routine monitoring. |
| first_indexed | 2024-12-14T20:47:30Z |
| format | Article |
| id | doaj.art-dfd42ae101eb47a1b78c42895d415daf |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-12-14T20:47:30Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj.art-dfd42ae101eb47a1b78c42895d415daf2022-12-21T22:47:59ZengFrontiers Media S.A.Frontiers in Public Health2296-25652021-07-01910.3389/fpubh.2021.692166692166Dynamics, Diversity, and Virulence of Aeromonas spp. in Homestead Pond Water in Coastal BangladeshAbdus Sadique0Sucharit Basu Neogi1Tanvir Bashar2Marzia Sultana3Fatema-Tuz Johura4Saiful Islam5Nur A. Hasan6Nur A. Hasan7Anwar Huq8Rita R. Colwell9Rita R. Colwell10Rita R. Colwell11Rita R. Colwell12Munirul Alam13icddr, b, Formerly International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladeshicddr, b, Formerly International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladeshicddr, b, Formerly International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladeshicddr, b, Formerly International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladeshicddr, b, Formerly International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladeshicddr, b, Formerly International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, BangladeshEzBiome Inc., Gaithersburg, MD, United StatesCenter for Bioinformatics and Computational Biology, University of Maryland, College Park, MD, United StatesMaryland Pathogen Research Institute, University of Maryland, College Park, MD, United StatesEzBiome Inc., Gaithersburg, MD, United StatesCenter for Bioinformatics and Computational Biology, University of Maryland, College Park, MD, United StatesMaryland Pathogen Research Institute, University of Maryland, College Park, MD, United StatesJohns Hopkins Bloomberg School of Public Health, Baltimore, MD, United Statesicddr, b, Formerly International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, BangladeshAeromonads are aquatic bacteria associated with frequent outbreaks of diarrhea in coastal Bangladesh, but their potential risks from environmental sources have remained largely unexplored. This study, over 2 years, examined homestead pond waters in the region for monthly dynamics and diversity of Aeromonas spp. The bacterial counts showed bi-modal annual growth peak, pre- and post-monsoon, strongly correlating (p < 0.0005) with temperature. Of 200 isolates characterized, Aeromonas veronii bv. sobria (27%) was predominant among co-existent Aeromonas schubertii (20%), Aeromonas hydrophila (17%), Aeromonas caviae (13%), and three more. PCR screening of virulence-related genes identified 15 genotypes (I to XV), however, enterotoxigenicity in animal model was observed for five genotypes, ca. 18% (nine of 50) strains, prevalent in A. veronii bv. sobria, A. hydrophila, and A. caviae. Pathogenic strains were distinguishable by possessing at least three of the major virulence genes: ascV, hlyA, ela, ast, and alt, together with accessory virulence factors. PFGE of XbaI-digested genomic DNA revealed high genetic diversity and distant lineage of potentially toxigenic clones. Therefore, along with increased global warming, Aeromonas spp. having multi-factorial virulence potential in coastal ponds that serve as drinking water sources pose a potential health risk, and underscores the need for routine monitoring.https://www.frontiersin.org/articles/10.3389/fpubh.2021.692166/fullAeromonascoastal pondseasonalitydiversitytoxigenic genesvirulence |
| spellingShingle | Abdus Sadique Sucharit Basu Neogi Tanvir Bashar Marzia Sultana Fatema-Tuz Johura Saiful Islam Nur A. Hasan Nur A. Hasan Anwar Huq Rita R. Colwell Rita R. Colwell Rita R. Colwell Rita R. Colwell Munirul Alam Dynamics, Diversity, and Virulence of Aeromonas spp. in Homestead Pond Water in Coastal Bangladesh Frontiers in Public Health Aeromonas coastal pond seasonality diversity toxigenic genes virulence |
| title | Dynamics, Diversity, and Virulence of Aeromonas spp. in Homestead Pond Water in Coastal Bangladesh |
| title_full | Dynamics, Diversity, and Virulence of Aeromonas spp. in Homestead Pond Water in Coastal Bangladesh |
| title_fullStr | Dynamics, Diversity, and Virulence of Aeromonas spp. in Homestead Pond Water in Coastal Bangladesh |
| title_full_unstemmed | Dynamics, Diversity, and Virulence of Aeromonas spp. in Homestead Pond Water in Coastal Bangladesh |
| title_short | Dynamics, Diversity, and Virulence of Aeromonas spp. in Homestead Pond Water in Coastal Bangladesh |
| title_sort | dynamics diversity and virulence of aeromonas spp in homestead pond water in coastal bangladesh |
| topic | Aeromonas coastal pond seasonality diversity toxigenic genes virulence |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2021.692166/full |
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