Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa
Abstract Background Alfalfa (Medicago sativa L.) as an important legume plant can quickly produce adventitious roots (ARs) to form new plants by cutting. But the regulatory mechanism of AR formation in alfalfa remains unclear. Results To better understand the rooting process of alfalfa cuttings, pla...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-04-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04168-0 |
| _version_ | 1797850132913324032 |
|---|---|
| author | Ye Ai Xu Qian Xiaoqian Wang Yinglong Chen Tiejun Zhang Yuehui Chao Yan Zhao |
| author_facet | Ye Ai Xu Qian Xiaoqian Wang Yinglong Chen Tiejun Zhang Yuehui Chao Yan Zhao |
| author_sort | Ye Ai |
| collection | DOAJ |
| description | Abstract Background Alfalfa (Medicago sativa L.) as an important legume plant can quickly produce adventitious roots (ARs) to form new plants by cutting. But the regulatory mechanism of AR formation in alfalfa remains unclear. Results To better understand the rooting process of alfalfa cuttings, plant materials from four stages, including initial separation stage (C stage), induction stage (Y stage), AR primordium formation stage (P stage) and AR maturation stage (S stage) were collected and used for RNA-Seq. Meanwhile, three candidate genes (SAUR, VAN3 and EGLC) were selected to explore their roles in AR formation. The numbers of differentially expressed genes (DEGs) of Y-vs-C (9,724) and P-vs-Y groups (6,836) were larger than that of S-vs-P group (150), indicating highly active in the early AR formation during the complicated development process. Pathways related to cell wall and sugar metabolism, root development, cell cycle, stem cell, and protease were identified, indicating that these genes were involved in AR production. A large number of hormone-related genes associated with the formation of alfalfa ARs have also been identified, in which auxin, ABA and brassinosteroids are thought to play key regulatory roles. Comparing with TF database, it was found that AP2/ERF-ERF, bHLH, WRKY, NAC, MYB, C2H2, bZIP, GRAS played a major regulatory role in the production of ARs of alfalfa. Furthermore, three identified genes showed significant promotion effect on AR formation. Conclusions Stimulation of stem basal cells in alfalfa by cutting induced AR production through the regulation of various hormones, transcription factors and kinases. This study provides new insights of AR formation in alfalfa and enriches gene resources in crop planting and cultivation. |
| first_indexed | 2024-04-09T18:56:29Z |
| format | Article |
| id | doaj.art-dfe17c9e0f444136a2e18b3d9381f53b |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-09T18:56:29Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-dfe17c9e0f444136a2e18b3d9381f53b2023-04-09T11:12:04ZengBMCBMC Plant Biology1471-22292023-04-0123112010.1186/s12870-023-04168-0Uncovering early transcriptional regulation during adventitious root formation in Medicago sativaYe Ai0Xu Qian1Xiaoqian Wang2Yinglong Chen3Tiejun Zhang4Yuehui Chao5Yan Zhao6School of Grassland Science, Beijing Forestry UniversityCollege of Life Sciences, University of Chinese Academy of SciencesBeijing Tide Pharmaceutical Co., LtdThe UWA Institute of Agriculture, and UWA School of Agriculture and Environment, The University of Western AustraliaSchool of Grassland Science, Beijing Forestry UniversitySchool of Grassland Science, Beijing Forestry UniversityCollege of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Key Laboratory of Grassland Resources (IMAU), Ministry of EducationAbstract Background Alfalfa (Medicago sativa L.) as an important legume plant can quickly produce adventitious roots (ARs) to form new plants by cutting. But the regulatory mechanism of AR formation in alfalfa remains unclear. Results To better understand the rooting process of alfalfa cuttings, plant materials from four stages, including initial separation stage (C stage), induction stage (Y stage), AR primordium formation stage (P stage) and AR maturation stage (S stage) were collected and used for RNA-Seq. Meanwhile, three candidate genes (SAUR, VAN3 and EGLC) were selected to explore their roles in AR formation. The numbers of differentially expressed genes (DEGs) of Y-vs-C (9,724) and P-vs-Y groups (6,836) were larger than that of S-vs-P group (150), indicating highly active in the early AR formation during the complicated development process. Pathways related to cell wall and sugar metabolism, root development, cell cycle, stem cell, and protease were identified, indicating that these genes were involved in AR production. A large number of hormone-related genes associated with the formation of alfalfa ARs have also been identified, in which auxin, ABA and brassinosteroids are thought to play key regulatory roles. Comparing with TF database, it was found that AP2/ERF-ERF, bHLH, WRKY, NAC, MYB, C2H2, bZIP, GRAS played a major regulatory role in the production of ARs of alfalfa. Furthermore, three identified genes showed significant promotion effect on AR formation. Conclusions Stimulation of stem basal cells in alfalfa by cutting induced AR production through the regulation of various hormones, transcription factors and kinases. This study provides new insights of AR formation in alfalfa and enriches gene resources in crop planting and cultivation.https://doi.org/10.1186/s12870-023-04168-0Medicago sativa L.CuttageAdventitious RootRNA-SeqDifferentially expressed genes |
| spellingShingle | Ye Ai Xu Qian Xiaoqian Wang Yinglong Chen Tiejun Zhang Yuehui Chao Yan Zhao Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa BMC Plant Biology Medicago sativa L. Cuttage Adventitious Root RNA-Seq Differentially expressed genes |
| title | Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa |
| title_full | Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa |
| title_fullStr | Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa |
| title_full_unstemmed | Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa |
| title_short | Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa |
| title_sort | uncovering early transcriptional regulation during adventitious root formation in medicago sativa |
| topic | Medicago sativa L. Cuttage Adventitious Root RNA-Seq Differentially expressed genes |
| url | https://doi.org/10.1186/s12870-023-04168-0 |
| work_keys_str_mv | AT yeai uncoveringearlytranscriptionalregulationduringadventitiousrootformationinmedicagosativa AT xuqian uncoveringearlytranscriptionalregulationduringadventitiousrootformationinmedicagosativa AT xiaoqianwang uncoveringearlytranscriptionalregulationduringadventitiousrootformationinmedicagosativa AT yinglongchen uncoveringearlytranscriptionalregulationduringadventitiousrootformationinmedicagosativa AT tiejunzhang uncoveringearlytranscriptionalregulationduringadventitiousrootformationinmedicagosativa AT yuehuichao uncoveringearlytranscriptionalregulationduringadventitiousrootformationinmedicagosativa AT yanzhao uncoveringearlytranscriptionalregulationduringadventitiousrootformationinmedicagosativa |