UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of <i>Polygonatum sibiricum</i>
The rhizomes of <i>Polygonatum sibiricum</i> are commonly consumed as food and also used as medicine. However, the metabolic profile of <i>P. sibiricum</i> has not been fully revealed yet. Recently, we developed a novel evergreen species of P. sibiricum. The objectives of thi...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/15/4685 |
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| author | Gelian Luo Junhan Lin Weiqing Cheng Zhibin Liu Tingting Yu Bin Yang |
| author_facet | Gelian Luo Junhan Lin Weiqing Cheng Zhibin Liu Tingting Yu Bin Yang |
| author_sort | Gelian Luo |
| collection | DOAJ |
| description | The rhizomes of <i>Polygonatum sibiricum</i> are commonly consumed as food and also used as medicine. However, the metabolic profile of <i>P. sibiricum</i> has not been fully revealed yet. Recently, we developed a novel evergreen species of P. sibiricum. The objectives of this study were to compare the metabolic profiles of two types of <i>P. sibiricum</i>, i.e., the newly developed evergreen type (Gtype) and a wide-type (Wtype), by using UHPLC-Q-Orbitrap-MS-based untargeted metabolomics approach. A total of 263 and 258 compounds in the positive and negative modes of the mass spectra were tentatively identified. Distinctively different metabolomic profiles of these two types of <i>P. sibiricum</i> were also revealed by principal component analysis (PCA) and principal coordinates analysis (PCoA). Furthermore, by using partial least squares discriminant analysis (PLS-DA) modeling, it was found that, as compared with Wtype, Gtype samples had significantly higher content of oxyberberine, proliferin, alpinetin, and grandisin. On the other hand, 15 compounds, including herniarin, kaempferol 7-neohesperidoside, benzyl beta-primeveroside, vanillic acid, biochanin A, neoschaftoside, benzyl gentiobioside, cornuside, hydroxytyrosol-glucuronide, apigenin-pentosyl-glucoside, obacunone, 13-alpha-(21)-epoxyeurycomanone, vulgarin, digitonin, and 3-formylindole, were discovered to have higher abundance in Wtype samples. These distinguishing metabolites suggest the different beneficial health potentials and flavor attributes of the two types of <i>P. sibiricum</i> rhizomes. |
| first_indexed | 2024-03-09T10:06:55Z |
| format | Article |
| id | doaj.art-dfe2b79c44894ba3a6e59d9080948bd2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:06:55Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dfe2b79c44894ba3a6e59d9080948bd22023-12-01T23:02:42ZengMDPI AGMolecules1420-30492022-07-012715468510.3390/molecules27154685UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of <i>Polygonatum sibiricum</i>Gelian Luo0Junhan Lin1Weiqing Cheng2Zhibin Liu3Tingting Yu4Bin Yang5Fujian Vocational College of Bioengineering, Fuzhou 350007, ChinaFujian Vocational College of Bioengineering, Fuzhou 350007, ChinaFujian Vocational College of Bioengineering, Fuzhou 350007, ChinaInstitute of Food Science & Technology, Fuzhou University, Fuzhou 350108, ChinaFujian Vocational College of Bioengineering, Fuzhou 350007, ChinaNanwuyi Professional Cooperative of Chinese Herbal Medicine Planting, Shaowu 354000, ChinaThe rhizomes of <i>Polygonatum sibiricum</i> are commonly consumed as food and also used as medicine. However, the metabolic profile of <i>P. sibiricum</i> has not been fully revealed yet. Recently, we developed a novel evergreen species of P. sibiricum. The objectives of this study were to compare the metabolic profiles of two types of <i>P. sibiricum</i>, i.e., the newly developed evergreen type (Gtype) and a wide-type (Wtype), by using UHPLC-Q-Orbitrap-MS-based untargeted metabolomics approach. A total of 263 and 258 compounds in the positive and negative modes of the mass spectra were tentatively identified. Distinctively different metabolomic profiles of these two types of <i>P. sibiricum</i> were also revealed by principal component analysis (PCA) and principal coordinates analysis (PCoA). Furthermore, by using partial least squares discriminant analysis (PLS-DA) modeling, it was found that, as compared with Wtype, Gtype samples had significantly higher content of oxyberberine, proliferin, alpinetin, and grandisin. On the other hand, 15 compounds, including herniarin, kaempferol 7-neohesperidoside, benzyl beta-primeveroside, vanillic acid, biochanin A, neoschaftoside, benzyl gentiobioside, cornuside, hydroxytyrosol-glucuronide, apigenin-pentosyl-glucoside, obacunone, 13-alpha-(21)-epoxyeurycomanone, vulgarin, digitonin, and 3-formylindole, were discovered to have higher abundance in Wtype samples. These distinguishing metabolites suggest the different beneficial health potentials and flavor attributes of the two types of <i>P. sibiricum</i> rhizomes.https://www.mdpi.com/1420-3049/27/15/4685rhizomes of <i>Polygonatum sibiricum</i>evergreenmetabolomicschemical markersUHPLC-Q-Orbitrap-MS |
| spellingShingle | Gelian Luo Junhan Lin Weiqing Cheng Zhibin Liu Tingting Yu Bin Yang UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of <i>Polygonatum sibiricum</i> Molecules rhizomes of <i>Polygonatum sibiricum</i> evergreen metabolomics chemical markers UHPLC-Q-Orbitrap-MS |
| title | UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of <i>Polygonatum sibiricum</i> |
| title_full | UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of <i>Polygonatum sibiricum</i> |
| title_fullStr | UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of <i>Polygonatum sibiricum</i> |
| title_full_unstemmed | UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of <i>Polygonatum sibiricum</i> |
| title_short | UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of <i>Polygonatum sibiricum</i> |
| title_sort | uhplc q orbitrap ms based metabolomics reveals chemical variations of two types of rhizomes of i polygonatum sibiricum i |
| topic | rhizomes of <i>Polygonatum sibiricum</i> evergreen metabolomics chemical markers UHPLC-Q-Orbitrap-MS |
| url | https://www.mdpi.com/1420-3049/27/15/4685 |
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