Ultrasonic Treatment Enhances Germination and Affects Antioxidant Gene Expression in Soybean (<i>Glycine max</i> L. Merr)
Ultrasound technology has been recently used to enhance and increase seed germination with no negative effect on seedling development. This study investigated the effects of ultrasound exposure for 10, 20, and 30 min on seed germination, seedling growth, and gene expression of three soybean varietie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/10/2446 |
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| author | Ayoob Obaid Alfalahi Bushra Shaker Alobaidy Ahmed Abdulwahid Almarie Omar Mahmood Dhanoon Jamal Ragheb Qasem Ali Fadaam Almehemdi Agnieszka Najda |
| author_facet | Ayoob Obaid Alfalahi Bushra Shaker Alobaidy Ahmed Abdulwahid Almarie Omar Mahmood Dhanoon Jamal Ragheb Qasem Ali Fadaam Almehemdi Agnieszka Najda |
| author_sort | Ayoob Obaid Alfalahi |
| collection | DOAJ |
| description | Ultrasound technology has been recently used to enhance and increase seed germination with no negative effect on seedling development. This study investigated the effects of ultrasound exposure for 10, 20, and 30 min on seed germination, seedling growth, and gene expression of three soybean varieties grown under glasshouse conditions. Ultrasonic treatments showed different effects on most of the studied traits compared with the untreated controls. Ultrasonic exposure increased germination percentage, root and shoot lengths, seedling dry matter, and vigor index of the three soybean varieties. Antioxidant gene expression was examined in the seedling tissues and indicated a significant stimulatory effect of ultrasonication on catalase and superoxide dismutase antioxidant gene expression. Scanning electron microscopy results showed multiple changes in soybean varieties. Seed coat rupturing appeared as pores and cracks on the waved seed coat and possibly increased seed germination. Soybean varieties revealed different abilities to germinate, grow, and develop, as well as different antioxidant gene expression in response to ultrasound treatments. In light of the results obtained, ultrasonication can be widely used to include other crops that face serious challenges in germination. |
| first_indexed | 2024-03-09T20:53:58Z |
| format | Article |
| id | doaj.art-dfdbfe25677449ccb5dc4c0972ed0e11 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T20:53:58Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-dfdbfe25677449ccb5dc4c0972ed0e112023-11-23T22:27:20ZengMDPI AGAgronomy2073-43952022-10-011210244610.3390/agronomy12102446Ultrasonic Treatment Enhances Germination and Affects Antioxidant Gene Expression in Soybean (<i>Glycine max</i> L. Merr)Ayoob Obaid Alfalahi0Bushra Shaker Alobaidy1Ahmed Abdulwahid Almarie2Omar Mahmood Dhanoon3Jamal Ragheb Qasem4Ali Fadaam Almehemdi5Agnieszka Najda6Department of Plant Protection, College of Agriculture, University of Anbar, Anbar 55431, IraqDepartment of Filed Crops, College of Agriculture, University of Anbar, Anbar 55431, IraqDepartment of Filed Crops, College of Agriculture, University of Anbar, Anbar 55431, IraqDepartment of Industrial Crops, Directorate of Agricultural Research, Ministry of Agriculture, Baghdad 10001, IraqPlant Protection Department, Faculty of Agriculture, University of Jordan, Amman 19328, JordanCenter of Desert Studies, University of Anbar, Anbar 55431, IraqDepartment of Vegetable and Herbal Crops, University of Life Sciences, 20-950 Lublin, PolandUltrasound technology has been recently used to enhance and increase seed germination with no negative effect on seedling development. This study investigated the effects of ultrasound exposure for 10, 20, and 30 min on seed germination, seedling growth, and gene expression of three soybean varieties grown under glasshouse conditions. Ultrasonic treatments showed different effects on most of the studied traits compared with the untreated controls. Ultrasonic exposure increased germination percentage, root and shoot lengths, seedling dry matter, and vigor index of the three soybean varieties. Antioxidant gene expression was examined in the seedling tissues and indicated a significant stimulatory effect of ultrasonication on catalase and superoxide dismutase antioxidant gene expression. Scanning electron microscopy results showed multiple changes in soybean varieties. Seed coat rupturing appeared as pores and cracks on the waved seed coat and possibly increased seed germination. Soybean varieties revealed different abilities to germinate, grow, and develop, as well as different antioxidant gene expression in response to ultrasound treatments. In light of the results obtained, ultrasonication can be widely used to include other crops that face serious challenges in germination.https://www.mdpi.com/2073-4395/12/10/2446soybeanultrasonicationantioxidant gene expression |
| spellingShingle | Ayoob Obaid Alfalahi Bushra Shaker Alobaidy Ahmed Abdulwahid Almarie Omar Mahmood Dhanoon Jamal Ragheb Qasem Ali Fadaam Almehemdi Agnieszka Najda Ultrasonic Treatment Enhances Germination and Affects Antioxidant Gene Expression in Soybean (<i>Glycine max</i> L. Merr) Agronomy soybean ultrasonication antioxidant gene expression |
| title | Ultrasonic Treatment Enhances Germination and Affects Antioxidant Gene Expression in Soybean (<i>Glycine max</i> L. Merr) |
| title_full | Ultrasonic Treatment Enhances Germination and Affects Antioxidant Gene Expression in Soybean (<i>Glycine max</i> L. Merr) |
| title_fullStr | Ultrasonic Treatment Enhances Germination and Affects Antioxidant Gene Expression in Soybean (<i>Glycine max</i> L. Merr) |
| title_full_unstemmed | Ultrasonic Treatment Enhances Germination and Affects Antioxidant Gene Expression in Soybean (<i>Glycine max</i> L. Merr) |
| title_short | Ultrasonic Treatment Enhances Germination and Affects Antioxidant Gene Expression in Soybean (<i>Glycine max</i> L. Merr) |
| title_sort | ultrasonic treatment enhances germination and affects antioxidant gene expression in soybean i glycine max i l merr |
| topic | soybean ultrasonication antioxidant gene expression |
| url | https://www.mdpi.com/2073-4395/12/10/2446 |
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