Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening
BACKGROUND Different strategies for improvement of malaria control and elimination are based on the blockage of malaria parasite transmission to the mosquito vector. These strategies include the drugs that target the plasmodial sexual stages in humans and the early developmental stages inside mosqu...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2021-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762021000100302&tlng=en |
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| author | Camila Fabbri Alexandre Oliveira Trindade Francy’s Sayara Andrade Macejane Ferreira de Souza Claudia María Ríos-Velásquez Marcus Vinicius Guimarães de Lacerda Wuelton Marcelo Monteiro Fabio Trindade Maranhão Costa Rogerio Amino Stefanie Costa Pinto Lopes |
| author_facet | Camila Fabbri Alexandre Oliveira Trindade Francy’s Sayara Andrade Macejane Ferreira de Souza Claudia María Ríos-Velásquez Marcus Vinicius Guimarães de Lacerda Wuelton Marcelo Monteiro Fabio Trindade Maranhão Costa Rogerio Amino Stefanie Costa Pinto Lopes |
| author_sort | Camila Fabbri |
| collection | DOAJ |
| description | BACKGROUND Different strategies for improvement of malaria control and elimination are based on the blockage of malaria parasite transmission to the mosquito vector. These strategies include the drugs that target the plasmodial sexual stages in humans and the early developmental stages inside mosquitoes. OBJECTIVES Here we tested Malaria Box compounds in order to evaluate their activity against male and female gametocytes in Plasmodium berghei, mosquito infection in P. vivax and ookinete formation in both species. METHODS/FINDINGS The membrane feeding assay and the development of ookinetes by a 24 h ex vivo culture and the ookinete yield per 1000 erythrocytes were used to test transmission-blocking potential of the Malaria Box compounds in P. vivax. For P. berghei we used flow cytometry to evaluate male and female gametocyte time course and fluorescence microscopy to check the ookinete development. The two species used in this study showed similar results concerning the compounds’ activity against gametocytes and ookinetes, which were different from those in P. falciparum. In addition, from the eight Malaria Box compounds tested in both species, compounds MMV665830, MMV665878 and MMV665941 were selected as a hit compounds due the high inhibition observed. CONCLUSION Our results showed that P. berghei is suitable as an initial screening system to test compounds against P. vivax. |
| first_indexed | 2024-03-12T09:31:36Z |
| format | Article |
| id | doaj.art-dfd8bc2631b3404f96984a1f101e9ce2 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:31:36Z |
| publishDate | 2021-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-dfd8bc2631b3404f96984a1f101e9ce22023-09-02T13:54:13ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602021-02-0111610.1590/0074-02760200513Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screeningCamila FabbriAlexandre Oliveira TrindadeFrancy’s Sayara AndradeMacejane Ferreira de SouzaClaudia María Ríos-VelásquezMarcus Vinicius Guimarães de LacerdaWuelton Marcelo MonteiroFabio Trindade Maranhão CostaRogerio AminoStefanie Costa Pinto Lopeshttps://orcid.org/0000-0002-6981-2192BACKGROUND Different strategies for improvement of malaria control and elimination are based on the blockage of malaria parasite transmission to the mosquito vector. These strategies include the drugs that target the plasmodial sexual stages in humans and the early developmental stages inside mosquitoes. OBJECTIVES Here we tested Malaria Box compounds in order to evaluate their activity against male and female gametocytes in Plasmodium berghei, mosquito infection in P. vivax and ookinete formation in both species. METHODS/FINDINGS The membrane feeding assay and the development of ookinetes by a 24 h ex vivo culture and the ookinete yield per 1000 erythrocytes were used to test transmission-blocking potential of the Malaria Box compounds in P. vivax. For P. berghei we used flow cytometry to evaluate male and female gametocyte time course and fluorescence microscopy to check the ookinete development. The two species used in this study showed similar results concerning the compounds’ activity against gametocytes and ookinetes, which were different from those in P. falciparum. In addition, from the eight Malaria Box compounds tested in both species, compounds MMV665830, MMV665878 and MMV665941 were selected as a hit compounds due the high inhibition observed. CONCLUSION Our results showed that P. berghei is suitable as an initial screening system to test compounds against P. vivax.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762021000100302&tlng=enMalaria Boxmembrane feeding assay and gametocytes |
| spellingShingle | Camila Fabbri Alexandre Oliveira Trindade Francy’s Sayara Andrade Macejane Ferreira de Souza Claudia María Ríos-Velásquez Marcus Vinicius Guimarães de Lacerda Wuelton Marcelo Monteiro Fabio Trindade Maranhão Costa Rogerio Amino Stefanie Costa Pinto Lopes Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening Memorias do Instituto Oswaldo Cruz Malaria Box membrane feeding assay and gametocytes |
| title | Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening |
| title_full | Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening |
| title_fullStr | Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening |
| title_full_unstemmed | Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening |
| title_short | Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening |
| title_sort | transmission blocking compound candidates against plasmodium vivax using p berghei as an initial screening |
| topic | Malaria Box membrane feeding assay and gametocytes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762021000100302&tlng=en |
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