α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.
Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeu...
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| Format: | Article |
| Language: | English |
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Trakya University
2023-04-01
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| Series: | Trakya University Journal of Natural Sciences |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/download/article-file/2801180 |
| _version_ | 1827767562824318976 |
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| author | Nevin Ulaş Çolak Ali Kandemir Ufuk Özgen Rezzan Aliyazıcıoğlu Merve Badem Şeyda Kanbolat Sıla Sener |
| author_facet | Nevin Ulaş Çolak Ali Kandemir Ufuk Özgen Rezzan Aliyazıcıoğlu Merve Badem Şeyda Kanbolat Sıla Sener |
| author_sort | Nevin Ulaş Çolak |
| collection | DOAJ |
| description | Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeutic agent for colic, fever, gout and rheumatism. The aim of this study was to analyze the volatile components, to determine phenolic compounds and to evaluate α-amylase, α-glucosidase, and lipase inhibitory activities of the endemic plant species Jurinea brevicaulis Boiss. The widely used solid-phase microextraction technique (SPME) was employed for Gas Chromatography-Mass Spectrometry (GC/MS) analysis of the volatile components. Quantitative analysis of phenolic compounds was performed using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). A total of 19 volatile components were specified and o-cymene (10.60 %), β-bisabolene (9.30 %), and sesquicineole (57.5 %) for different terpenes were described as major components. According to the RP-HPLC analysis, sinapic acid, p-coumaric acid and quercetin were determined for the species. IC50 values of the species were determined as 36.59 ± 2.37 μg/mL and 42.56 ± 2.83 μg/mL for α-amylase and α-glucosidase assays, respectively. IC50 value was found as 50.31 ± 3.75 μg/mL with the lipase inhibition analysis. In conclusion, it has been determined that J. brevicaulis included diverse volatile components, three phenolic compounds with antiobesity effect potential, which highlights J. brevicaulis as the up-and-coming candidate of natural product source to be used against obesity. |
| first_indexed | 2024-03-11T12:00:09Z |
| format | Article |
| id | doaj.art-dfdfe2a8f4e0403585f4f1c5e0835cd0 |
| institution | Directory Open Access Journal |
| issn | 2528-9691 |
| language | English |
| last_indexed | 2024-03-11T12:00:09Z |
| publishDate | 2023-04-01 |
| publisher | Trakya University |
| record_format | Article |
| series | Trakya University Journal of Natural Sciences |
| spelling | doaj.art-dfdfe2a8f4e0403585f4f1c5e0835cd02023-11-08T07:27:56ZengTrakya UniversityTrakya University Journal of Natural Sciences2528-96912023-04-01241414910.23902/trkjnat.121165469α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.Nevin Ulaş Çolak0Ali Kandemir1Ufuk Özgen2Rezzan Aliyazıcıoğlu3Merve Badem4Şeyda Kanbolat5Sıla Sener6KARADENİZ TEKNİK ÜNİVERSİTESİERZİNCAN ÜNİVERSİTESİKARADENİZ TEKNİK ÜNİVERSİTESİKARADENİZ TEKNİK ÜNİVERSİTESİKARADENİZ TEKNİK ÜNİVERSİTESİKARADENİZ TEKNİK ÜNİVERSİTESİSAĞLIK BİLİMLERİ ÜNİVERSİTESİ, GÜLHANE ECZACILIK FAKÜLTESİ, GÜLHANE ECZACILIK PR.Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeutic agent for colic, fever, gout and rheumatism. The aim of this study was to analyze the volatile components, to determine phenolic compounds and to evaluate α-amylase, α-glucosidase, and lipase inhibitory activities of the endemic plant species Jurinea brevicaulis Boiss. The widely used solid-phase microextraction technique (SPME) was employed for Gas Chromatography-Mass Spectrometry (GC/MS) analysis of the volatile components. Quantitative analysis of phenolic compounds was performed using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). A total of 19 volatile components were specified and o-cymene (10.60 %), β-bisabolene (9.30 %), and sesquicineole (57.5 %) for different terpenes were described as major components. According to the RP-HPLC analysis, sinapic acid, p-coumaric acid and quercetin were determined for the species. IC50 values of the species were determined as 36.59 ± 2.37 μg/mL and 42.56 ± 2.83 μg/mL for α-amylase and α-glucosidase assays, respectively. IC50 value was found as 50.31 ± 3.75 μg/mL with the lipase inhibition analysis. In conclusion, it has been determined that J. brevicaulis included diverse volatile components, three phenolic compounds with antiobesity effect potential, which highlights J. brevicaulis as the up-and-coming candidate of natural product source to be used against obesity.https://dergipark.org.tr/en/download/article-file/2801180rp-hplclipasejurineaantiobesityspmegc/ms |
| spellingShingle | Nevin Ulaş Çolak Ali Kandemir Ufuk Özgen Rezzan Aliyazıcıoğlu Merve Badem Şeyda Kanbolat Sıla Sener α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss. Trakya University Journal of Natural Sciences rp-hplc lipase jurinea antiobesity spme gc/ms |
| title | α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss. |
| title_full | α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss. |
| title_fullStr | α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss. |
| title_full_unstemmed | α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss. |
| title_short | α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss. |
| title_sort | α amylase α glucosidase and lipase inhibitory properties and phytochemical analysis of endemic plant jurinea brevicaulis boiss |
| topic | rp-hplc lipase jurinea antiobesity spme gc/ms |
| url | https://dergipark.org.tr/en/download/article-file/2801180 |
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