Control of calcium homeostasis in Schistosoma mansoni
Calcium signalling is fundamental for muscular contractility of Schistosoma mansoni. We have previously described the presence of transport ATPases (Na+,K+-ATPase and (Ca2+-Mg2+)-ATPase) and calcium channels (ryanodine receptors - RyR) involved in control of calcium homeostasis in this worm. Here we...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2001-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000900012 |
| _version_ | 1797718573380009984 |
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| author | F Noël VMN Cunha CLM Silva DL Mendonça-Silva |
| author_facet | F Noël VMN Cunha CLM Silva DL Mendonça-Silva |
| author_sort | F Noël |
| collection | DOAJ |
| description | Calcium signalling is fundamental for muscular contractility of Schistosoma mansoni. We have previously described the presence of transport ATPases (Na+,K+-ATPase and (Ca2+-Mg2+)-ATPase) and calcium channels (ryanodine receptors - RyR) involved in control of calcium homeostasis in this worm. Here we briefly review the main technics (ATPase activity, binding with specific radioligands, fluxes of 45Ca2+ and whole worm contractions) and results obtained in order to compare the distribution patterns of these proteins: thapsigargin-sensitive (Ca2+-Mg2+)-ATPase activity and RyR co-purified in P1 and P4 fractions mainly, which is compatible with a sarcoplasmic reticulum localization, while basal ATPase (along with Na+,K+-ATPase) and thapsigargin-resistant (Ca2+-Mg2+)-ATPase have a distinct distribution, indicative of their plasma membrane localization. Finally we attempt to integrate these contributions with data from other groups in order to propose the first synoptic model for control of calcium homeostasis in S. mansoni. |
| first_indexed | 2024-03-12T08:52:20Z |
| format | Article |
| id | doaj.art-dfd97df3373842e29ff52ba204b0a49f |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:52:20Z |
| publishDate | 2001-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-dfd97df3373842e29ff52ba204b0a49f2023-09-02T16:17:49ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602001-09-0196858810.1590/S0074-02762001000900012Control of calcium homeostasis in Schistosoma mansoniF NoëlVMN CunhaCLM SilvaDL Mendonça-SilvaCalcium signalling is fundamental for muscular contractility of Schistosoma mansoni. We have previously described the presence of transport ATPases (Na+,K+-ATPase and (Ca2+-Mg2+)-ATPase) and calcium channels (ryanodine receptors - RyR) involved in control of calcium homeostasis in this worm. Here we briefly review the main technics (ATPase activity, binding with specific radioligands, fluxes of 45Ca2+ and whole worm contractions) and results obtained in order to compare the distribution patterns of these proteins: thapsigargin-sensitive (Ca2+-Mg2+)-ATPase activity and RyR co-purified in P1 and P4 fractions mainly, which is compatible with a sarcoplasmic reticulum localization, while basal ATPase (along with Na+,K+-ATPase) and thapsigargin-resistant (Ca2+-Mg2+)-ATPase have a distinct distribution, indicative of their plasma membrane localization. Finally we attempt to integrate these contributions with data from other groups in order to propose the first synoptic model for control of calcium homeostasis in S. mansoni.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000900012Schistosoma mansonicalciumATPaseryanodine |
| spellingShingle | F Noël VMN Cunha CLM Silva DL Mendonça-Silva Control of calcium homeostasis in Schistosoma mansoni Memorias do Instituto Oswaldo Cruz Schistosoma mansoni calcium ATPase ryanodine |
| title | Control of calcium homeostasis in Schistosoma mansoni |
| title_full | Control of calcium homeostasis in Schistosoma mansoni |
| title_fullStr | Control of calcium homeostasis in Schistosoma mansoni |
| title_full_unstemmed | Control of calcium homeostasis in Schistosoma mansoni |
| title_short | Control of calcium homeostasis in Schistosoma mansoni |
| title_sort | control of calcium homeostasis in schistosoma mansoni |
| topic | Schistosoma mansoni calcium ATPase ryanodine |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000900012 |
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