Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma
Abstract Background Renal clear cell carcinoma (ccRCC) is one of the most common malignant tumors, whose incidence is increasing year by year. IRF6 plays an important role in the occurrence of tumors, although there is yet no report on its expression in ccRCC. Methods The expression of IRF6 and KIF2...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-05-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-021-01879-y |
| _version_ | 1818864263651917824 |
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| author | Xinwei Ma Xiaoqi Wang Qian Dong Hongquan Pang Jianming Xu Junkang Shen Jianbing Zhu |
| author_facet | Xinwei Ma Xiaoqi Wang Qian Dong Hongquan Pang Jianming Xu Junkang Shen Jianbing Zhu |
| author_sort | Xinwei Ma |
| collection | DOAJ |
| description | Abstract Background Renal clear cell carcinoma (ccRCC) is one of the most common malignant tumors, whose incidence is increasing year by year. IRF6 plays an important role in the occurrence of tumors, although there is yet no report on its expression in ccRCC. Methods The expression of IRF6 and KIF20A in ccRCC was predicted by GEPIA and HAP databases. In addition, GEPIA database predicted the relationship between IRF6 and KIF20A expressions and the pathological staging, overall survival, and disease-free survival of ccRCC. The possible binding sites of IRF6 and KIF20A promoters were predicted by JASPAR database and verified by luciferase and ChIP assays. The specific effects of IRF6 on ccRCC cell proliferation, invasion and apoptosis were subsequently examined at both cellular level and animal level. Results The database predicted down-regulated IRF6 expression in renal carcinoma tissues and its correlation with poor prognosis. IRF6 overexpression inhibited cRCC cell proliferation, invasion and migration. In addition, up-regulated KIF20A expression in renal carcinoma tissues and its association with prognosis were also predicted. Interference with KIF20A inhibited the proliferation, invasion, and migration of ccRCC cells. Finally, we confirmed that KIF20A is a functional target of IRF6 and can partially reverse the effects of IRF6 on the proliferation, invasion and migration of ccRCC cells. Conclusion: Inhibition of KIF20A by transcription factor IRF6 affects cell proliferation, invasion and migration in renal clear cell carcinoma. |
| first_indexed | 2024-12-19T10:28:53Z |
| format | Article |
| id | doaj.art-dfe23a2c5b0647788fbb4b181c381977 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-19T10:28:53Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-dfe23a2c5b0647788fbb4b181c3819772022-12-21T20:25:49ZengBMCCancer Cell International1475-28672021-05-0121111410.1186/s12935-021-01879-yInhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinomaXinwei Ma0Xiaoqi Wang1Qian Dong2Hongquan Pang3Jianming Xu4Junkang Shen5Jianbing Zhu6Department of Radiology, The Second Affiliated Hospital of Soochow UniversityDepartment of Medical Oncology, The First Affiliated Hospital of Anhui University of Science and TechnologyDepartment of Radiology, Suzhou Science and Technology Town Hospital, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical UniversityDepartment of Radiology, Suzhou Science and Technology Town Hospital, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical UniversityDepartment of Radiology, Suzhou Science and Technology Town Hospital, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical UniversityDepartment of Radiology, The Second Affiliated Hospital of Soochow UniversityDepartment of Radiology, Suzhou Science and Technology Town Hospital, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical UniversityAbstract Background Renal clear cell carcinoma (ccRCC) is one of the most common malignant tumors, whose incidence is increasing year by year. IRF6 plays an important role in the occurrence of tumors, although there is yet no report on its expression in ccRCC. Methods The expression of IRF6 and KIF20A in ccRCC was predicted by GEPIA and HAP databases. In addition, GEPIA database predicted the relationship between IRF6 and KIF20A expressions and the pathological staging, overall survival, and disease-free survival of ccRCC. The possible binding sites of IRF6 and KIF20A promoters were predicted by JASPAR database and verified by luciferase and ChIP assays. The specific effects of IRF6 on ccRCC cell proliferation, invasion and apoptosis were subsequently examined at both cellular level and animal level. Results The database predicted down-regulated IRF6 expression in renal carcinoma tissues and its correlation with poor prognosis. IRF6 overexpression inhibited cRCC cell proliferation, invasion and migration. In addition, up-regulated KIF20A expression in renal carcinoma tissues and its association with prognosis were also predicted. Interference with KIF20A inhibited the proliferation, invasion, and migration of ccRCC cells. Finally, we confirmed that KIF20A is a functional target of IRF6 and can partially reverse the effects of IRF6 on the proliferation, invasion and migration of ccRCC cells. Conclusion: Inhibition of KIF20A by transcription factor IRF6 affects cell proliferation, invasion and migration in renal clear cell carcinoma.https://doi.org/10.1186/s12935-021-01879-yRenal clear cell carcinomaIRF6KIF20APrognosisEarly diagnostic target |
| spellingShingle | Xinwei Ma Xiaoqi Wang Qian Dong Hongquan Pang Jianming Xu Junkang Shen Jianbing Zhu Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma Cancer Cell International Renal clear cell carcinoma IRF6 KIF20A Prognosis Early diagnostic target |
| title | Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma |
| title_full | Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma |
| title_fullStr | Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma |
| title_full_unstemmed | Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma |
| title_short | Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma |
| title_sort | inhibition of kif20a by transcription factor irf6 affects the progression of renal clear cell carcinoma |
| topic | Renal clear cell carcinoma IRF6 KIF20A Prognosis Early diagnostic target |
| url | https://doi.org/10.1186/s12935-021-01879-y |
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