Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancer
Laryngeal cancer (LC) is an aggressive malignancy resistant to drug treatments. It has been postulated that cancer stem cells (CSCs) persist in a unique population of cancer cells involved in tumor progression and drug-resistance. In the present study, the effects of PLOD2 expression on ordinary and...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S193652332200119X |
| _version_ | 1828810574962622464 |
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| author | Meiyan Song Xing Liu Tao Li Yueqin Zhang Xiaoyan Zhao Wen Sun Zhen Li |
| author_facet | Meiyan Song Xing Liu Tao Li Yueqin Zhang Xiaoyan Zhao Wen Sun Zhen Li |
| author_sort | Meiyan Song |
| collection | DOAJ |
| description | Laryngeal cancer (LC) is an aggressive malignancy resistant to drug treatments. It has been postulated that cancer stem cells (CSCs) persist in a unique population of cancer cells involved in tumor progression and drug-resistance. In the present study, the effects of PLOD2 expression on ordinary and Cisplatin (DDP)-resistance (R) cells were investigated in TU686 and TU138 cells and Xenograft model. Cell viability, invasion and cell apoptosis, CD44 and CD133 expressions, MRP1 and P-gp expressions were measured by CCK-8 assay, Transwell, flow cytometry, immunofluorescence and Western blotting respectively. The results of our study demonstrated that suppressing the expression of PLOD2 could meditate LC stem cell-like features by decrease cell viability and invasion, increase apoptotic rate, decrease CD44 and CD133 expressions via Integrin β1. Meanwhile, the inhibition of PLOD2 expression could decrease P-gp and MRP1expression thus markedly regulate DDP-R LC cells stemness and drug-resistance via Integrin β1. Our findings provided a new rationale for subsequent academic and clinical research on LC drug-resistance. |
| first_indexed | 2024-12-12T09:13:51Z |
| format | Article |
| id | doaj.art-dfe13e91112b40549f89494653623222 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-12-12T09:13:51Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-dfe13e91112b40549f894946536232222022-12-22T00:29:27ZengElsevierTranslational Oncology1936-52332022-08-0122101460Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancerMeiyan Song0Xing Liu1Tao Li2Yueqin Zhang3Xiaoyan Zhao4Wen Sun5Zhen Li6Yantaishan Hospital, Yantai, Shandong, ChinaDepartment of Otolaryngology, Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao, Shandong, ChinaDepartment of Otolaryngology, Zibo Municipal Hospital, Zibo, Shandong, ChinaDepartment of Otolaryngology, Yantaishan Hospital, No.10087 Keji Avenue, Laishan District, Yantai, Shandong 264000, ChinaDepartment of Otolaryngology, Yantaishan Hospital, No.10087 Keji Avenue, Laishan District, Yantai, Shandong 264000, ChinaDepartment of Otolaryngology, Yantaishan Hospital, No.10087 Keji Avenue, Laishan District, Yantai, Shandong 264000, ChinaDepartment of Otolaryngology, Yantaishan Hospital, No.10087 Keji Avenue, Laishan District, Yantai, Shandong 264000, China; Corresponding author.Laryngeal cancer (LC) is an aggressive malignancy resistant to drug treatments. It has been postulated that cancer stem cells (CSCs) persist in a unique population of cancer cells involved in tumor progression and drug-resistance. In the present study, the effects of PLOD2 expression on ordinary and Cisplatin (DDP)-resistance (R) cells were investigated in TU686 and TU138 cells and Xenograft model. Cell viability, invasion and cell apoptosis, CD44 and CD133 expressions, MRP1 and P-gp expressions were measured by CCK-8 assay, Transwell, flow cytometry, immunofluorescence and Western blotting respectively. The results of our study demonstrated that suppressing the expression of PLOD2 could meditate LC stem cell-like features by decrease cell viability and invasion, increase apoptotic rate, decrease CD44 and CD133 expressions via Integrin β1. Meanwhile, the inhibition of PLOD2 expression could decrease P-gp and MRP1expression thus markedly regulate DDP-R LC cells stemness and drug-resistance via Integrin β1. Our findings provided a new rationale for subsequent academic and clinical research on LC drug-resistance.http://www.sciencedirect.com/science/article/pii/S193652332200119XPLOD2Integrin β1Laryngeal cancerDrug-resistanceCancer stem cells |
| spellingShingle | Meiyan Song Xing Liu Tao Li Yueqin Zhang Xiaoyan Zhao Wen Sun Zhen Li Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancer Translational Oncology PLOD2 Integrin β1 Laryngeal cancer Drug-resistance Cancer stem cells |
| title | Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancer |
| title_full | Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancer |
| title_fullStr | Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancer |
| title_full_unstemmed | Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancer |
| title_short | Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancer |
| title_sort | silencing plod2 attenuates cancer stem cell like characteristics and cisplatin resistant through integrin β1 in laryngeal cancer |
| topic | PLOD2 Integrin β1 Laryngeal cancer Drug-resistance Cancer stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S193652332200119X |
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