Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens
Broiler breeder hens with efficient feed conversion rate under restricted feed intake (R-hens) or allowed unlimited access to feed (Ad-hens) progressed with cardiac functional failure and suffered early sudden death. A supplement of 69 μg 25-hydroxycholecalciferol (25-OH-D<sub>3</sub>)/k...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/21/8379 |
| _version_ | 1797548500900118528 |
|---|---|
| author | Pao-Chia Chou Yu-Hui Chen Thau-Kiong Chung Rosemary L. Walzem Lih-Shiuh Lai Shuen-Ei Chen |
| author_facet | Pao-Chia Chou Yu-Hui Chen Thau-Kiong Chung Rosemary L. Walzem Lih-Shiuh Lai Shuen-Ei Chen |
| author_sort | Pao-Chia Chou |
| collection | DOAJ |
| description | Broiler breeder hens with efficient feed conversion rate under restricted feed intake (R-hens) or allowed unlimited access to feed (Ad-hens) progressed with cardiac functional failure and suffered early sudden death. A supplement of 69 μg 25-hydroxycholecalciferol (25-OH-D<sub>3</sub>)/kg feed improved heart health and rescued livability in both R- and Ad-hens throughout laying stage (26–60 wks). Improvements occurred through cardiac hypertrophic remodeling, reduced arrhythmias, and pathological cues. Here, we further demonstrated consistently decreased circulating and cardiac IL-6 and IL-1β levels in conjunction with reduced cardiac chemoattraction and leukocyte infiltration by 25-OH-D<sub>3</sub> in Ad-hens and in R-hens at later time points (35 and 47 wks) (<i>p</i> < 0.05). Supplemental 25-OH-D<sub>3</sub> also ameliorated cardiac fibrosis, endoplasmic reticulum (ER) stress, and autophagy, mostly in Ad-hens, as both collagen content and expression of COL3A1, as well as CCAAT box binding enhancer homologous protein (CHOP) and activating transcription factor 6 (ATF6), were consistently decreased, and suppression of microtubule-associated protein 1 light Chain 3 beta (LC3B) and Sequestosome 1 (SQSTM1) was rescued at 35 and 47 wks (<i>p</i> < 0.05). Vitamin D receptor-NF-κB signaling was shown to mediate these beneficial effects. The present results demonstrate that ER stress and autophagic processes along the sequence from inflammation to fibrotic changes contribute to pathological cardiac remodeling and functional compromise by Ad-feed intake. 25-OH-D<sub>3</sub> is an effective anti-inflammatory and anti-fibrotic supplement to ameliorate cardiac pathogenesis in broiler breeder hens. |
| first_indexed | 2024-03-10T15:01:32Z |
| format | Article |
| id | doaj.art-dfd809381576474083c449152783f208 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:01:32Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dfd809381576474083c449152783f2082023-11-20T20:13:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012121837910.3390/ijms21218379Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in HensPao-Chia Chou0Yu-Hui Chen1Thau-Kiong Chung2Rosemary L. Walzem3Lih-Shiuh Lai4Shuen-Ei Chen5Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, TaiwanDepartment of Animal Science, National Chung Hsing University, Taichung 402, TaiwanDSM Nutritional Products Asia Pacific, Singapore 117440, SingaporeDepartment of Poultry Science, Texas A&M University, College Station, TX 77843, USADepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, TaiwanDepartment of Animal Science, National Chung Hsing University, Taichung 402, TaiwanBroiler breeder hens with efficient feed conversion rate under restricted feed intake (R-hens) or allowed unlimited access to feed (Ad-hens) progressed with cardiac functional failure and suffered early sudden death. A supplement of 69 μg 25-hydroxycholecalciferol (25-OH-D<sub>3</sub>)/kg feed improved heart health and rescued livability in both R- and Ad-hens throughout laying stage (26–60 wks). Improvements occurred through cardiac hypertrophic remodeling, reduced arrhythmias, and pathological cues. Here, we further demonstrated consistently decreased circulating and cardiac IL-6 and IL-1β levels in conjunction with reduced cardiac chemoattraction and leukocyte infiltration by 25-OH-D<sub>3</sub> in Ad-hens and in R-hens at later time points (35 and 47 wks) (<i>p</i> < 0.05). Supplemental 25-OH-D<sub>3</sub> also ameliorated cardiac fibrosis, endoplasmic reticulum (ER) stress, and autophagy, mostly in Ad-hens, as both collagen content and expression of COL3A1, as well as CCAAT box binding enhancer homologous protein (CHOP) and activating transcription factor 6 (ATF6), were consistently decreased, and suppression of microtubule-associated protein 1 light Chain 3 beta (LC3B) and Sequestosome 1 (SQSTM1) was rescued at 35 and 47 wks (<i>p</i> < 0.05). Vitamin D receptor-NF-κB signaling was shown to mediate these beneficial effects. The present results demonstrate that ER stress and autophagic processes along the sequence from inflammation to fibrotic changes contribute to pathological cardiac remodeling and functional compromise by Ad-feed intake. 25-OH-D<sub>3</sub> is an effective anti-inflammatory and anti-fibrotic supplement to ameliorate cardiac pathogenesis in broiler breeder hens.https://www.mdpi.com/1422-0067/21/21/837925-hydroxycholecalciferolbroiler breeder hensinflammationcardiac fibrosishypertrophy |
| spellingShingle | Pao-Chia Chou Yu-Hui Chen Thau-Kiong Chung Rosemary L. Walzem Lih-Shiuh Lai Shuen-Ei Chen Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens International Journal of Molecular Sciences 25-hydroxycholecalciferol broiler breeder hens inflammation cardiac fibrosis hypertrophy |
| title | Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens |
| title_full | Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens |
| title_fullStr | Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens |
| title_full_unstemmed | Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens |
| title_short | Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens |
| title_sort | supplemental 25 hydroxycholecalciferol alleviates inflammation and cardiac fibrosis in hens |
| topic | 25-hydroxycholecalciferol broiler breeder hens inflammation cardiac fibrosis hypertrophy |
| url | https://www.mdpi.com/1422-0067/21/21/8379 |
| work_keys_str_mv | AT paochiachou supplemental25hydroxycholecalciferolalleviatesinflammationandcardiacfibrosisinhens AT yuhuichen supplemental25hydroxycholecalciferolalleviatesinflammationandcardiacfibrosisinhens AT thaukiongchung supplemental25hydroxycholecalciferolalleviatesinflammationandcardiacfibrosisinhens AT rosemarylwalzem supplemental25hydroxycholecalciferolalleviatesinflammationandcardiacfibrosisinhens AT lihshiuhlai supplemental25hydroxycholecalciferolalleviatesinflammationandcardiacfibrosisinhens AT shueneichen supplemental25hydroxycholecalciferolalleviatesinflammationandcardiacfibrosisinhens |