Histogical structure of the thyroid gland in rabbits with different types of autonomous tonus
The research was aimed at investigating the influence of the aggregated tonus of sympathetic and parasympathetic centers on the rabbit’s thyroid gland morphology. For this purpose, the rabbit males (Oryctolagus cuniculus (Linnaeus, 1758)) of Blanc de Termond breed at the age of four months, passed a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
2020-08-01
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| Series: | Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки |
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| Online Access: | https://nvlvet.com.ua/index.php/journal/article/view/3987 |
| _version_ | 1818554012701556736 |
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| author | M. V. Zakrevska A. M. Tybinka |
| author_facet | M. V. Zakrevska A. M. Tybinka |
| author_sort | M. V. Zakrevska |
| collection | DOAJ |
| description | The research was aimed at investigating the influence of the aggregated tonus of sympathetic and parasympathetic centers on the rabbit’s thyroid gland morphology. For this purpose, the rabbit males (Oryctolagus cuniculus (Linnaeus, 1758)) of Blanc de Termond breed at the age of four months, passed an electrocardiographic and variation pulsometry study. According to the results, the animals were divided into three groups: rabbits with sympathicotonia (18 animals), rabbits with normotonia (5 animals) and rabbits with parasympathicotonia (3 animals). After euthanasia, the fragments of the thyroid gland were taken out, fixed in Bouin’s solution and embedded in paraffin blocks. Histological sections were prepared from the paraffin blocks and later stained with hematoxylin and eosin, as well as Gabu-Diban’s aldehyde fuchsin and Heidenhain’s azan. Morphometric study of follicles and cells of the gland was performed using these histopreparations. Based on the obtained data, the indices of gland activity were calculated, i.e. Brown index and the follicle-colloid index. At the same time, it was established that morphofunctional parameters of the thyroid gland depend on the typological features of the autonomous tonus. This is reflected in the thickness of the capsule, the amount of adipose tissue, the size and density of the follicles’ placement, the height of the thyrocytes and the ratio between the areas of their nuclei and the cytoplasm. Rabbits with parasympathicotonia account for the higher values of half (7 from 14) of the studied parameters, while their combination suggests the functional activity of the thyroid gland of the animals in this group is higher compared to the other groups of rabbits. Also, rabbits with parasympathicotonia have a larger body weight. For normotonic and sympatheticontic rabbits, the functional activity of the thyroid gland as well as the body weight is lower and approximately of the same intensity. The existence of correlation between the separate indicators was investigated, the character of which is also related to the type of the autonomous tonus. |
| first_indexed | 2024-12-12T09:33:30Z |
| format | Article |
| id | doaj.art-dfd489600f8a4069819205644ea1ce85 |
| institution | Directory Open Access Journal |
| issn | 2518-7554 2518-1327 |
| language | English |
| last_indexed | 2024-12-12T09:33:30Z |
| publishDate | 2020-08-01 |
| publisher | Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv |
| record_format | Article |
| series | Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки |
| spelling | doaj.art-dfd489600f8a4069819205644ea1ce852022-12-22T00:28:48ZengStepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies LvivНауковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки2518-75542518-13272020-08-01229811912710.32718/nvlvet98213987Histogical structure of the thyroid gland in rabbits with different types of autonomous tonusM. V. Zakrevska0A. M. Tybinka1Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, UkraineStepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, UkraineThe research was aimed at investigating the influence of the aggregated tonus of sympathetic and parasympathetic centers on the rabbit’s thyroid gland morphology. For this purpose, the rabbit males (Oryctolagus cuniculus (Linnaeus, 1758)) of Blanc de Termond breed at the age of four months, passed an electrocardiographic and variation pulsometry study. According to the results, the animals were divided into three groups: rabbits with sympathicotonia (18 animals), rabbits with normotonia (5 animals) and rabbits with parasympathicotonia (3 animals). After euthanasia, the fragments of the thyroid gland were taken out, fixed in Bouin’s solution and embedded in paraffin blocks. Histological sections were prepared from the paraffin blocks and later stained with hematoxylin and eosin, as well as Gabu-Diban’s aldehyde fuchsin and Heidenhain’s azan. Morphometric study of follicles and cells of the gland was performed using these histopreparations. Based on the obtained data, the indices of gland activity were calculated, i.e. Brown index and the follicle-colloid index. At the same time, it was established that morphofunctional parameters of the thyroid gland depend on the typological features of the autonomous tonus. This is reflected in the thickness of the capsule, the amount of adipose tissue, the size and density of the follicles’ placement, the height of the thyrocytes and the ratio between the areas of their nuclei and the cytoplasm. Rabbits with parasympathicotonia account for the higher values of half (7 from 14) of the studied parameters, while their combination suggests the functional activity of the thyroid gland of the animals in this group is higher compared to the other groups of rabbits. Also, rabbits with parasympathicotonia have a larger body weight. For normotonic and sympatheticontic rabbits, the functional activity of the thyroid gland as well as the body weight is lower and approximately of the same intensity. The existence of correlation between the separate indicators was investigated, the character of which is also related to the type of the autonomous tonus.https://nvlvet.com.ua/index.php/journal/article/view/3987parasympathicotonic rabbits, normotonic rabbits, sympathicotonic rabbits, thyrocytes. |
| spellingShingle | M. V. Zakrevska A. M. Tybinka Histogical structure of the thyroid gland in rabbits with different types of autonomous tonus Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки parasympathicotonic rabbits, normotonic rabbits, sympathicotonic rabbits, thyrocytes. |
| title | Histogical structure of the thyroid gland in rabbits with different types of autonomous tonus |
| title_full | Histogical structure of the thyroid gland in rabbits with different types of autonomous tonus |
| title_fullStr | Histogical structure of the thyroid gland in rabbits with different types of autonomous tonus |
| title_full_unstemmed | Histogical structure of the thyroid gland in rabbits with different types of autonomous tonus |
| title_short | Histogical structure of the thyroid gland in rabbits with different types of autonomous tonus |
| title_sort | histogical structure of the thyroid gland in rabbits with different types of autonomous tonus |
| topic | parasympathicotonic rabbits, normotonic rabbits, sympathicotonic rabbits, thyrocytes. |
| url | https://nvlvet.com.ua/index.php/journal/article/view/3987 |
| work_keys_str_mv | AT mvzakrevska histogicalstructureofthethyroidglandinrabbitswithdifferenttypesofautonomoustonus AT amtybinka histogicalstructureofthethyroidglandinrabbitswithdifferenttypesofautonomoustonus |