Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells
The objective of this study was to investigate the protective effects and potential molecular mechanisms of procyanidin B2 (PB2) in MAC-T (mammary alveolar cells-large T antigen) cells during heat stress (HS). The MAC-T cells were divided into three treatment groups: control (37 °C), HS (42 °C), and...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/14/7769 |
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| author | Hongzhuang Wang Weiguang Hao Liang Yang Tingting Li Chongchong Zhao Peishi Yan Shengjuan Wei |
| author_facet | Hongzhuang Wang Weiguang Hao Liang Yang Tingting Li Chongchong Zhao Peishi Yan Shengjuan Wei |
| author_sort | Hongzhuang Wang |
| collection | DOAJ |
| description | The objective of this study was to investigate the protective effects and potential molecular mechanisms of procyanidin B2 (PB2) in MAC-T (mammary alveolar cells-large T antigen) cells during heat stress (HS). The MAC-T cells were divided into three treatment groups: control (37 °C), HS (42 °C), and PB2 + HS (42 °C). Compared with MAC-T cells that were consistently cultured at 37 °C, acute HS treatment remarkably decreased cell viability, reduced activities of catalase (CAT), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC), and elevated intracellular levels of malondialdehyde (MDA) and reactive oxygen species (ROS). Additionally, nuclear factor erythroid 2-related factor 2 (Nrf2) was activated and translocated to the nucleus, in accompaniment with upregulation of Nrf2, heme oxygenase 1 (HO-1), thioredoxin reductase 1 (Txnrd1), and heat shock protein 70 (HSP70). In parallel, both mRNA transcript and actual protein secretion of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), were increased by heat stress. Pretreatment of MAC-T cells with 0~25 μM PB2 alleviated the decline of cell viability by HS in a dose-dependent fashion and protected cells against HS-induced oxidative stress, as evidenced by significantly improved CAT, SOD, and T-AOC activity, as well as with decreased MDA and ROS generation. Furthermore, PB2 further activated the Nrf2 signaling pathway and reversed the inflammatory response induced by HS. Silencing of Nrf2 by si-Nrf2 transfection not only exacerbated HS-induced cell death and provoked oxidative stress and the inflammatory response, but also greatly abolished the cytoprotective effects under HS of PB2. In summary, PB2 protected MAC-T cells against HS-induced cell death, oxidative stress, and inflammatory response, partially by operating at the Nrf2 signal pathway. |
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| language | English |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dfd9726a9c1b4f7b902170a9f50c2f2e2023-12-03T15:09:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012314776910.3390/ijms23147769Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial CellsHongzhuang Wang0Weiguang Hao1Liang Yang2Tingting Li3Chongchong Zhao4Peishi Yan5Shengjuan Wei6College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaThe objective of this study was to investigate the protective effects and potential molecular mechanisms of procyanidin B2 (PB2) in MAC-T (mammary alveolar cells-large T antigen) cells during heat stress (HS). The MAC-T cells were divided into three treatment groups: control (37 °C), HS (42 °C), and PB2 + HS (42 °C). Compared with MAC-T cells that were consistently cultured at 37 °C, acute HS treatment remarkably decreased cell viability, reduced activities of catalase (CAT), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC), and elevated intracellular levels of malondialdehyde (MDA) and reactive oxygen species (ROS). Additionally, nuclear factor erythroid 2-related factor 2 (Nrf2) was activated and translocated to the nucleus, in accompaniment with upregulation of Nrf2, heme oxygenase 1 (HO-1), thioredoxin reductase 1 (Txnrd1), and heat shock protein 70 (HSP70). In parallel, both mRNA transcript and actual protein secretion of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), were increased by heat stress. Pretreatment of MAC-T cells with 0~25 μM PB2 alleviated the decline of cell viability by HS in a dose-dependent fashion and protected cells against HS-induced oxidative stress, as evidenced by significantly improved CAT, SOD, and T-AOC activity, as well as with decreased MDA and ROS generation. Furthermore, PB2 further activated the Nrf2 signaling pathway and reversed the inflammatory response induced by HS. Silencing of Nrf2 by si-Nrf2 transfection not only exacerbated HS-induced cell death and provoked oxidative stress and the inflammatory response, but also greatly abolished the cytoprotective effects under HS of PB2. In summary, PB2 protected MAC-T cells against HS-induced cell death, oxidative stress, and inflammatory response, partially by operating at the Nrf2 signal pathway.https://www.mdpi.com/1422-0067/23/14/7769heat stressmammary epithelial cellsprocyanidin B2Nrf2 |
| spellingShingle | Hongzhuang Wang Weiguang Hao Liang Yang Tingting Li Chongchong Zhao Peishi Yan Shengjuan Wei Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells International Journal of Molecular Sciences heat stress mammary epithelial cells procyanidin B2 Nrf2 |
| title | Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells |
| title_full | Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells |
| title_fullStr | Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells |
| title_full_unstemmed | Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells |
| title_short | Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells |
| title_sort | procyanidin b2 alleviates heat induced oxidative stress through the nrf2 pathway in bovine mammary epithelial cells |
| topic | heat stress mammary epithelial cells procyanidin B2 Nrf2 |
| url | https://www.mdpi.com/1422-0067/23/14/7769 |
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