A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein
Developing a simple and reliable method for the detection of the highly concerning antibiotic, Chloramphenicol (CAP), is of great importance to food safety monitoring. In this study, the authors take the advantages of polyethyleneimine-functionalized reduced graphene oxide and gold nanocubs (PEI-rGO...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-02-01
|
| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S026412752030945X |
| _version_ | 1818839100306751488 |
|---|---|
| author | Ming Lu Chenyang Cao Feng Wang Guocong Liu |
| author_facet | Ming Lu Chenyang Cao Feng Wang Guocong Liu |
| author_sort | Ming Lu |
| collection | DOAJ |
| description | Developing a simple and reliable method for the detection of the highly concerning antibiotic, Chloramphenicol (CAP), is of great importance to food safety monitoring. In this study, the authors take the advantages of polyethyleneimine-functionalized reduced graphene oxide and gold nanocubs (PEI-rGO/AuNCs) composite as electrode modification material to develop an electrochemical aptasensor for the detection of CAP using single-stranded DNA-binding protein. PEI-rGO/AuNCs warrants good electrical conductivity and a high surface-to-volume ratio to achieve the purpose of signal amplification. The CAP-binding aptamers were immobilized on the PEI-rGO/AuNCs modified gold electrode via Au-S affinity. Based on the property that single-stranded DNA-binding protein (SSB) can bind to free single-stranded oligonucleotides without binding to folded oligonucleotides, in the presence of CAP, only a small number of SSB will be immobilized on the aptasensor surface results in a larger peak current, which is typically measured at 0.176 V (vs. Ag/AgCl). The assay has a linear response in the 5 pmol·L−1 to 1 μmol·L−1 concentration range, with a 2.08 pmol·L−1 detection limit. Moreover, the proposed method has been applied to detect CAP in spiked chicken samples with satisfactory results. |
| first_indexed | 2024-12-19T03:48:55Z |
| format | Article |
| id | doaj.art-dfdf714f6b3f445c9041c86e9a590050 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-19T03:48:55Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-dfdf714f6b3f445c9041c86e9a5900502022-12-21T20:37:03ZengElsevierMaterials & Design0264-12752021-02-01199109409A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding proteinMing Lu0Chenyang Cao1Feng Wang2Guocong Liu3School of Chemistry and Materials Engineering, Huizhou University, Huizhou 516007, China; Corresponding authors.School of Material and Chemical Engineering, Henan University of Urban Construction, Pingdingshan 467036, ChinaSchool of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, ChinaSchool of Chemistry and Materials Engineering, Huizhou University, Huizhou 516007, China; Corresponding authors.Developing a simple and reliable method for the detection of the highly concerning antibiotic, Chloramphenicol (CAP), is of great importance to food safety monitoring. In this study, the authors take the advantages of polyethyleneimine-functionalized reduced graphene oxide and gold nanocubs (PEI-rGO/AuNCs) composite as electrode modification material to develop an electrochemical aptasensor for the detection of CAP using single-stranded DNA-binding protein. PEI-rGO/AuNCs warrants good electrical conductivity and a high surface-to-volume ratio to achieve the purpose of signal amplification. The CAP-binding aptamers were immobilized on the PEI-rGO/AuNCs modified gold electrode via Au-S affinity. Based on the property that single-stranded DNA-binding protein (SSB) can bind to free single-stranded oligonucleotides without binding to folded oligonucleotides, in the presence of CAP, only a small number of SSB will be immobilized on the aptasensor surface results in a larger peak current, which is typically measured at 0.176 V (vs. Ag/AgCl). The assay has a linear response in the 5 pmol·L−1 to 1 μmol·L−1 concentration range, with a 2.08 pmol·L−1 detection limit. Moreover, the proposed method has been applied to detect CAP in spiked chicken samples with satisfactory results.http://www.sciencedirect.com/science/article/pii/S026412752030945XAptamerCAPPEI-rGOAuNCsElectrochemical |
| spellingShingle | Ming Lu Chenyang Cao Feng Wang Guocong Liu A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein Materials & Design Aptamer CAP PEI-rGO AuNCs Electrochemical |
| title | A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein |
| title_full | A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein |
| title_fullStr | A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein |
| title_full_unstemmed | A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein |
| title_short | A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein |
| title_sort | polyethyleneimine reduced graphene oxide gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single stranded dna binding protein |
| topic | Aptamer CAP PEI-rGO AuNCs Electrochemical |
| url | http://www.sciencedirect.com/science/article/pii/S026412752030945X |
| work_keys_str_mv | AT minglu apolyethyleneiminereducedgrapheneoxidegoldnanocubesbasedelectrochemicalaptasensorforchloramphenicoldetectionusingsinglestrandeddnabindingprotein AT chenyangcao apolyethyleneiminereducedgrapheneoxidegoldnanocubesbasedelectrochemicalaptasensorforchloramphenicoldetectionusingsinglestrandeddnabindingprotein AT fengwang apolyethyleneiminereducedgrapheneoxidegoldnanocubesbasedelectrochemicalaptasensorforchloramphenicoldetectionusingsinglestrandeddnabindingprotein AT guocongliu apolyethyleneiminereducedgrapheneoxidegoldnanocubesbasedelectrochemicalaptasensorforchloramphenicoldetectionusingsinglestrandeddnabindingprotein AT minglu polyethyleneiminereducedgrapheneoxidegoldnanocubesbasedelectrochemicalaptasensorforchloramphenicoldetectionusingsinglestrandeddnabindingprotein AT chenyangcao polyethyleneiminereducedgrapheneoxidegoldnanocubesbasedelectrochemicalaptasensorforchloramphenicoldetectionusingsinglestrandeddnabindingprotein AT fengwang polyethyleneiminereducedgrapheneoxidegoldnanocubesbasedelectrochemicalaptasensorforchloramphenicoldetectionusingsinglestrandeddnabindingprotein AT guocongliu polyethyleneiminereducedgrapheneoxidegoldnanocubesbasedelectrochemicalaptasensorforchloramphenicoldetectionusingsinglestrandeddnabindingprotein |