Preadaptation of cell numbers for wound healing assays
In vitro wound healing assays are a suitable application to verify the efficiency of pharmaceuticals or growth factors that will be incorporated in or immobilized to e.g. electrospun biomaterials for wound dressings or other biological devices in advance. Thereby, various factors like culture condit...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2021-10-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2021-2075 |
| _version_ | 1811324215786209280 |
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| author | Markhoff Jana Brietzke Andreas Grabow Niels |
| author_facet | Markhoff Jana Brietzke Andreas Grabow Niels |
| author_sort | Markhoff Jana |
| collection | DOAJ |
| description | In vitro wound healing assays are a suitable application to verify the efficiency of pharmaceuticals or growth factors that will be incorporated in or immobilized to e.g. electrospun biomaterials for wound dressings or other biological devices in advance. Thereby, various factors like culture conditions or cell density influence the specific cell proliferation. Hence, to establish a wound healing assay for various cell types, a stepwise adaptation of cell numbers was done for better estimation and comparison of cell density for the validation of the influence of drugs on the wound healing process. Cell proliferation of different tissue relevant cell types was evaluated by impedance measurements and live cell imaging. Cell numbers could be successfully adapted for assay specific cell densities. In general, a universal comparison of biological or chemical materials and agents in vitro may require the creation of appropriate ISO or OECD standards for a consistent and cell specific adaptation or demand of initial cell density. |
| first_indexed | 2024-04-13T14:10:21Z |
| format | Article |
| id | doaj.art-dfdce018bef44d4aa75b133eee32a09c |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-04-13T14:10:21Z |
| publishDate | 2021-10-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-dfdce018bef44d4aa75b133eee32a09c2022-12-22T02:43:48ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042021-10-017229529810.1515/cdbme-2021-2075Preadaptation of cell numbers for wound healing assaysMarkhoff Jana0Brietzke Andreas1Grabow Niels2Institute for Biomedical Engineering, University Medical Center Rostock,Rostock, GermanyInstitute for Biomedical Engineering, University Medical Center Rostock,Rostock, GermanyInstitute for Biomedical Engineering, University Medical Center Rostock,Rostock, GermanyIn vitro wound healing assays are a suitable application to verify the efficiency of pharmaceuticals or growth factors that will be incorporated in or immobilized to e.g. electrospun biomaterials for wound dressings or other biological devices in advance. Thereby, various factors like culture conditions or cell density influence the specific cell proliferation. Hence, to establish a wound healing assay for various cell types, a stepwise adaptation of cell numbers was done for better estimation and comparison of cell density for the validation of the influence of drugs on the wound healing process. Cell proliferation of different tissue relevant cell types was evaluated by impedance measurements and live cell imaging. Cell numbers could be successfully adapted for assay specific cell densities. In general, a universal comparison of biological or chemical materials and agents in vitro may require the creation of appropriate ISO or OECD standards for a consistent and cell specific adaptation or demand of initial cell density.https://doi.org/10.1515/cdbme-2021-2075wound healingimpedancelive cell imagingstandardisation |
| spellingShingle | Markhoff Jana Brietzke Andreas Grabow Niels Preadaptation of cell numbers for wound healing assays Current Directions in Biomedical Engineering wound healing impedance live cell imaging standardisation |
| title | Preadaptation of cell numbers for wound healing assays |
| title_full | Preadaptation of cell numbers for wound healing assays |
| title_fullStr | Preadaptation of cell numbers for wound healing assays |
| title_full_unstemmed | Preadaptation of cell numbers for wound healing assays |
| title_short | Preadaptation of cell numbers for wound healing assays |
| title_sort | preadaptation of cell numbers for wound healing assays |
| topic | wound healing impedance live cell imaging standardisation |
| url | https://doi.org/10.1515/cdbme-2021-2075 |
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